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To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

Poly(N-isopropylacrylamide)-based Smart Surfaces for Cell Sheet Tissue Engineering

Tissue engineering has become a key therapeutic tool in the treatment of damaged or diseased organs and tissues, such as blood vessels and urinary bladders.

Order shRNA Online in 5 Easy Steps

5 easy steps to order shRNA gene of your interest.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

Analysis of Pesticides and their Metabolite Residues

Pesticide residue analysis of 331 pesticides and 44 metabolites in cabbage samples using Supel™ QuE QuEChERS mixes and Purospher® STAR RP-18e column.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Download our Training Program

Our Training Program is availabale in 5 different languages.

Puriﬁcation using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Hydroxymethylfurfural Analysis of Beer by HPLC UV

HPLC-UV determination of 5-HMF (5-(hydroxymethyl)furfural) in beer samples using monolithic silica Chromolith® HighResolution RP-18e HPLC column.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature

Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.

Reflectometric Determination of Hydroxymethylfurfural in Honey

Rapid analysis of 5-(Hydroxymethyl)furfural (HMF) in honey using a reflectometer and Reflectoquant® HMF Test.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Critical Inhibitor Characteristics to Consider

Discover critical characteristics to consider when working with enzyme inhibitors, such as cell permeability, the prozone effect, and Lipinski’s rule of 5.

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Block Copolymers in Nanoscale Patterning

Block copolymers coat surfaces for nanoscale patterns, improving resolution to 5-50nm, enabling diverse applications like printable circuits.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Integrity Testers

Efficient Filter Integrity Testing: Elevate quality control with Integritest® 5, offering bubble point, diffusion, and hydrophobic testing in-line or offline.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

QuEChERS Method for Pesticide Residue Analysis

QuEChERS method for pesticide residue analysis requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Determination of Water Content in Phenol Using Karl Fischer Titration

Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.

Critical Micelle Concentrations (CMCs) - Avanti® Polar Lipids

The critical micelle concentration (CMC) can be approximately defined as the lipid monomer concentration at which appreciable amounts (>5% of total) of micellar aggregates first begin to appear in the equilibrium: nM1Mn

Non-Fat Dry Milk (NFDM) Solution for Blocking Western Blots

Non-Fat Dry milk (NFDM) buffered solution is ideal for saturating excess protein-binding sites on membranes for Western blotting. Typically, 5% NFDM is sufficient for most Western blots.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Purification of NAD+ and ATP-dependent Kinases

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

Detection of Signaling Effector-Complexes Downstream of BMP4 Using In Situ PLA, A Proximity Ligation Assay

BMPs are responsible for a wide range of developmental and biological effects. BMP receptors activate (phosphorylate) the Smad1/5/8 effectors, which then, form a complex with Smad4 and translocate to the nucleus where they function as transcription factors to initiate BMP

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