Showing 1-30 of 45 results for "286958"
H Peck et al.
Clinica chimica acta; international journal of clinical chemistry, 94(3), 237-240 (1979-06-15)
The urine of untreated phenylketonurics in the first weeks of life contains gamma-glutamylphenylalanine (0.07--0.69 mmol/g creatinine, 12 samples) visible on the normal 2-dimensional electrophoreto-chromatogram. This compound is less prominent or absent when urine from older untreated phenylketonurics is examined and...
Sumit Chakraborty et al.
Biochemistry, 47(12), 3800-3809 (2008-03-05)
Direct spectroscopic observation of thiamin diphosphate-bound intermediates was achieved on the enzyme benzaldehyde lyase, which carries out reversible and highly enantiospecific conversion of ( R)-benzoin to benzaldehyde. The key enamine intermediate could be observed at lambda max 393 nm in...
Faqing Tang et al.
Clinical biochemistry, 44(8-9), 711-718 (2011-03-16)
To search for markers of nasopharyngeal carcinoma (NPC) for diagnosis. Using gas chromatography and mass spectrometry, we evaluated 51 serum metabolites in 49 NPC, 37 throat cancer patients and 40 healthy controls. High metabolites were selected and confirmed in NPC...
Jianghua Jia et al.
Applied biochemistry and biotechnology, 162(1), 242-251 (2009-09-24)
Two DNA fragments containing the entire coding sequences of lactate dehydrogenase (LDH; ldhL1 and ldhD), whose enzymes have high activity for bioconversion of phenylpyruvate (PPA) to phenyllactate (PLA), were amplified from Lactobacillus plantarum SK002 using PCR. Sequencing showed open reading...
Tapan Kanti Paine et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 13(21), 6073-6081 (2007-05-01)
Iron(II)-phenylpyruvate complexes of tetradentate tris(6-methyl-2-pyridylmethyl)amine (6-Me3-TPA) and tridentate benzyl bis(2-quinolinylmethyl)amine (Bn-BQA) were prepared to gain insight into C-C bond cleavage catalyzed by dioxygenase enzymes. The complexes we have prepared and characterized are [Fe(6-Me3-tpa)(prv)][BPh4] (1), [Fe2(6-Me3-tpa)2(pp)][(BPh4)2] (2), and [Fe2(6-Me3-tpa)2(2'-NO2-pp)][(BPh4)2] (3), [Fe(6-Me3-tpa)(pp-Me)][BPh4]...
Satoshi Hirano et al.
The Journal of biological chemistry, 283(10), 6459-6466 (2008-01-04)
Violacein and the indolocarbazoles are naturally occurring bisindole products with various biological activities, including antitumor activity. Although these compounds have markedly different molecular skeletons, their biosynthetic pathways share the same intermediate "compound X," which is produced from L-tryptophan via indole-3-pyruvic...
J M Turchany et al.
Journal of autoimmunity, 6(4), 459-466 (1993-08-01)
The identification and cloning of the mitochondrial autoantigens in primary biliary cirrhosis (PBC) have provided new clues in disease pathogenesis. The two major autoantigens are the E2 subunits of pyruvate dehydrogenase and branched-chain ketoacid dehydrogenase (BCKD). Interestingly, one of these...
Dongli Zhang et al.
Analytica chimica acta, 694(1-2), 61-66 (2011-05-14)
The urinary metabolic marker compounds, namely phenylpyruvic acid (PPA), 2-hydroxyphenylacetic acid (oOPAA), 4-hydroxyphenylacetic acid (pOPAA), phenyllactic acid (PLA) and phenylacetic acid (PAA) of phenylketonuric individuals were detected by a novel method of micellar electrokinetic capillary chromatography with capacitively coupled contactless...
Hajer Ouertatani-Sakouhi et al.
Journal of biomolecular screening, 15(4), 347-358 (2010-03-17)
Macrophage migration inhibitory factor (MIF) is a major mediator of innate immunity and inflammation and presents a potential therapeutic target for various inflammatory, infectious, and autoimmune diseases, including cancer. Although a number of inhibitors have been identified and designed based...
