230-27-3

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Glycogen Synthase Kinase 3 (GSK-3) Overview

Glycogen synthase kinase 3 (GSK-3) is a highly conserved family of serine/threonine kinases for over 100 proteins in many pathways. See a list of accepted GSK-3 modulators and related products.

GC Analysis of Omega 3 in Fish Oil and Salmon

Analyze omega 3 fatty acid profiles from fish oil capsules and salmon using GC columns for confirmatory analysis of lipid composition.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

Quantitative Analysis of PFAS in Drinking Water by LCMS

Simultaneous determination of 27 PFAS chemical compounds after enriching the water samples with SPE using a Supelclean® ENVI-WAX weak anion exchange tube.

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt

Spectrin

Spectrin is the major component of the protein network, which covers the cytoplasmic surface of vertebrate, erythrocyte membranes. It is a high molecular weight heterodimer, composed of two subunits (molecular weights of approximately 230 kDa and 250 kDa)

Cholesterol Biosynthesis

Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.

Cultrex ® 3-D Culture Matrix™ Laminin I Protocol

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

MilliporeSigma Partners with Promega to Advance 3-D Cell Drug Discovery Technologies

MilliporeSigma and Promega will co-develop novel technologies that advance drug discovery.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Graphene and New Monoatomic Materials: Using 2-Dimensional Nanosheets in a 3-Dimensional World

Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol

3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.

What You Need to Know About the New ISO 16140-3 Microbiological Method Verification

This on-demand webinar introduces the new EN ISO 16140-3 on the verification of methods in food microbiology.

Cell Bio Poster Tube Contest

Are you presenting a poster at Cell Bio December 14-18th, 2024 in San Diego? Carry your poster in style with a MilliporeSigma Poster Tube Carrier. Complete the form below by November 27, 2024 for your chance to win. Contest for

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

GPCR Markers for Cancer Research

Explore the role of some selected GPCR markers in cancer research development and treatment, including neurohormonal GPCRs, rhodopsin-like GPCRs, and more.

Determination of Water Content in Ethyl acetate, acetic acid ethyl ester Using Karl Fischer Titration

Accurately measure moisture content in ethyl acetate (CH3CO2CH2CH3) through Karl Fischer titration, using both volumetric and coulometric methods for reliable results.

Custom siRNA Design Service Request Form

Please fill out the form to receive a complimentary siRNA design. Requests are typically completed within 3 working days.

Viral Safety in Bioprocessing

Viral safety in bioprocessing relies on a framework of (1) preventing contamination, (2) detecting contamination, and (3) removing or inactivating viral contaminants.

Supplier Sustainability Toolkits

Empower your organization with practical resources and best practices to implement sustainable practices, lowering Scope 1, 2, and 3 emissions.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Sustainable Supply Chains

Global legal guidelines are increasingly forcing transparency in global and local supply chains. But when we demand more information, data and security from our own scope-3 suppliers, we should realize we ourselves are our customers' scope-3 suppliers. We're all in

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Neuronal Mesenchymal Stem Cells

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Hydrosilylation Catalyst

Trost group's protocol yields α-vinylsilanes from terminal acetylenes using [Cp*Ru(MeCN)3]PF6 catalyst and others for hydrosilylation.

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