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X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

MilliporeSigma Expands in Gillingham, UK, Boosts Distribution Capabilities

MilliporeSigma today announced the completion of a £9 million expansion to its Gillingham distribution center.

How to Boost AAV Titer with Feed Supplementation in both HEK293 and Sf-9 Platforms

In this webinar, you will learn wow to boost AAV titers in HEK293 and Sf-9 cell lines as well as getting some insider tips on volume and timing optimization.

Accelerated mAb Development Program – From Transfection to GMP Drug Substance in 9 Months

The accelerated mAb development program delivers GMP drug substance nine months from transfection. We designed the mAbExpress™ program to be fast, cost effective, and appropriate for the early clinical phase of your program, with regulatory support throughout.

Analysis of 17 Cannabinoids in Hemp and Cannabis

HPLC separation of 17 important cannabinoids including CBD, delta 9 THC and THCA. Read the application note.

Determination of Phosphorus Total in Meat and Meat Products

Colorimetric total phosphorus analysis method for meat and meat products as per German Food and Feed Code §64 LFGB 06.00-9.

Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH

Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.

Analysis of Antioxidants in Bakery Products

HPLC-UV method detects 9 antioxidants like propyl gallate in food using a Discovery®</sup HS C18 column for excellent peak shapes.

New GC-MS Method for Mycotoxin Analysis

An article regarding A New GC-MS Method for Mycotoxin Analysis Using 13C-marked Mycotoxin Derivatives from Sigma-Aldrich.com

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Mesoporous Materials: Properties and Applications

Mesoporous Materials include a range of high surface area porous silicates with applications in gas adsorption, drug delivery, diagnostics and catalysis.

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Organic Semiconductor Laser Materials

Authenticated Breast Cancer Cell Lines for Cancer Research

Choose from more than 30 breast cancer cell lines authenticated by ECACC, classified by hormone receptor-positive, HER2-positive, and triple-negative cancers, as well as by known mutations such as BRCA2.

Fluorescein Isothiocyanate-Dextran

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

Revolution in Plastic Recycling: Laser Marking & Digital Product Passports Enable True Circular Economy

An industry-wide project demonstrates how packaging can become smarter, more sustainable, and fully traceable. Plastics are a blessing—and a global problem. Lightweight, durable, and versatile. Yet these very properties make plastics a challenge worldwide. Over 400 million tons are produced

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Tips & Tricks for Thin-Layer Chromatography

Thin Layer Chromatography Troubleshooting: This article details TLC tips and tricks that one must consider while performing different steps of the technique.

Progress for High Performance Tandem Organic Solar Cells

Professor Chen (Nankai University, China) and his team explain the strategies behind their recent record-breaking organic solar cells, reaching a power conversion efficiency of 17.3%.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

Boron Nitride Nanotubes

Boron nitride nanotubes (BNNTs) are structural analogs of carbon nanotubes, with alternating boron and nitrogen atoms replacing carbon.

2D MXenes Ink: Synthesis, Properties, and Their Precursors

Optimizing the synthesis of high-quality 2D MXene flakes for 3D ink printing is essential to such technological developments as printable and flexible electronics.

Recent Developments in Silicon Anode Materials for High Performance Lithium-Ion Batteries

Recent demand for electric and hybrid vehicles, coupled with a reduction in prices, has caused lithium-ion batteries (LIBs) to become an increasingly popular form of rechargeable battery technology.

Understanding and Successfully Applying Materials for Dye-Sensitized Solar Cells

While dye sensitization as the basis for color photography has been accepted for a very long time,1 attempts to use this principle for the conversion of solar light to electricity generally had resulted only in very low photocurrents, below 100

Graphene-Based Composites and their Unique Renewable Energy Applications

Graphene is a unique two-dimensional (2D) structure of monolayer carbon atoms packed into a dense honeycomb crystal that has attracted great interest due to its diverse and fascinating properties.

Poly(ethylene glycol) (PEG) and Synthetic PEG Derivatives for Tissue Engineering and Cell Delivery

Highlighting new synthetic modifications of PEG to improve the mechanical properties and degradation of resulting hydrogels in tissue engineering applications.

Delivery of Nucleic Acids Using Polymers

Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility

Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as

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