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Hiroshi Yokoyama et al.
Journal of bacteriology, 196(21), 3784-3792 (2014-08-27)
Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that
Nadav Sorek et al.
The Journal of biological chemistry, 289(50), 34911-34920 (2014-10-22)
Mutations in the Arabidopsis COBRA gene lead to defects in cellulose synthesis but the function of COBRA is unknown. Here we present evidence that COBRA localizes to discrete particles in the plasma membrane and is sensitive to inhibitors of cellulose
Alexander V Gusakov
Science (New York, N.Y.), 344(6184), 578-578 (2014-05-09)
Brunecky et al. (Reports, 20 December 2013, p. 1513) compared the cellulolytic activity of bacterial multimodular cellulase CelA with fungal Cel7A (cellobiohydrolase I from Trichoderma reesei). If more active Cel7A from another fungus were used as a reference enzyme under
Jeffrey M Skerker et al.
Molecular systems biology, 9, 674-674 (2013-06-19)
The efficient production of biofuels from cellulosic feedstocks will require the efficient fermentation of the sugars in hydrolyzed plant material. Unfortunately, plant hydrolysates also contain many compounds that inhibit microbial growth and fermentation. We used DNA-barcoded mutant libraries to identify
Manuel Eibinger et al.
The FEBS journal, 281(1), 275-290 (2013-12-11)
Highly heterogeneous and usually weakly defined substrate morphologies complicate the study of enzymatic cellulose hydrolysis. The cellulose surface has a non-uniform shape in particular, with consequent impacts on cellulase adsorption and activity. We have therefore prepared a cellulosic model substrate
Hernan Charreau et al.
Recent patents on nanotechnology, 7(1), 56-80 (2012-07-04)
Cellulose nanoparticles (i.e. cellulose elements having at least one dimension in the 1-100 nm range) have received increasing attention during the last decade. This is not only evident in academic articles, but it is also manifested by the increasing number
Zhiyu Shu et al.
Biochemistry, 53(48), 7650-7658 (2014-11-26)
One important feature of hydrolysis of cellulose by cellulases is that the reaction slows down quickly after it starts. In this work, we investigate the slowdown mechanism at the early stage of the reaction using endoglucanase Tr. Cel5A-catalyzed phosphate acid-swollen
Combining computational chemistry and crystallography for a better understanding of the structure of cellulose.
Alfred D French
Advances in carbohydrate chemistry and biochemistry, 67, 19-93 (2012-07-17)
Battle for the barrel.
Robert F Service
Science (New York, N.Y.), 339(6126), 1374-1377 (2013-03-23)
Anne-Catherine Servais et al.
Journal of chromatography. A, 1363, 178-182 (2014-07-19)
The ophthalmic solution of voriconazole, i.e. (2R,3S)-2-(2,4-difluorophenyl)-3-(5-fluoropyrimidin-4-yl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol, made from an injection formulation which also contains sulfobutylether-β-cyclodextrin sodium salt as an excipient (Vfend), is used for the treatment of fungal keratitis. A liquid chromatographic (LC) method using polar organic mobile phase
Scott Wushke et al.
Canadian journal of microbiology, 59(10), 679-683 (2013-10-10)
Ethanol production from direct cellulose fermentation has mainly been described as a strictly anaerobic process. The use of air-tolerant organisms or consortia for this process would reduce the need for prereduction of the medium and also permit continuous feed of
Rong Huang et al.
Journal of biomedical nanotechnology, 10(7), 1346-1358 (2014-05-09)
Catalase, a kind of redox enzyme and generally recognized as an efficient agent for protecting cells against hydrogen peroxide (H2O2)-induced cytotoxicity. The immobilization of catalase was accomplished by depositing the positively charged chitosan and the negatively charged catalase on electrospun
Minh Hong Nguyen et al.
Journal of bioscience and bioengineering, 118(4), 400-405 (2014-04-22)
Polysaccharides are major structural constituents to develop the three-dimensional architecture of Escherichia coli biofilms. In this study, confocal laser scanning microscopy was applied in combination with a fluorescent probe to analyze the location and arrangement of exopolysaccharide (EPSh) in microcolonies
Jingquan Han et al.
Carbohydrate polymers, 94(2), 773-781 (2013-04-03)
Regenerated cellulose nanoparticles (RCNs) including both elongated fiber and spherical structures were prepared from microcrystalline cellulose (MCC) and cotton using 1-butyl-3-methylimidazolium chloride followed by high-pressure homogenization. The crystalline structure of RCNs was cellulose II in contrast to the cellulose I
Chun You et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(18), 7182-7187 (2013-04-17)
The global demand for food could double in another 40 y owing to growth in the population and food consumption per capita. To meet the world's future food and sustainability needs for biofuels and renewable materials, the production of starch-rich
Kornelia Batko et al.
Polimery w medycynie, 43(1), 11-19 (2013-07-03)
The reflection coefficient of the membrane (sigma) is one of the basic parameters of the polymer membrane transport. Classical methods used to determine this parameter require intensive mixing of two solutions separated by a membrane to eliminate the effects of
Heather E McFarlane et al.
