250 U, 1 U/μl, 200 reactions in a final volume of 50 μl 11 647 679 001
1,000 U, 4 x 250 U, 800 reactions in a final volume of 50 μl 11 647 687 001
Water, PCR Grade 25
thawing, prepare sufficient Growth Factor Reduced (GFR) Matrigel for 12 domes at 25 µL per dome + 5% overage (315 µL total).
Note: GFR Matrigel will gel at room temperature; maintain on ice at all times.
Activity of Typical Lot
315–426 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5 mM
glycerol 2-phosphate, 25 mM MgCl2, 5 mM EGTA, and
2 mM EDTA. Just