Nucleophile selectivity of chorismate-utilizing enzymes.
Olivier Kerbarh et al.
Chembiochem : a European journal of chemical biology, 8(6), 622-624 (2007-03-06)
Andrea Pereira Rosa et al.
Cellular and molecular neurobiology, 32(7), 1113-1118 (2012-04-06)
Phenylketonuria is a recessive autosomal disorder that is caused by a deficiency in the activity of phenylalanine-4-hydroxylase, which converts phenylalanine to tyrosine, leading to the accumulation of phenylalanine and its metabolites phenyllactic acid, phenylacetic acid, and phenylpyruvic acid in the...
L Cassidei et al.
Clinica chimica acta; international journal of clinical chemistry, 90(2), 121-127 (1978-12-01)
A simple alternative colorimetric method has been developed for quantitative determination of phenylpyruvic acid in urine based on the improved stability of the ferric complex of enol-phenylpyruvate in water-dimethylsulfoxide 50% vol. mixed solvent. The method can be applied directly to...
A Hargreaves et al.
Journal of photochemistry and photobiology. B, Biology, 89(2-3), 110-116 (2007-11-06)
Ultraviolet A (UVA) light (315-400 nm) is ubiquitously found in our environment and constitutes about 95% of the total solar UV; all UVC and most UVB being absorbed by the stratospheric ozone layer. Compared with UVB and C, UVA does...
Taiki Fujii et al.
Biochimica et biophysica acta, 1814(12), 1669-1676 (2011-06-16)
We discovered the phenyllactate (PLA)-producing fungal strain Wickerhamia fluorescens TK1 and purified phenylpyruvate reductase (PPR) from fungal cell-free extracts. The PPR used both NADPH and NADH as cofactors with more preference for the former. The enzyme reaction as well as...
Wanmeng Mu et al.
Bioresource technology, 100(21), 5226-5229 (2009-06-09)
3-Phenyllactic acid (PLA), which is produced by some strains of lactic acid bacteria (LAB), is a known antimicrobial agent with a broad spectrum. Batch and fed-batch fermentation by the strain Lactobacillus sp. SK007 for PLA production have been reported. With...
Cristiana M L Di Giuro et al.
Journal of inorganic biochemistry, 105(9), 1204-1211 (2011-07-02)
Mononuclear nonheme iron enzymes (MNHEs) catalyze a range of very diverse reactions in O(2) metabolism, but they share a common principle active-site organization. To investigate a putative catalytic promiscuity of these enzymatic metal centers, we studied the reactivity of the...
T L Perry et al.
Clinica chimica acta; international journal of clinical chemistry, 117(1), 7-12 (1981-11-25)
Cystinylglycine, recently identified as a normal small peptide in human plasma, has diagnostic importance for several genetically determined disorders. We found cystinylglycine absent from the plasma of a patient with pyroglutamic acidemia, and the peptide was either absent or greatly...
A M Paloian et al.
Prikladnaia biokhimiia i mikrobiologiia, 49(2), 129-135 (2013-06-26)
Km for L-phenylalanine, L-glutamic acid, L-aspartic acid, and the corresponding keto acids were calculated, as well as Vmax, was measured for the following pairs of substrates: L-phenylalanine-2-ketoglutarate, L-phenylalanine-oxaloacetate, L-glutamic acid-phenylpyruvate, and L-aspartic acid-phenylpyruvate for aminotransferases PATI, PAT2, and PAT3 from...
Wanmeng Mu et al.
Biotechnology letters, 34(5), 907-911 (2012-01-21)
The gene coding for D-lactate dehydrogenase (D-LDH) from Pediococcus acidilactici DSM 20284 was cloned and expressed in E. coli. The recombinant enzyme was purified by nickel-affinity chromatography. It converted phenylpyruvic acid (PPA) to 3-phenyllactic acid maximally at 30°C and pH...
K Nikkilä et al.
