This application note describes the fast and efficient separation of the EU’s list of 15 + 1 polynuclear aromatic hydrocarbons (PAHs) using Ascentis® Express PAH HPLC column.

Milli-Q® IQ 7003/05/10/15 Ultrapure & Pure Water Purification Systems

Thank you for your interest in the Milli-Q® IQ 7003/05/10/15 Ultrapure & Pure Water Purification Systems offering. For additional information on this offering or to be contacted by a Sales Representative, please complete and submit the form below.

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

SIG10 Chemically Competent Cells

For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.

Nickel Carbonyl

Analysis of nickel carbonyl, nickel carbonyl in air, organometallics in air, exposure limits for nickel carbonyl, ni(co)4

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Milli-Q^® IQ 7003/05/10/15 Ultrapure & Pure Lab Water Purification System

Milli-Q® IQ 7003/05/10/15 lab water systems provide consistent-quality Type 1 and Type 2 water, are intuitive to use, and can support your lab's sustainability goals.

Chromolith® WidePore 300 HPLC Columns

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Software Simplifies Compliance with 21 CFR Part 11 and EudraLex Good Manufacturing Practice Volume 4 Annex 11

How software can simplify 21 CFR Part 11 and EudraLex Volume 4 Annex 11 compliance and provide additional benefits such as access control, audit trails and easier search and retrieval.

Heavy Metal Analysis of Cannabis sativa by ICP-MS and the Need for Proper Sample Homogenization

A 4-step comprehensive ICP-MS workflow including sample homogenization, with standard addition calibration method, to determine heavy metals in Cannabis sativa.

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

UHPLC Analysis of Nucleosides on Supel™ Carbon LC Column: Performance Comparisons with Competitor

Ultra-high performance liquid chromatographic (UHPLC) separation of 12 nucleosides using Supel™ Carbon LC column in 15 minutes for use in identification and quantitation of nucleoside biomarkers.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Media Sterilization – Plant Tissue Culture Protocol

Plant tissue culture media are generally sterilized by autoclaving at 121 °C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel.

Other Classic Media

A large collection of more specialized media for you cell culture applications, including L-15 Medium (Leibovitz), Fischer's Medium, Iscove's Modified Dulbecco's Medium (IMDM) and many more

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Addressing Our Environmental Impact and Value Chain

Composed of 15 categories that cover our upstream and downstream value chain activity, Scope 3 emissions are a major part of our environmental impact. Learn how we’re tackling these indirect emissions.

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

Polynuclear Aromatic Hydrocarbons Analysis by HPLC for Olive Oil

Highly accurate HPLC-FLD method for evaluating 15 PAHs in olive oil using Ascentis® Express PAH HPLC column and SPE cleanup, following GB 5009.265-2021.

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