storage at -80ºC. 106 293T cells were
pelleted and the supernatant (media) was removed. Cell pellets were resuspended in
500 µl of DNAgard or water (non-protected). For comparison, 106 cells were spotted
each well to bring the volume to 50 µL.
Sample Preparation
Tissue samples (10 mg) or cells (1 × 106) can be
homogenized in 200 µL of ice cold GPDH Assay Buffer.
Keep on ice for 10
Preparation
Clear liquid samples may be assayed directly.
Tissue samples (10 mg) or cells (1 × 106) can be
homogenized in 100 µL of ice cold G3P Assay Buffer.
Keep on ice for 10 minutes. Centrifuge
. Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in HEK293 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense 1
mL aliquots into
high-viability culture grown in EX-CELL® CD CHO
Fusion medium in mid-logarithmic growth phase
at 1×106 viable cells/mL. Cells were incubated for
24 hours and sampled to ensure high viability and an
appropriate
procedure and
sampling plan for this study
Process intermediate
2 × 106 TCID50/mL MVM
Fraction 1: Product
Filtration (0–50%
maximum throughput)
Process interruption
for 10 minutes
Fraction
Drying of
Co-amorphous Indomethacin-Arginine With Polymers. Journal of
Pharmaceutical Sciences, 2017. 106(1): p. 302-312.
54. Osama, M., et al., A comparative study of the effect of spray
drying and hot-melt