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Claudia Gandolfo et al.
Vaccines, 10(2) (2022-02-27)
We have designed a prospective study aiming to monitor the immune response in 178 health care workers six months after BNT162b2 mRNA vaccination. The humoral immune response of all subjects was evaluated by chemiluminescence (CMIA); in 60 serum samples, a
J L Ceuppens et al.
Journal of immunology (Baltimore, Md. : 1950), 141(11), 3868-3874 (1988-12-01)
T cell proliferation induced by the lectin PHA requires the presence of monocytes. Here, we report that IL-6 represents part of the monocyte-derived helper activity for human T cell stimulation with PHA. Resting T cells isolated from peripheral blood or
Tamara Muliaditan et al.
Cell reports. Medicine, 2(12), 100457-100457 (2022-01-15)
Second generation (2G) chimeric antigen receptors (CARs) contain a CD28 or 41BB co-stimulatory endodomain and elicit remarkable efficacy in hematological malignancies. Third generation (3G) CARs extend this linear blueprint by fusing both co-stimulatory units in series. However, clinical impact has
Lijun Cong et al.
mBio, 12(4), e0192021-e0192021 (2021-08-25)
Human immunodeficiency virus (HIV) remodels the cell surface of infected cells to facilitate viral dissemination and promote immune evasion. The membrane-associated viral protein U (Vpu) accessory protein encoded by HIV-1 plays a key role in this process by altering cell
Johannes Nowatzky et al.
Journal of visualized experiments : JoVE, (159) (2020-06-02)
Human regulatory T cells (Treg) are notoriously difficult to isolate in high purity given the current methods of Treg enrichment. These methods are based on the identification of Treg through several activation-dependent cellular surface markers with varying expression levels in
Rebecca P Payne et al.
Cell, 184(23), 5699-5714 (2021-11-05)
Extension of the interval between vaccine doses for the BNT162b2 mRNA vaccine was introduced in the United Kingdom to accelerate population coverage with a single dose. At this time, trial data were lacking, and we addressed this in a study
An optimized IFN-γ ELISpot assay for the sensitive and standardized monitoring of CMV protein-reactive effector cells of cell-mediated immunity.
Barabas S, et al.
BMC Immunology, 18(1), 14-14 (2017)
ELISPOT Assay to Measure Peptide-specific IFN-γ Production.
Wykes M N and Renia L.
Bio-protocol, 7(11) (2017)
Torkild Visnes et al.
Nucleic acids research, 48(21), 12234-12251 (2020-11-20)
Altered oncogene expression in cancer cells causes loss of redox homeostasis resulting in oxidative DNA damage, e.g. 8-oxoguanine (8-oxoG), repaired by base excision repair (BER). PARP1 coordinates BER and relies on the upstream 8-oxoguanine-DNA glycosylase (OGG1) to recognise and excise
Michelle N Wykes et al.
Bio-protocol, 7(11), e2302-e2302 (2017-06-05)
Interferon-gamma (IFN-γ) is crucial for immunity against intracellular pathogens and for tumor control. It is produced predominantly by natural killer (NK) and natural killer T cells (NKT) as well as by antigen-specific Th1 CD4+ and CD8+ effector T cells. When
Max Baymiller et al.
The Journal of biological chemistry, 298(4), 101757-101757 (2022-02-25)
The aminoacyl-tRNA synthetases are an ancient and ubiquitous component of all life. Many eukaryotic synthetases balance their essential function, preparing aminoacyl-tRNA for use in mRNA translation, with diverse roles in cell signaling. Herein, we use long-read sequencing to discover a
Abolfazl Movafagh et al.
Iranian journal of pharmaceutical research : IJPR, 10(4), 895-903 (2011-10-01)
Phytohemagglutinin (PHA) is a lectin, obtained from the red kidney bean that binds to the membranes of T-cells and stimulates metabolic activity, cell division, etc. The object of this research was the comparison between self made PHA (Indigenous) and imported
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