were used. The binding sites for these two pairs of ZFNs are
separated by 206 base pairs on the genomic DNA (see figure 1).
ZFN activity by both of these ZFN pairs on the wild
isolation; mean 99.3% ranging from 99 to 99.7%), both fromMiltenyi Biotec GmbH (cat no
130-091-104 and 130-096-535, respectively). T cells were activated with 1μg OKT-3/mL
(Biolegend, cat no 317315)