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106-02-5

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Data Sheet - G0791
90% viability. 1. Pellet cells by centrifugation for 5 minutes at 200 x g. Re-suspend the cells at a concentration of 3 x 106 to 5 x 106 cells/ml in 50% fresh Gene Therapy Medium- 2 and 50% conditioned
TC RNA Oligonucleotides Synthesis and Application
Sequence: UAAGCACGAAGCUCAGAGUCCCCC. Yield (crude): 560 nmol. 00:00 00:10 00:15 02:15 02:30 02:45 03:30 06:00 02:15 Assemble manifold and dry columns… ...add EDA … …and react for 2 h. Wash
Data Sheet - L4899
is equivalent to approximately 1.3 x 106 light units. The product is supplied as a solution of approximately 10 mg of protein per ml of 25 mM Tris acetate, 5 mM MgSO4, 1 mM EDTA, and 50% (v/v)...
Monoclonal Anti-Human CD61 Clone BL-E6 Purified Mouse Immunoglobulin
handling practices. Product Profile When assayed by flow cytometric analysis, using 5 µl of the antibody to stain 1 x 106 cells, a fluorescence intensity is observed similar to that obtained with saturating
Data Sheet - C6555
146, 1026 (1991). 9. Caldwell, C. W., et al., Am. J. Clin. Pathol., 95, 180 (1991). JWM 02/02 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants
Data Sheet - C7423
handling practices. Performance When assayed by flow cytometric analysis, 5 µl of the monoclonal antibody will stain 1 x 106 cells with a fluorescence intensity and percent positive that is similar
Monoclonal Anti-CD4 antibody produced in mouse (C1805)
determined by dye exclusion (e.g., trypan blue). For each sample, add 100 µl or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) containing cells to be stained. Vortex tube gently
Data Sheet - L1792
Tris-HCl pH 8.0, 1 mM dithiothreitol, 1 mM EDTA, and 10% (v/v) glycerol. Specific Activity: ≥5 x 106 light units/mg protein Unit Definition: One light unit produces a biometer peak height equivalent
EX-CELL CHO Medium (C1707)
greater than 90% viability. 1. Pellet cells by centrifugation for 5 minutes at 200 x g. Re-suspend at a concentration of 5 x 106 cells/ml in a 50:50 mixture of fresh CHO SF Medium and conditioned
Data Sheet - P4124
Arthritis Res., 3, 102-106 (2001). 3. Brouwer, R., et al., J. Biol. Chem., 276, 6177-6184 (2001). 4. Brouwer, R., et al., Arthritis Res., 4, 134-138 (2002). ...
14385C - Technical Bulletin
production? We recommend seeding at 1.2  106 viable cells/mL 18 to 24 hours before transient transfection. Targeted cell density on the day of transfection is 2.4–3.5  106 viable cells/mL and viability 95%
"CDK1/CyclinA2, active (C0244)"
activity- dependent CaMKIV signaling in the consolidation of long-term memory. Cell, 106, 771-783 (2001). BKR,MAM 02/09-1 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc
Data Sheet - F2902
determined by dye exclusion (Trypan Blue, Product No. T 0776). For each sample, add 100 µl or 1 x 106 cells per tube. (Note: If cell preparation contains red blood cells, they can be lysed by incubating
Monoclonal Anti-HA-Biotin antibody produced in mouse (B9183)
., Cell Rep., 33(5), 108342 (2020). ExtrAvidin is a registered trademark of Sigma-Aldrich Co.. TWEEN is a registered trademark of Croda International PLC. NV,AC,GCY 03/21-02 2021
Data Sheet - E2528
799-804 (1992). 5. Jaenicke, R., et al., Folding proteins, in Protein Structure, a Practical Approach, pages 191-223. (Creighton, IRL Press Oxford). RJM/MAM 1/02 Sigma brand
Data Sheet - F3899
Immunol., 88, 243 (1992). 5. Campos, L., et al., Leucocyte Typing III, 470 (1987). 6. Campos, L., et al., Leucocyte Typing III, 473 (1987). KMR/MAM 01/02 ...
Data Sheet - F1527
. Dalchau, R., et al., Leucocyte Typing III, 814 (1987). 5. Dalchau, R., et al., Eur. J. Immunol. 16, 993 (1986). MAM/KMR 03/02 Sigma brand products are...
Monoclonal Anti-CD36 antibody produced in mouse (C4679)
fixation and paraffin embedding. When assayed by flow cytometric analysis, using 5 µl of the antibody to stain 1 x 106 cells, a fluorescence intensity is observed similar to that obtained with saturating
Data Sheet - C7673
as determined by dye exclusion (trypan blue). For each sample, add 100 µl or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) containing cells to be stained. Vortex...
Data Sheet - SAB4200569
Laufman, O., et al., EMBO J., 28, 2006-2017 (2009). 5. Richardson, B.C., et al., Proc. Natl. Acad. Sci. USA, 106, 13329-13334 (2009). ST,RC,PHC 02/13-1
Data Sheet - SAB4200568
Laufman, O., et al., EMBO J., 28, 2006-2017 (2009). 5. Richardson, B.C., et al., Proc. Natl. Acad. Sci. USA, 106, 13329-13334 (2009). ST,RC,PHC 02/13-1
Data Sheet - F6523
staining and fixation should be performed prior to permeabilization. 1. Dispense 1 X 106 positive/negtive control cells and test sample into 7 x 15 mm tubes. 2. Wash cells twice...
Data Sheet - C6680
83 (1991). 9. Abo, T., and Balch, C.M., J. Immunol., 127, 1024 (1981). JWM 5/02 Sigma brand products are...
Data Sheet - H9773
400 µg/ml for higher eukaryotes. For protocols that describe usage of Hygromycin B in units, 1 X 106 units is approximately equal to 900 mg H7772, but each lot should be titered for each application.
Data Sheet - F4149
, Eur. J. Immunol. 16, 993 (1986). 5. Pinkus, G. In: Advances in Immunohistochemistry, DeLellis, R.A. (ed.), p.261, Raven Press (1988). JWM/KMR 06/02 Sigma brand...
Data Sheet - C9062
as deter- mined by dye exclusion (trypan blue). For each sample, add 100 µl or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) con- taining cells to be stained. Vortex tube gently
Data Sheet - H5654
Markl, J. et al., Naturwissenschaften, 78, 512 (1991). 5. Herskovits, T.T., Comp. Biochem. Physiol. B, 91(4), 597-611 (1988). KAA,DS,MAM 02/12-1 2012 Sigma-Aldrich Co. LLC. All rights reserved
Data Sheet - R0137
used for labeling of 1 x 106 human peripheral blood lymphocytes in flow cytometric applications. Notes 1. It is recommended that cells be washed 2X in PBS containing 5% BSA and resuspended to their
Product Information Sheet - F8791
NIH-3T3 lines transformed by the H-ras (S2-721) and trk (106-632) oncogenes, the sensitivity of these cells to 5’dFUrd is notably enhanced.8 5’dFUrd has been shown to enhance uptake of daunorubicin in
Data Sheet - P7650
glutaraldehyde in a molar ratio of approximately 3 protein A molecules to 1 β-galactosidase. Used in a 105 to 106 fold dilution, this conjugate provides extreme sensitivity in the determination of human IgG by using
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