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18373

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Shangguo Hou et al.
Nature communications, 11(1), 3607-3607 (2020-07-19)
To date, single molecule studies have been reliant on tethering or confinement to achieve long duration and high temporal resolution measurements. Here, we present a 3D single-molecule active real-time tracking method (3D-SMART) which is capable of locking on to single
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B., et al.
Optical Nanoscopy, 1, 3-3 (2012)
An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
Vicidomini, G.; et al.
Nano Letters, 15(9), 5912-5918 (2015)
Cornelia Hunke et al.
Journal of bioenergetics and biomembranes, 42(1), 1-10 (2010-01-19)
Subunit alpha of the Escherichia coli F(1)F(O) ATP synthase has been produced, and its low-resolution structure has been determined. The monodispersity of alpha allowed the studies of nucleotide-binding and inhibitory effect of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) to ATP/ADP-binding. Binding constants (K (
Jennifer Z Yao et al.
Journal of the American Chemical Society, 134(8), 3720-3728 (2012-01-14)
Methods for targeting of small molecules to cellular proteins can allow imaging with fluorophores that are smaller, brighter, and more photostable than fluorescent proteins. Previously, we reported targeting of the blue fluorophore coumarin to cellular proteins fused to a 13-amino
STED Microscopy to Monitor Agglomeration of Silica Particles Inside A549 Cells.
Schubbe, S.; Cavelius, C.
Advanced Energy Materials, 12, 417-422 (2010)
Enhancing stimulated emission-based fluorescence detection with interferometric setup.
Chung, S.-S.M.; Deng, J.-H.
Proceedings of SPIE, 8948, 89481-89481 (2014)
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope.
Meyer, S. A.; Ozbay, B.
Proceedings of SPIE, 8950, 89500-89500 (2014)
Annedore Punge et al.
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100
STED microscope with Spiral Phase Contrast.
Lauterbach, M. A.; et al.
Scientific Reports, 3 (2013)
Far-field optical nanoscopy based on continuous wave laser stimulated emission depletion.
Kuang, C.; Zhao, W.; Wang, G.; et al.
The Review of Scientific Instruments, 81(5), 53709-53709 (2010)
SNARE Function Is Not Involved in Early Endosome Docking.
Geumann, U.; et al.
Molecular Biology of the Cell, 19(12), 5327-5337 (2008)
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.
Fitzpatrick, JA.; et al.
Bioconjugate Chemistry, 20(10), 1843-1847 (2009)
Quantification of Internalized Silica Nanoparticles via STED Microscopy.
Peuschel, H.; et al.
BioMed Research International, 2015(1), 1-16 (2015)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
Marisa L Martin-Fernandez et al.
International journal of molecular sciences, 13(11), 14742-14765 (2012-12-04)
Insights from single-molecule tracking in mammalian cells have the potential to greatly contribute to our understanding of the dynamic behavior of many protein families and networks which are key therapeutic targets of the pharmaceutical industry. This is particularly so at
Munc18-1 Tuning of Vesicle Merger and Fusion Pore Properties.
Jorgacevski, J.; et al.
The Journal of Neuroscience, 31(24), 9055-9066 (2011)
Volker Westphal et al.
Science (New York, N.Y.), 320(5873), 246-249 (2008-02-23)
We present video-rate (28 frames per second) far-field optical imaging with a focal spot size of 62 nanometers in living cells. Fluorescently labeled synaptic vesicles inside the axons of cultured neurons were recorded with stimulated emission depletion (STED) microscopy in
Exploring single-molecule dynamics with fluorescence nanoscopy.
Ringemann, Ch.; et al
New Journal of Physics, 11(10), 103054-103054 (2009)
Christian B Gerhold et al.
Nucleic acids research, 40(21), 11036-11046 (2012-09-15)
Nuclear actin-related proteins (Arps) are subunits of several chromatin remodelers, but their molecular functions within these complexes are unclear. We report the crystal structure of the INO80 complex subunit Arp8 in its ATP-bound form. Human Arp8 has several insertions in
STED microscopy to monitor agglomeration of silica particles inside A549 cells.
Schubbe, S., et al.
Advanced Engineering Materials, 12, 417-422 (2010)
Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells.
Stagge, F.; et al.
PLoS ONE, 8(10), e78745-e78745 (2013)
Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95.
Nair D.; et al.
The Journal of Neuroscience, 33(32), 13204-13224 (2013)
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Harpreet Singh et al.
Mitochondrion, 12(2), 230-236 (2011-10-11)
The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution
Marina I Giannotti et al.
Biomacromolecules, 12(7), 2524-2533 (2011-05-25)
Nanopharmaceutics composed of a carrier and a protein have the potential to improve the activity of therapeutical proteins. Therapy for lysosomal diseases is limited by the lack of effective protein delivery systems that allow the controlled release of specific proteins
STED nanoscopy. A glimpse into the future.
Bianchini, P.; et al.
Cell and Tissue Research, 360(1), 143-150 (2015)
Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye.
Luo, D., et al.
Optics & Laser technology, 45, 723-725 (2013)
Jialei Tang et al.
Scientific reports, 7(1), 10945-10945 (2017-09-10)
We report a simple single-molecule fluorescence imaging method that increases the temporal resolution of any type of array detector by >5-fold with full field-of-view. We spread single-molecule spots to adjacent pixels by rotating a mirror in the detection path during
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