Chemistry (Weinheim an der Bergstrasse, Germany), 18(41), 12986-12998 (2012-09-13)
Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the...
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of...
STED with wavelengths closer to the emission maximum.
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence...
Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplement.
Tian, Q.; et al.
Journal of Molecular and Cellular Cardiology, 52(1), 113-124 (2012)
Ultrafast photon counting applied to resonant scanning STED microscopy.
Wu, X.; Toro, L.; Stefani, E.; Wu, Y.
Journal of Microscopy, 257(1), 31-38 (2015)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
Resolving the structure of inner ear ribbon synapses with STED microscopy.
Synapse, 69(5), 242-255 (2015)
Analysis of replication factories in human cells by super-resolution light microscopy.
Cseresnyes, Z.; et al.
BMC Cell Biology, 10(1), 88-88 (2009)
Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye.
Luo, D., et al.
Optics & Laser technology, 45, 723-725 (2013)
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
Vicidomini, G.; et al.
Nano Letters, 15(9), 5912-5918 (2015)
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices.
A new approach for the determination of the amyloid fibril - thioflavin T (ThT) binding parameters (the number of binding modes, stoichiometry, and binding constants of each mode) is proposed. This approach is based on the absorption spectroscopy determination of...
STED nanoscopy. A glimpse into the future.
Bianchini, P.; et al.
Cell and Tissue Research, 360(1), 143-150 (2015)
Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95.
Nair D.; et al.
The Journal of Neuroscience, 33(32), 13204-13224 (2013)
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100...
Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells.
Stagge, F.; et al.
PLoS ONE, 8(10), e78745-e78745 (2013)
Quantification of Internalized Silica Nanoparticles via STED Microscopy.
Peuschel, H.; et al.
BioMed Research International, 2015(1), 1-16 (2015)
Far-field optical nanoscopy based on continuous wave laser stimulated emission depletion.
Kuang, C.; Zhao, W.; Wang, G.; et al.
The Review of Scientific Instruments, 81(5), 53709-53709 (2010)
STED Microscopy to Monitor Agglomeration of Silica Particles Inside A549 Cells.
Schubbe, S.; Cavelius, C.
Advanced Energy Materials, 12, 417-422 (2010)
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope.
Meyer, S. A.; Ozbay, B.
Proceedings of SPIE, 8950, 89500-89500 (2014)
Munc18-1 Tuning of Vesicle Merger and Fusion Pore Properties.
Jorgacevski, J.; et al.
The Journal of Neuroscience, 31(24), 9055-9066 (2011)
Exploring single-molecule dynamics with fluorescence nanoscopy.
Ringemann, Ch.; et al
New Journal of Physics, 11(10), 103054-103054 (2009)
Enhancing stimulated emission-based fluorescence detection with interferometric setup.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(4), 481-491 (1997-04-01)
Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are...
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