Annual review of plant biology, 65, 69-94 (2014-03-04)
Plant stature and development are governed by cell proliferation and directed cell growth. These parameters are determined largely by cell wall characteristics. Cellulose microfibrils, composed of hydrogen-bonded β-1,4 glucans, are key components for anisotropic growth in plants. Cellulose is synthesized
Lorena M Herrera et al.
Microbiological research, 223-225, 13-21 (2019-06-11)
Flavobacterium sp. AUG42 is a cellulase-producing bacterium isolated from the Antarctic oligochaete Grania sp. (Annelida). In this work, we report that AUG42 produces a glycoside hydrolase cocktail with CMCase, PASCase and cellobiase activities (optimum pHs and temperatures ranging from 5.5
Orly Salama-Alber et al.
The Journal of biological chemistry, 288(23), 16827-16838 (2013-04-13)
The rumen bacterium Ruminococcus flavefaciens produces a highly organized multienzyme cellulosome complex that plays a key role in the degradation of plant cell wall polysaccharides, notably cellulose. The R. flavefaciens cellulosomal system is anchored to the bacterial cell wall through
Chang Kee Lee et al.
Journal of nanoscience and nanotechnology, 13(11), 7391-7395 (2013-11-20)
We have reported the fabrication of flexible graphene-paper electrode (GPE) with a flat surface, whose internal structure has been formed with gradient porous build-up (from the surface to the 2-hydroxyethyl cellulose (HC)-coated paper). HC solution was used as a binder
Edmond Lam et al.
Trends in biotechnology, 30(5), 283-290 (2012-03-13)
Nanocrystalline cellulose (NCC), a rod-shaped nanoscale material with exceptional strength and physicochemical properties, can be prepared from inexpensive renewable biomass. Besides its potential use as a reinforcing agent for industrial biocomposites, pristine NCC exhibits low toxicity and poses no serious
Daniel L Warner et al.
Environmental monitoring and assessment, 187(1), 4208-4208 (2014-12-17)
Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints. Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium
Hui Wang et al.
Chemical Society reviews, 41(4), 1519-1537 (2012-01-24)
Utilization of natural polymers has attracted increasing attention because of the consumption and over-exploitation of non-renewable resources, such as coal and oil. The development of green processing of cellulose, the most abundant biorenewable material on Earth, is urgent from the
H P S Abdul Khalil et al.
Carbohydrate polymers, 99, 649-665 (2013-11-28)
Nanofibrillated cellulose from biomass has recently gained attention owing to their biodegradable nature, low density, high mechanical properties, economic value and renewability. Although they still suffer from two major drawbacks. The first challenge is the exploration of raw materials and
Yi Zheng et al.
Materials science & engineering. C, Materials for biological applications, 33(4), 2407-2412 (2013-03-19)
Flexible magnetic membrane based on bacterial cellulose (BC) was successfully prepared by in-situ synthesis of the Fe3O4 nanoparticles under different conditions and its properties were characterized. The results demonstrated that the Fe3O4 nanoparticles coated with PEG were well homogeneously dispersed
Victor R Gordeuk et al.
American journal of hematology, 95(11), 1269-1279 (2020-07-23)
Genetic modifiers of anemia in Plasmodium falciparum infection and sickle cell disease (SCD) are not fully known. Both conditions are associated with oxidative stress, hemolysis and anemia. The CYB5R3 gene encodes cytochrome b5 reductase 3, which converts methemoglobin to hemoglobin
Xiaoxu Song et al.
Ultrasonics, 54(7), 2042-2049 (2014-06-18)
Temperature in ultrasonic vibration-assisted (UV-A) pelleting of cellulosic biomass has a significant impact on pellet quality. However, there are no reports on temperature models for UV-A pelleting of cellulosic biomass. The development of a physics-based temperature model can help to
Dorottya Laczkó et al.
Immunity, 53(4), 724-732 (2020-08-14)
SARS-CoV-2 infection has emerged as a serious global pandemic. Because of the high transmissibility of the virus and the high rate of morbidity and mortality associated with COVID-19, developing effective and safe vaccines is a top research priority. Here, we
Aja Aravamudhan et al.
Journal of biomedical nanotechnology, 9(4), 719-731 (2013-04-30)
Scaffold based bone tissue engineering (BTE) has made great progress in regenerating lost bone tissue. Materials of natural and synthetic origin have been used for scaffold fabrication. Scaffolds derived from natural polymers offer greater bioactivity and biocompatibility with mammalian tissues
Daliana Muller et al.
Carbohydrate polymers, 94(1), 655-662 (2013-04-03)
An electrically conducting composite based on bacterial cellulose (BC) and polypyrrole (PPy) was prepared through in situ oxidative polymerization of pyrrole (Py) in the presence of BC membrane using ammonium persulfate (APS), as an oxidant. The electrical conductivity, morphology, mechanical
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