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232-462-6
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Showing 1-30 of 736 results for "232-462-6" within Site Content
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
Ni Sepharose 6 Fast Flow for Protein Purification
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Oligonucleotide Standard 6 Mix LC-UV Analysis
Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility
Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as
Dextran
Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.
Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV
Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.
USDA FSIS STEC Guidance Implementation
Discover the expanded USDA FSIS verification testing for the 'Big 6' non-O157 STEC in beef products and explore accurate testing solutions and industry practices for enhanced food safety.
Determination of Water Content in Phenol Using Karl Fischer Titration
Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Preservation of Moisture-Sensitive Chemical Reagents
Preserve reagent quality of air- and moisture-sensitive reagents using nitrogen or argon in crown-cap bottles with a 6 mm diameter hole in the crown-cap and a PTFE-faced rubber liner.
Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin
This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.
Mesoporous Materials: Properties and Applications
Mesoporous Materials include a range of high surface area porous silicates with applications in gas adsorption, drug delivery, diagnostics and catalysis.
Purification of NAD+ and ATP-dependent Kinases
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
Titration with the Titrette® Bottletop Burette
This article decribes how to use the BRAND® Titrette® Digital Bottletop Burette for quick and reliable titration.
Detection of Genomic DNA from Processed Plant Oils and Wheat Flour
Food processing practices are common in today's agricultural and food industries, and are often harsh on the genetic information of plants and plant products.
Immunohistochemistry: Current Applications in Breast Cancer
Immunohistochemistry (IHC) is used to characterize intracellular proteins or various cell surfaces in all tissues. Individual markers or more often panels of various marker proteins can be used to characterize various tumor subtypes, confirm tissue of origin, distinguish metastatic from
Serum free cancer cell line media
Defined culture conditions. Consistent growth of human cancer cell lines in PromoCell’s serum-free/xeno-free Cancer Cell Line Media-XF. Explore over 350 PromoCell products on Sigma.com
Formamide Molecular Biology Reagent
Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.
Formulation and Delivery US 2024
Join us at the Formulation and Delivery US conference at booth #6 to learn about our integrated offering for all process steps in pharmaceutical and biopharmaceutical manufacturing which includes products that meet the highest quality and purity standards with extensive
Thermal Transitions of Homopolymers: Glass Transition & Melting Point
Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.
Organic Semiconductor Laser Materials
Organic Semiconductor Laser Materials
2-Hydroxyglutarates Analysis by LCMS
Reversed phase application for the analysis of S-2-hydroxyglutaric acids and R-2-hydroxyglutaric acids by chiral LCMS, using a CHIROBIOTIC® R Column.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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