Hepatology (Baltimore, Md.), 13(4), 663-669 (1991-04-01)
Serum levels of cholesterol precursors (squalene, delta 8-cholestanol, desmosterol and lathosterol), plant sterols (campesterol and sitosterol), cholestanol and cholestanol/noncholesterol sterol ratios were related to liver damage and liver transplantation indications in healthy controls (n = 26) and in 31 patients...
Shuhuai Yu et al.
Biotechnology letters, 36(3), 627-631 (2013-11-20)
3-Phenyllactic acid (PLA) is an antimicrobial compound with broad and effective antimicrobial activity against both bacteria and fungi. Enzymatic production of PLA can be carried out from phenylpyruvic acid by lactate dehydrogenase (LDH); however, the enzymatic reaction is accompanied by...
K Michals et al.
The American journal of clinical nutrition, 42(2), 361-365 (1985-08-01)
The metabolites of phenylalanine, phenylacetate, phenyllactate, phenylpyruvate and phenylethylamine, were measured in the urine of PKU patients. In general correlation was found between serum phenylalanine excretion of these metabolites. However, there were individual variations in the quantities and type of...
J M Lasala et al.
Science (New York, N.Y.), 203(4377), 283-284 (1979-01-19)
3',4'-Deoxynorlaudanosolinecarboxylic acid (DNLCA), a tetrahydroisoquinoline derived from dopamine and phenylpyruvic acid, has been detected by computerized mass fragmentography in urine of phenylketonuric children and in urine and brain of rats with experimentally induced hyperphenylalaninemia. Levels of DNLCA in brain of...
Tânia Veiga et al.
Eukaryotic cell, 11(2), 238-249 (2011-12-14)
The industrial production of penicillin G by Penicillium chrysogenum requires the supplementation of the growth medium with the side chain precursor phenylacetate. The growth of P. chrysogenum with phenylalanine as the sole nitrogen source resulted in the extracellular production of...
Zhaojuan Zheng et al.
PloS one, 6(4), e19030-e19030 (2011-05-03)
Phenyllactic acid (PLA), a novel antimicrobial compound with broad and effective antimicrobial activity against both bacteria and fungi, can be produced by many microorganisms, especially lactic acid bacteria. However, the concentration and productivity of PLA have been low in previous...
Shuhuai Yu et al.
Bioscience, biotechnology, and biochemistry, 76(4), 853-855 (2012-04-10)
D-Lactate dehydrogenase (D-LDH) from Pediococcus pentosaceus ATCC 25745 was found to produce D-3-phenyllactic acid from phenylpyruvate. The optimum pH and temperature for enzyme activity were pH 5.5 and 45 °C. The Michaelis-Menten constant (K(m)), turnover number (k(cat)), and catalytic efficiency...
Paddy Jim Baggot et al.
Fetal diagnosis and therapy, 23(3), 245-248 (2008-04-18)
Organic acids were examined from normal and Down syndrome pregnancies to identify possible differences between the amniotic fluid from fetuses with Down Syndrome compared with that of normal fetuses. Amniotic fluids were obtained from prior amniocenteses. Forty-one normal and 22...
Olivier Boulat et al.
Clinical chemistry and laboratory medicine, 41(12), 1642-1658 (2004-01-08)
Organic acid analysis is used for the early detection/ exclusion and for the follow-up of inherited disorders of amino acid and organic acid metabolism. Urinary organic acid concentrations in 417 healthy Caucasian children (1 day to 17 years of age)...
Xingfeng Li et al.
Biotechnology letters, 29(4), 593-597 (2007-03-03)
Phenyllactic acid (PLA) is a novel antimicrobial compound derived from phenylalanine (Phe). Lactobacillus sp. SK007, having high PLA-producing ability, was isolated from Chinese traditional pickles. When 6.1 mM phenylpyruvic acid (PPA) was used to replace Phe as substrate at the...
Qiang Tan et al.
Applied biochemistry and biotechnology, 136(3), 279-289 (2007-07-13)
The high-density fermentation of recombinant Pichia pastoris was carried out in a 1-L fermentor. After 60 h of fermentation, the activities of D-amino acid oxidase (DAAO) and catalase assayed with the permeabilized cells attained 12,532 and 684,800 U/L, respectively. Additionally...

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