solutions at the following concentra-
add 5.0 ml of 0.01 M sodium phosphate buffered tions: 500, 250, 125, 63, 31, and 15 pg/0.1 ml.
saline, pH 7.4, containing 0.1% BSA and 0.1% (B) Dilution buffer: 0.01 M phosphate
Neutralization Dose50 (ND50) for Anti-FGF-7 is
~ 6-12 µg/mL in the presence of 125 ng/mL of
recombinant human FGF-7 using the 4MBr-5 cell line.
The ND50 is the concentration of antibody required to
further diluted in
normal human serum to obtain standard solutions at
the following concentrations: 125, 63, 31 and 15
(B) Phosphate Buffered Saline, 0.01M, pH 7.4, contain-
ing 0.1% sodium azide
Phosphatase Assay Buffer – 125 mM HEPES, pH7.2,
250 mM NaCl, and 12.5 mM EDTA.
Phosphatase Dilution Buffer – Dilute the Phosphatase
Assay Buffer 5-fold with a 5 mM DTT and 65 ng/µl BSA
further diluted in buffer (B)
to obtain standard solutions at the following
concentrations: 125, 63, 31 and 15 pg/0.1 mL.
(B) Dilution buffer: 0.05 M Tris-HCl (Catalog Number
T3253), pH 8.0, containing
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