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MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
44 22 30 28 134 26
std
6
20 11 16 15 110 42 4888 95 15 43 25 31 29 134 25
18 11 16 17 107 42 5039 94 13 43 24 31 29 133
The Effective Use of Protein Kinase Inhibitors
66 28 94 79 20 117 64 43 70 93 71 22 90 87 74 27
49 109 21 42 33 84 39 83 106 84 6 6 4 86 113 99 83 60 52 78 107 107
The Effective Use of Protein Kinase Inhibitors
66 28 94 79 20 117 64 43 70 93 71 22 90 87 74 27
49 109 21 42 33 84 39 83 106 84 6 6 4 86 113 99 83 60 52 78 107 107
Quality Control Range Sheet- A6602-107 and A6602-207
, HCYTA-60K-PXBK38, HCYTA-60K-PX48, HCYTMAG-60K-PXBK48
Control Catalog # HCYTA-6060-2 Lot # A6602-107 and A6602-207
***Additional Analytes on reverse side***
Note: The Quality Control Ranges are
Post-Translational Modifications
1-19
K15ac
H2B
107-125
unmod
H2B
107-125
K120ac
H4
7-26
unmod
H4
7-26
SIP
H4
7-26
R3me1
H4
7-26
R3me2a
H4
7-26
R3me2s
Data Sheet - F7751
Mol. Formula: C20H29FO3
Mol. Wt.: 336.45 (anhyd.)
m.p.: 288-290°C
CAS Registry No.: 76-43-7
Chemical Name: 4-Androsten-9α-fluoro-17α-methyl-11β,17β-diol-3-one
Data Sheet - SAB4200173
Mol. Cell
Biol., 2, 107-117 (2001).
5. Lutcke, A., et al., J. Cell Sci., 107, 3437-3448
(1994).
6. Dejgaard, S.Y., et al., J. Cell Sci., 121, 2768-2781
(2008).
7. Ozeki, S.
Data Sheet - C7994
of CacyBP in NB2a cells increases the
level of GAP-43, a marker of differentiation, implying
a potential role of CacyBP in cell differentiation. 5-7
The product is supplied as a solution in 20 mM Tris
Product Information Sheet - B2629
°C
Product Description
Molecular Formula: C5H11NO2
Molecular Weight: 117.1
CAS Number: 107-43-7
pKa: 1.83
1
Melting Point: 310 °C (with decomposition)
Synonyms: oxyneurine, glycine betaine
Data Sheet - R9011
Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood OR
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent. Cells should be >90% viable as
determined by dye exclusion (trypan blue).
MultiScreen Filter Plates with Ultracel®-10 Membrane
142 176 140 136 138 131 123
Cyto c 122 131 124 119 118 123 124 117
FBS 79 70 57 43 42 54 67 72
Human 64 55 43 33 33 43 54 61
30 ABS 68 57 45
AbSurance™ Histone Antibody Specificity Arrays
H2B
1-19
K12ac
H2B
1-19
S14P
H2B
1-19
K15ac
H2B
107-125
unmod
H2B
107-125
Data Sheet - SAB4200809
(1999).
6. Gerdin, E. et al., Am. J. Clin. Pathol., 99, 28-31
(1993).
7. Defize, L.H. et al., J. Cell Biol., 107, 939-49
(1988).
8. Truong, T.H., and Carroll, K.S., Biochemistry,
51
Data Sheet - A5691
Differentiation, 43, 115
(1990).
6 Jahoda, C., et al., J. Cell Sci., 99, 627 (1991).
7. Sappino, A., et al., Lab. Invest., 63, 144 (1990).
8. Ruzicka, D., et al., J. Cell Biol., 107, 2575 (1988).
Data Sheet - F7027
Immunol., 43, 49 (1981).
Fischer, A., et al., Immunol., 48, 177 (1983).
Furley, A., et al., Cell, 46, 75 (1986).
Garson, J., et al., Nature, 298, 375 (1982).
Glazer, N., et al., J. Biophys. Soc., 43, 383
Data Sheet - SAB4200729
587, 1220-9
(2013).
