BALB/c mouse immunized with a synthetic peptide
corresponding to amino acid residues YPYDVPDYA (98-
106) of the human influenza virus hemagglutinin (HA),
conjugated to KLH. The isotype is determined by
for the infectivity of the human
virus.6 The HA tag is a short sequence derived from
amino acids 98-106 of the HA molecule. Many
recombinant proteins have been engineered to express
the HA tag, which
major spike membrane glycoprotein.
The nucleic acid sequence encoding this peptide
(amino acids 98-106 of influenza HA) has been
incorporated into various expression plasmids
adjacent to the cloning
for the infectivity of the human
virus.6 The HA tag is a short sequence derived from
amino acids 98-106 of the HA molecule. Many
recombinant proteins have been engineered to
express the HA tag, which
) polymerase-1 is
required for efficient HIV-1 integration. Proc. Natl.
Acad. Sci. USA, 98, 3364-3368 (2001).
9. Babiychuk, E., et al., Higher plants possess two
structurally different poly(ADP-ribose
infectivity of the virus.6
The HA-Tag consists of a short sequence that
corresponds to amino acids 98-106 of HA. Many
recombinant proteins have been engineered to express
the HA-Tag, which does not appear
application.
Specific Activity: 1 to 1.5 x 106 Units/mg
A unit is defined using rhlL-4 as the reference in the
CTLL-2 cell proliferation assay.
Purity: >98% by SDS-PAGE
Endotoxin level is < 0.1
application.
Specific Activity: 1 to 1.5 x 106 Units/mg
A unit is defined using rhlL-4 as the reference in the
CTLL-2 cell proliferation assay.
Purity: >98% by SDS-PAGE
Endotoxin level is < 0.1
Cell Viability Analyzer or similar device, or
with a hemocytometer.
9. Adjust the volume to give a cell density of 0.9 to 1.1
x 106 viable cells/mL
10. Place the flask in the incubator. CO2 and humidity
the human
virus.6 The short sequence derived from the HA
molecule corresponding to amino acids 98-106 has
been used as a tag, known as the HA-Tag. Many
recombinant proteins have been engineered
CTLL-2
indicator cells.9
Specific Activity: 1 to 1.5 x 106 Units/mg
A Unit is defined using rhlL-2 as the reference in the
CTLL-2 cell proliferation assay.
Purity: >98% by SDS-PAGE
References
.
Performance
When assayed by flow cytometric analysis, using 10 µl
of the antibody per 1 x 106 cells and ExtrAvidin-FITC
Conjugate (Product No. E2761), a fluorescence intensity
is observed similar
CTLL-2
indicator cells.9
Specific Activity: 1 to 1.5 x 106 Units/mg
A Unit is defined using rlL-2 as the reference in the
CTLL-2 cell proliferation assay.
Purity: >98% by SDS-PAGE
References
.
Product Profile
When assayed by flow cytometric analysis, using 10 µl
of the antibody per 1 X 106 cells and ExtrAvidin -FITC
Conjugate (Product No. E 2761), a fluorescence
intensity is observed similar
(95-98%) when cultivated in the EX-CELL® CD Insect
Cell Medium with passaging every 3-4 days. A seven
day growth assay performed on the cells in duplicate
showed day 7 cell densities at 8 x 106 cells