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MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
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std
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Data sheet
LabRoll™ 1 Roll 5M × 115 mm
IST-106-078TS Trial LabSheet™ 5 Sheets 125 mm × 78 mm
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+44 (0)1252 235758
Confidential information © Scientific iST Ltd.
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Growth Characteristics of the expresSFplus Serum-Free Insect Cell Line in EX-CELL420 Serum-Free Media
at higher
densities. Cell densities in EX-CELLTM 420 were typically
around 6 x 106 cells/mL (range 3.7 x 106 to
9.9 x 106 cells/mL) and displayed an average doubling time
of 26 hours. Culture viabilities
EX-CELL 302 Serum-Free Medium for CHO Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
Product Information Sheet - 14591C
served as controls). The
plates were incubated at 37 C, 5% CO2 for 10 days, then
observed on an inverted microscope for CPE.
Viable Cells/mL
(x 106)
Percent Viable Doubling Time
(Hrs)
Average
Standard
EX-CELL 302 Serum-Free Medium for CHO Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL NS0- An Animal-Component Free
Density
(Cells/mL)
Average
% Viability
Average Doubling
Time (Hours)
2.2 x 106
1.2 x 106
2.9 x 106
1.9 x 106
95.5
84.5
92.0
92.6
16.1
20.0
18.6
22.2
www.safcbiosciences.com
Product Information Sheet - 14591C
shaker
flasks?
The average cell density achieved during a 3-day passage is
approximately 2.9 x 106 ± 1 x 106 cells/mL in shaker flasks.
What is the average doubling time of HeLa cells
in EX-CELLTM HeLa
Suspension MDCK Cell Culture in EX-CELL MDCK Serum-Free Medium
approximately 1-3 x 106 cells/mL, with culture viabilities in
excess of 93%, when subcultured every 3 - 4 days (Figure 2).
The growth curve (Figure 3) indicates that maximal cell
densities nearing 5 x 106 cell/mL
B6-WHITE™ MURINE ES CELL LINE
____________________
CATALOG NUMBER:
SCR011 QUANTITY: 5 x 106 cells, passage 10
LOT NUMBER:
CONCENTRATION: 2.5 x 106 cells / vial
BACKGROUND:
The generation
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant EP2 Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
ChemiScreen™ CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT TSH RECEPTOR, GLYCOPROTEIN HORMONE RECEPTOR FAMILY
prepared as in Step 4). Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-10 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense
ChemiScreen™ Calcium-Optimized Stable Cell Line Human Recombinant GPR109A Receptor
prepared as in Step 4). Centrifuge cells at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in 1321N1 Freezing Media (cell densities of
2-10 x 106 are also acceptable if necessary). Dispense
ChemiScreen™ FLASH AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
prepared as in Step 4). Centrifuge cells at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x
106 are also acceptable if necessary). Dispense 1
ChemiScreenTM GLOW AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
prepared as in Step 4). Centrifuge cells at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x
106 are also acceptable if necessary). Dispense 1
ChemiScreen™ Calcium-Optimized Stable Cell Line Human Recombinant sst<sub>2 </sub>Receptor
prepared as in Step 4). Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-1 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense
EX-CELL 610-HSF Serum-Free Medium for Hybridoma Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
ChemiScreen™ Human Recombinant XCR1 Chemokine Receptor Calcium-Optimized Stable Cell Line
prepared as in Step 4). Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-4 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense
EX-CELL 405 Serum-Free Medium for Insect Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL 420 Serum-Free Medium for Insect Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant EP3 Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
EX-CELL CD CHO- A Chemically DefinedAnimal-Component Free Serum-Free Mediumfor CHO Cells
EX-CELLTM CD CHO at a density of
5 x 105 cells/mL. Cells were subcultured every 3 - 4 days and
were considered fully adapted when cell densities achieved
> 2.0 x 106 cells/mL and viabilities of > 95%.
ChemiScreen™ Glow Aequorin Calcium-Optimized Stable Cell Line Human Recombinant TP Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
ChemiScreen™ Glow Aequorin Calcium-Optimized Stable Cell Line Human Recombinant H1 Histamine Receptor
prepared as in Step 4). Centrifuge cells at 200 x g for 5 min.
Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x 106 are also
acceptable if necessary). Dispense 1
EX-CELL 420 Frequently Asked Questions
dimethyl
sulfoxide (DMSO). Centrifuge the cells at 200 g for 5 minutes.
Remove the supernatant. Resuspend the cells in the freezing
medium at 5 x 106 to 1 x 107 cells/mL. Rapidly transfer
1 - 2 mL of this
EX-CELL 620-HSF Serum-Free Medium for Hybridoma Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL Sp2/0- An Animal-ComponentFree Protein-Free Chemically Defined Medium for Monoclonal Antibody Production
recovered and displayed normal densities and
viabilities thereafter. The mSxl 5 cells typically reached
densities of 1.5-2.5 x 106 cells/mL and exhibited doubling
times of 20 - 30 hours when subcultured every
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant TP Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human RecombinanT 5-HT2B Serotonin Receptor
RECOMMENDED
ASSAY CONDITIONS:
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1 mL aliquots
EX-CELL 420 Serum-Free Medium for Insect Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
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