6. Belkin AM., et al., J Biol Chem., 263, 6631-5
(1988).
7. Belkin AM., J Cell Biol., 107, 545-53 (1988).
8. Westmeyer A., et al., EMBO J., 9, 2071-8
(1990).
Data Sheet - SAB4200670
Motil., 9, 30-47
(1988).
5. Harris A., et al., Development, 107, 771-784 (1989).
6. Morkin E., Microsc Res Tech., 50, 522-531 (2000).
7. Hoh, J., et al., J. Muscle Res. Cell Motil., 9, 48-58
(1988
Product Information Sheet - A3306
thesis, p. 43
(1992).
19. Kotchan, Andrew Brian, "Evaluation of Yucca
Extract, Amino Acid and Jerusalem Artichoke
Supplementation in Pig Diets". University of
Manitoba, M.Sc. thesis, p. 43 (1998)
Product Information Sheet - I1149
,
1967.
50. Lundblad, R.L., Techniques in Protein Modification,
CRC Press, London, 1995, 107.
51. Gurd, F.R.N., Methods Enzymol., 25, 424, 1972.
52. Nishita, T. and Deutsch, H.F., Biochem.
Product Information Sheet - I6125
,
1967.
50. Lundblad, R.L., Techniques in Protein Modification,
CRC Press, London, 1995, 107.
51. Gurd, F.R.N., Methods Enzymol., 25, 424, 1972.
52. Nishita, T. and Deutsch, H.F., Biochem.
Data Sheet - F3777
Differentiation, 43, 115
(1990).
6. Jahoda, C., et al., J. Cell Sci., 99, 627 (1991).
7. Sappino, A., et al., Lab. Invest., 63, 144 (1990).
8. Ruzicka, D., and Schwartz, R., J. Cell Biol.
Acrylamido Buffers
209 193 842 - - 915 184 372 16
Data Sheet - C6198
Differentiation, 43, 115
(1990).
6. Jahoda, C., et al., J. Cell Sci., 99, 627 (1991).
7. Sappino, A., et al., Lab. Invest., 63, 144 (1990).
8. Ruzicka, D., and Schwartz, R., J. Cell Biol., 107,
2575
Product Information Sheet - N6505
and Co. (New York, NY), p, 564 (2002).
3. Branchaud, B.P., and Walsh, C.T., J. Am. Chem.
Soc., 107(7), 2153-2161 (1985).
4. Gintes, Marie-Line, "Health Assessment of Tree
Swallows (Tachycineta bicolor
Product Information Sheet-11814770001
Solution
[μl]
Protein
Precipitation
Solution [ml] *)
1 × 107 3 2 100 1.2
2 × 107 6 4 200 2.5
3 × 107 9 6 300 3.7
4 × 107 12 8 400 5
*) Calculate the volume of
Data Sheet - F7152
Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent. Cells should be >90% viable as deter-
mined by dye exclusion (e.g., trypan blue
LABORATORY NOTEBOOK ISSUANCE PAGE
15
1.
96
15
7.
25
23
2.
04
23
1.
04
23
8.
03
(2
37
)
(2
44
)
(2
43
)
(2
47
)
58
.6
93
63
.5
46
65
.3
8
Product Information Sheet - S6576
-chip assays.
Biotechniques, 41:577-580 (2006).
7. Acevedo LG, et al., Genome-scale ChIP-chip
analysis using 10,000 human cells. Biotechniques,
43:791-797 (2007).
8. O’Geen, H., et al., Genome-Wide
LABORATORY NOTEBOOK ISSUANCE PAGE
15
1.
96
15
7.
25
23
2.
04
23
1.
04
23
8.
03
(2
37
)
(2
44
)
(2
43
)
(2
47
)
58
.6
93
63
.5
46
65
.3
8
LABORATORY NOTEBOOK ISSUANCE PAGE
15
1.
96
15
7.
25
23
2.
04
23
1.
04
23
8.
03
(2
37
)
(2
44
)
(2
43
)
(2
47
)
58
.6
93
63
.5
46
65
.3
8
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