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RNA Oligo Specifications, Shipping Schedule, Minimum Yield Guarantees, Quality Control, Quality Assurance

SeQuant® HILIC (hydrophilic interaction liquid chromatography) HPLC columns constitute a range of high-performance tools for separating polar hydrophilic compounds.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

Purospher™ STAR HPLC and UHPLC Columns

For outstanding separations with excellent peak symmetry, our range of Purospher™ STAR HPLC and UHPLC columns are available with a variety of modifications to suit your selectivity needs.

Chromatography Column Sizing Chart

Flash-Chromatography columns are available with ball joints or threaded standard taper joints and EZSafe™ connections and are useful for rapid, preparative separations. Large columns are also available with Ace-Thred connectors.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

Cell Harvest, Lysis, Neutralization and Clarification of Plasmid DNA (pDNA) for mRNA, plasmid-based DNA Vaccines, and Viral Vector applications

An overview of the cell harvest, lysis, neutralization, and clarification steps used for pDNA purification along with case study and practical advice.

Cell-based Vaccine Manufacturing Processes for Creating Attenuated Viral Vaccines

Attenuated viral vaccines are created through passage and harvesting from foreign cells or hosts. This technical article describes the manufacturing process of these viral vaccines.

Custom DNA Oligos: Standard DNA Synthesis

High-quality custom DNA oligos made to meet your specifications (scale, purification, length, dry/solution, modifications, tubes/plates).

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

PCR/qPCR/dPCR Assay Design

The entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and

Working with BRAND® Liquid Handling Instruments

This page covers all essential information regarding working with liquid handling instruments, including dispensers, burettes, and pipettes.

GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on Equity®-1

Protocol for GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on Equity®-1

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Egg-based and Cell-based Influenza Vaccine Manufacturing Methods

Influenza vaccines are commonly made using egg-based and cell-based manufacturing strategies. Find step-by-step information on the manufacturing process for each method.

Cyclodextrin Solubility Table

Solubility of common cell culture components in water and in a solution of 2-Hydroxylpropyl beta-cyclodextrin. Cyclodextrin is a non-toxic compound useful for its ability to solubilize fat soluble vitamins and hormones.

US EPA Method 8015 (modified): GC Analysis of Gasoline Range Organics (GRO) on SLB®-5ms, Direct Injection

Determination of PAHs in Paprika Powder Using a Novel SPE Cartridge

Paprika is a spice made from dried sweet peppers, and is used for flavor and color in many types of cuisine. Contamination with polynuclear aromatic hydrocarbons (PAHs) can occur when pepper plants are exposed to these pollutants in the environment

Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

HPLC Analysis of PEG g006200

HPLC Analysis of PEG on Two Zenix® SEC-300 Columns in Tandem

Carbon Nanofiber Applications & Properties

Pyrograf®-III vapor-grown carbon nanofibers are within the class of materials termed multi-walled carbon nanotubes (MWCNTs), and are produced by the floating catalyst method.

Water Determination in Liquefied Petroleum Gas using GC BID and Ionic Liquid Column Watercol™

Using Watercol™ GC columns in conjunction with GC-BID can provide a reliable and practical option for Water determination in petrochemical feedstocks.

Water Determination in Liquefied Petroleum Gas using GC BID and Ionic Liquid Column Watercol^™

Using Watercol™ GC columns in conjunction with GC-BID can provide a reliable and practical option for Water determination in petrochemical feedstocks.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Rugged and Reproducible Solid Phase Microextraction (SPME) Sampling

SPME fibers with Carboxen®/Polydimethylsiloxane (CAR/PDMS) and Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) coatings are now available on a nitinol core (NIT) in a SS assembly for improved fiber performance.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

Dispensette® Testing Instructions (SOP)

This article provides testing instructions for BRAND Dispensette® S bottle-top liquid dispenser according to the standard ISO DIS 8655.

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