207-536-6 | Sigma-Aldrich

Quality Control Ranges: MILLIPLEX® MAP Human Cytokine/ Chemokine Magnetic Bead Panel

183 pg/mL Control 2 523 - 1086 pg/mL Control 2 465 - 966 pg/mL IL-6 Control 1 100 - 207 pg/mL

Quality Control Ranges: MILLIPLEX® MAP Human Cytokine/ Chemokine Magnetic Bead Panel

Control 1 123 – 255 pg/mL VEGF Control 1 108 – 224 pg/mL Control 2 588 – 1222 pg/mL Control 2 536 – 1112 pg/mL

2537 2910 3230 MaeIII 14 MboII 8 536 1289 2080 2151 2906 2984 3093 3289 MmeI 2 1633 1817 MnlI 19 MscI 1 389 MseI 16 MslI 6 405 600 991 2563 2722 3081 MspI 16 MspA1I 6 86 1009 1128 1760 2005 2946

TB430 pET-52b(+) Vector Map

901 1030 1153 BstYI 10 195 663 1875 2155 3604 3615 3926 3943 4711 4723 BstZ17I 1 2734 BtgI 2 268 536 BtsI 4 1464 1832 4154 4174 Enzyme # Sites Locations ClaI 1 376 DraI 3 3995 4687 4706 DraIII 1 4985

XP725 Antibody Number-Lot Number 071M4826

484 505 506 507 508 529 530 531 532 553 554 555 556 6.2 485 486 487 488 509 510 511 512 533 534 535 536 557 558 559 560 6.3 489 490 491 492 513 514 515 516 537 538 539

TB162VM pET-30 Ek/LIC Vector Map

BanI 10 251 327 515 536 650 1113 1832 1962 2088 5234 BanII 7 190 235 577 591 1404 4151 5272 BbsI 4 1339 1678 2052 2412 BbvI 25 BccI 14 Bce83I 6 21 2007 2177 3385 3683 3924 BcefI 6 712 1053

TB330VM pET-44a-c(+) Vector Map

Tag™ coding sequence 747–791 Multiple cloning sites (Sma I-Xho I) 530–724 HSV•Tag® coding sequence 536–571 His•Tag® coding sequence 512–529 T7 terminator 26–73 lacI coding sequence 2833–3915 pBR322 origin

Examination of Non-Specifically Bound Proteins and Peptides Following Depletion of Twenty High Abundance Proteins from Human Plasma

94 2 241 4 341 6 IPI00418422 IGHD protein 79 1 47 1 IPI00448938 IGHG1 410 7 89 2 463 8 349 6 513 8 736 9 IPI00472345 IGHG3 protein 83 1 163 3 207 4 212

XP725 Antibody List Lot 089K4791

P2482 207 AKT1 M NP_001014431.1,NP_001014432.1 , NP_005154.2, y y y 575 Protein Kinase Ba /Akt1 P1601 207 AKT1 P NP_001014431.1,NP_001014432.1 , NP_005154.2, y y y 577 phospho-PKB (pSer473) P4112 207 AKT1

XP725 Antibody List Lot 129K4830

P2482 207 AKT1 M NP_001014431.1,NP_001014432.1 , NP_005154.2, y y y 575 Protein Kinase Ba /Akt1 P1601 207 AKT1 P NP_001014431.1,NP_001014432.1 , NP_005154.2, y y y 577 phospho-PKB (pSer473) P4112 207 AKT1

XP725 Antibody List Lot 129K4831

P2482 207 AKT1 M NP_001014431.1,NP_001014432.1 , NP_005154.2, y y y 575 Protein Kinase Ba /Akt1 P1601 207 AKT1 P NP_001014431.1,NP_001014432.1 , NP_005154.2, y y y 577 phospho-PKB (pSer473) P4112 207 AKT1

XP725 Antibody List

001101103.1 M y y y 574 Protein Kinase Bα /Akt1 P2482 207, 11651, 24185 AKT1,Akt1 NP_005154.2,NP_033782.1,NP_150233.1 M y y y 575 Protein Kinase Bα /Akt1 P1601 207, 11651, 24185 AKT1,Akt1 NP_005154.2,NP_033782.1

Perfection in every dimension 2014. Inorganic reagents Catalogue

Measured in the ISO 17025 accredited laboratory Certipur® products Advantages 207 207 207 207 207 207 Page 206 Certipur® secondary reference materials in

XP725 Antibody List 013M4793

001101103.1 M y y y 574 Protein Kinase Bα /Akt1 P2482 207, 11651, 24185 AKT1,Akt1 NP_005154.2,NP_033782.1,NP_150233.1 M y y y 575 Protein Kinase Bα /Akt1 P1601 207, 11651, 24185 AKT1,Akt1 NP_005154.2,NP_033782.1

XP725 Worksheet for Lot Number 071M4826

F7926 532 3 4 1 4 FAK pTyr397 F7926 533 3 4 1 5 FAK pTyr577 F8926 534 3 4 1 6 FAK pTyr577 F8926 535 3 4 1 7 Falkor PHD1 F5303 536 3 4 1 8 Falkor PHD1 F5303 537 3 4 2 1

List of Antibodies

Regulator) P9489 P y n/d y 534 p38 MAP Kinase, Non-Activated M8432 M y y y 535 p38 MAPK M0800 P n/d y y 536 p38 MAPK activated (diphosphorylated p38) M8177 M y y y 537 Negative Control NA 538 p53 P5813 M y n

Halal Certificate

MALATE 97%, FG W237418 A14166 HC-24SI9963 535. DIETHYL MALONATE 98% W237507 B27853 HC-24SIYX69 536. DIETHYL SEBACATE 98%, FCC, FG W237604 B29929 HC-24SIC682 This is to certify that the following

Halal Certificate

MALATE 97%, FG W237418 A14166 HC-22SIBH61 535. DIETHYL MALONATE 98% W237507 B27853 HC-22SI5I67 536. DIETHYL SEBACATE 98%, FCC, FG W237604 B29929 HC-22SI3N80 This is to certify that the following

Acta Interactive Archive Vol 51

. 2007, 129, 7961. (13) Skowerski, K.; Czarnocki, S. J.; Knapkiewicz, P. ChemSusChem 2014, 7, 536. (14) (a) Polyzos, A.; O’Brien, M.; Petersen, T. P.; Baxendale, I. R.; Ley, S. V. Angew. Chem.