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Refolding of proteins using gel ﬁltration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution puriﬁcation of GST-tagged proteins, providing sharp peaks and concentrated eluent.

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Virus Concentration by Ultrafiltration

Ultrafiltration using Amicon® Ultra centrifugal devices for concentrating virus in preparation of high titer virus stocks and isolation of virus from samples.

Materials for Clean H² Production from Bioethanol Reforming

Hydrogen is one of the most important resources in providing food, fuel, and chemical products for our everyday life. Sustainable catalytic hydrogen production from bioethanol has gained significant attention in recent years due to globally diminishing fossil fuel supplies, which

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt

Validation of a Simple Method for the Alcohol Content in Kombucha Tea by Headspace SPME and GC-MS

Development and validation of a headspace SPME and GC-MS method to determine alcohol content in twenty different Kombucha tea samples.

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.

Corning® Spheroid Microplates

Understand how Corning® Spheroid Microplates can be used for generating and culturing tumor spheroids in a 96-well spheroid microplate format.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Analysis of Tetrahydrocannabinol (THC) and Carboxytetrahydrocannabinol (THCCOOH) in Surface Waters by SPME and GC/MS

Glycol Ethers Properties & Examples, Organic Solvents

Glycol ethers, with both an ether and alcohol functional group in the same molecule, are one of the most versatile classes of organic solvents.

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Column Packing and Preparation for Size Exclusion Chromatography

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Self-Assembly in Organic Thin Film Transistors for Flexible Electronic Devices

Flexible electronic circuits and displays based on organic active materials are future generations of products that may eventually enter mainstream electronics market.

Few Monolayer Atomic Layer Deposition (ALD) on Surfaces and Interfaces for Energy Applications

Pressure-Temperature Calculator for Solvents

Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.