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Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

Performing a Separation with IgG Sepharose 6 Fast Flow

Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Oligonucleotide Standard 6 Mix LC-UV Analysis

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Dextran

Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.

Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Removal of Albumin Using Blue Sepharose® Chromatography Media

Remove albumin from afﬁnity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.

Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.

Purification or Removal of DNA-Binding Proteins

This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.

USDA FSIS STEC Guidance Implementation

Discover the expanded USDA FSIS verification testing for the 'Big 6' non-O157 STEC in beef products and explore accurate testing solutions and industry practices for enhanced food safety.

Determination of Water Content in Phenol Using Karl Fischer Titration

Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.

Preservation of Moisture-Sensitive Chemical Reagents

Preserve reagent quality of air- and moisture-sensitive reagents using nitrogen or argon in crown-cap bottles with a 6 mm diameter hole in the crown-cap and a PTFE-faced rubber liner.

Puriﬁcation of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin

This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.

Purification of NAD+ and ATP-dependent Kinases

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

Formulation and Delivery US 2024

Join us at the Formulation and Delivery US conference at booth #6 to learn about our integrated offering for all process steps in pharmaceutical and biopharmaceutical manufacturing which includes products that meet the highest quality and purity standards with extensive

DNAstable® Protocol

DNAstable is a unique storage medium that preserves genomic DNA, plasmids, bacterial artificial chromosomes (BACs), PCR products and oligonucleotides at room temperature.

Column Packing and Preparation for Size Exclusion Chromatography

Biodegradable Aliphatic Polyesters for Drug Delivery

Innovations in polymer technology have had a significant impact on the advancement of novel drug delivery systems.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Suero Group – Professor Product Portal

The Suero research group has developed new catalytic methods involving conceptually-novel radical carbenoids, carbyne equivalents, and metal-carbynoids and studied their behavior towards fine & feedstock chemicals as well as medically relevant agents.

Selective Media for Bifidobacteria

Bifidobacteria are important indicators of quality control in the manufacture of dairy products. Bifidobacteria Selective Media (BSM) allows for easy and fast quality control of yoghurt.

Chitosan-based Biomaterials

Chitin, a natural polysaccharide, is the second most abundant natural biopolymer in the world, after cellulose.

Determination of Stevioside and Rebaudioside A in Stevia leaves, beverages and food additives using HPTLC-MS

HPTLC-MS was used to analyze stevioside and rebaudioside in artificial sweeteners, stevia plants, cola and isotonic drinks with a minimum of sample preparation.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

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