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2.7.7.7
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Data Sheet - D9380
carrying bacteriophage
Product Number D9380
Storage Temperature −20 °C
CAS# 9012-90-2
EC 2.7.7.7
Synonym: Kornberg polymerase
Product Description
Molecular weight: 109 kDa
DNA polymerase
Product Information Sheet - DNAPOL-RO
After electrophoresis, there is no detectable loss of the supercoiled form observed.
EC-Number
EC 2.7.7.7
Specific Activity
At least 8,000 U/mg (+37°C, poly[d(A-T)] as template).
Unit Definition
One
Metabolic Pathways
2.4.2.4
2.1.1.45
RP
CH
C
O
CHOC N
HN
1.3.1.14 2.4.2.10 4.1.1.23
2.7.7.7
2.7.7.7
2.7.7.7
1.17.4.1
2.7.
7.7
2.7.4.14
4.4.1.1
1.6.4.1
1.13.11.20
4.4.1.8
Product Information Sheet-elong-ro
Version: 26 Expand Long Template PCR System
8 sigma-aldrich.com
2.3. Parameters
EC-Number
EC 2.7.7.7
Error Rate
Threefold more accurate compared to Taq DNA polymerase.
Relative fidelity determined
Product Information Sheet-taq-ro
Thermus aquaticus BM, recombinant (E. coli)
8 sigma-aldrich.com
2.3. Parameters
EC-Number
EC 2.7.7.7
Incorporation of Modified Nucleotides
Enzyme accepts modified nucleotides such as radiolabeled
Product Information Sheet - 11811002001
Expand 20 kbPLUS PCR System
Deoxynucleoside-triphosphate: DNA deoxynucleotidyltransferase, E.C. 2.7.7.7
Cat. No. 11 811 002 001 200 U for approximately 40 reactions y Version 15
Content version: October
Product Information Sheet - PWOPOLRO
100.
From Pyrococcus woesei
Deoxynucleoside-triphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7
y Version 16
Cat. No. 11 644 947 001 100 units Content version: May 2019
Cat. No. 11 644 955
Product Information Sheet-elongn-ro
Version: 09Expand Long Range dNTPack
sigma-aldrich.com 9
2.3. Parameters
EC-Number
EC 2.7.7.7
Error Rate
Approximately threefold higher fidelity of DNA synthesis compared to Taq DNA polymerase
Product Information Sheet-ehifi-ro
Version: 22 Expand High Fidelity PCR System
8 sigma-aldrich.com
2.3. Parameters
EC-Number
EC 2.7.7.7
Error Rate
Threefold more accurate compared to Taq DNA polymerase.
Relative fidelity determined
Product Information Sheet-pwopol-ro
Cycle
number 13 is 15 seconds longer than cycle 10, etc.
2.3. Parameters
EC-Number
EC 2.7.7.7
1
2
3
4
https://www.sigmaaldrich.com
2.
Product Information Sheet-03734927001
recombinant (E. coli)
sigma-aldrich.com 7
2.3. Parameters
Bioburden
≤50 cfu/ml
EC-Number
EC 2.7.7.7
Incorporation of Modified Nucleotides
DIG-dUTP, Biotin-dUTP, etc.
Maximum Fragment Size
Amplifies
Product Information Sheet-taql-ro
Thermus aquaticus BM, recombinant (E. coli)
8 sigma-aldrich.com
2.3. Parameters
EC-Number
EC 2.7.7.7
Incorporation of Modified Nucleotides
Enzyme accepts modified nucleotides such as radiolabeled
Product Information Sheet-ehifip-ro
10Expand High FidelityPLUS PCR System
sigma-aldrich.com 9
2.3. Parameters
EC-Number
EC 2.7.7.7
Error Rate
• Twofold more accurate compared to the Expand High Fidelity PCR System.
• Sixfold
Product Information Sheet-pwosup-ro
, cycle 16 is 50 seconds, cycle
17 is 55 seconds, etc.
2.3. Parameters
EC-Number
EC 2.7.7.7
Error Rate
Pwo SuperYield DNA Polymerase has approximately 18-fold higher fidelity of DNA synthesis
Product Information Sheet-11480022001
depends on the abundance (copy number) of the respective RNA.
2.3. Parameters
EC-Number
EC 2.7.7.7
Incorporation of Modified Nucleotides
Accepts modified deoxyribonucleoside triphosphates as substrates
