Showing 1-30 of 37 results for "39235"
J L Mpagi et al.
Molecular and biochemical parasitology, 105(2), 273-279 (2000-02-29)
The mechanism by which filarial parasites derive fatty acids bound to the host's carrier protein is poorly understood. The capacity of a secretory protein of Onchocerca volvulus (OvS1/Ov20) to compete with serum albumin for arachidonic and other fatty acids was...
N Saghir et al.
International journal for parasitology, 31(7), 653-660 (2001-05-05)
The cestode Hymenolepis diminuta contains an abundant, cytoplasmic, hydrophobic ligand, binding protein (H-HLBP). Studies with polarity sensitive probes suggest a single hydrophobic binding site, the results also indicate that the single tryptophan in the molecule (Trp41) is involved in ligand...
O K Gasymov et al.
Biochimica et biophysica acta, 1433(1-2), 307-320 (1999-10-09)
The principal lipid binding protein in tears, tear lipocalin (TL), binds acid and the fluorescent fatty acid analogs, DAUDA and 16-AP at one site TL compete for this binding site. A fluorescent competitive binding assay revealed that apo-TL has a...
A E Thumser et al.
The Biochemical journal, 320 ( Pt 3), 729-733 (1996-12-15)
The physiological role of liver fatty acid-binding protein (L-FABP) has yet to be clarified. An important feature of this member of the family of intracellular lipid-binding proteins is the wide range of compounds that have been identified as potential physiological...
K Kim et al.
Protein science : a publication of the Protein Society, 7(8), 1821-1828 (1999-03-19)
We have systematically mutated residues located in turns between beta-strands of the intestinal fatty acid binding protein (IFABP), and a glycine in a half turn, to valine and have examined the stability, refolding rate constants and ligand dissociation constants for...
M Yamaguchi et al.
The Analyst, 117(12), 1859-1861 (1992-12-01)
A highly sensitive, simple and reproducible method for the quantitative determination of fatty acid binding protein in rat liver by post-column high-performance liquid chromatography with fluorescence detection is presented. Fatty acid binding protein in rat liver cytosols is separated by...
Krzysztof Gwozdzinski et al.
Experimental physiology, 102(2), 190-201 (2016-11-20)
What is the central question of this study? What is the influence of a single bout of exercise on the properties of erythrocyte fractions at different ages? What is the main finding and its importance? A single bout of exercise...
M Rahman et al.
Parasitology, 139(10), 1361-1374 (2012-06-05)
Taenia solium, a causative agent of taeniasis and cysticercosis, has evolved a repertoire of lipid uptake mechanisms. Proteome analysis of T. solium excretory-secretory products (TsESP) identified 10 kDa proteins displaying significant sequence identity with cestode hydrophobic-ligand-binding-proteins (HLBPs). Two distinct 362-...
A E Thumser et al.
The Biochemical journal, 314 ( Pt 3), 943-949 (1996-03-15)
Rat liver fatty acid-binding protein (FABP) is able to accommodate a wide range of non-polar anions in addition to long-chain fatty acids. This property means that the liver protein is functionally different from other FABPs from intestine, muscle and adipose...
Valeria Silva-Álvarez et al.
PLoS neglected tropical diseases, 9(3), e0003552-e0003552 (2015-03-15)
The hydatid disease parasite Echinococcus granulosus has a restricted lipid metabolism, and needs to harvest essential lipids from the host. Antigen B (EgAgB), an abundant lipoprotein of the larval stage (hydatid cyst), is thought to be important in lipid storage...
Fen Qiao et al.
PloS one, 11(8), e0160003-e0160003 (2016-08-02)
Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- and retinol-binding (FAR) proteins are nematode-specific lipid...
Fabien Démares et al.
Insects, 9(4) (2018-11-06)
Potassium channels constitute a very diverse group involved in neural signaling, neuronal activity, membrane potential maintenance, and action potential generation. Here, we tested the mammalian potassium channel blockers TRAM-34 and 5-hydroxydecanoate (5-HDC), as well as certain fatty acids (FA) that...
Waseem Al-Jameel et al.
Oncotarget, 8(19), 31041-31056 (2017-04-19)
Castration resistant-prostate cancer is largely impervious to feather hormonal therapy and hence the outlook for patients is grim. Here we use an approach to attach the recently discovered Achilles heel. The experimental treatment established in this study is based on...
Anna Timanova-Atanasova et al.
Biochimica et biophysica acta, 1674(2), 200-204 (2004-09-18)
A 13-kDa fatty acid binding protein (FABP) (Fh13) has been isolated from the cytosol of adult Fasciola hepatica and its physicochemical and binding characteristics determined. Fh13 appears to exist as a dimer in native solution. Binding of the fluorescent fatty...
J K Davies et al.
Biochemistry, 38(51), 16932-16940 (1999-12-22)
A number of intracellular proteins bind to negatively charged phospholipid membranes, and this interfacial binding results in a conformational change that modulates the activity of the protein. Using a fluorescent fatty acid analogue, 11-[5-(dimethylamino)naphthalenesulfonyl]undecanoic acid (DAUDA), it is possible to...
Liver fatty acid binding protein (FABP) binds to anionic phospholipid vesicles with release of ligand.
J K Davies et al.
Biochemical Society transactions, 26(3), S238-S238 (1998-10-10)
Lionel Tcatchoff et al.
FEBS letters, 580(8), 2102-2108 (2006-03-21)
Odorant-binding proteins (OBPs) are small abundant soluble proteins belonging to the lipocalin superfamily, which are thought to carry hydrophobic odorants through aqueous mucus towards olfactory receptors. Human variant hOBP-2A has been demonstrated to bind numerous odorants of different chemical classes...
Lisi Huang et al.
Molecular and cellular biochemistry, 363(1-2), 367-376 (2011-12-23)
Clonorchis sinensis fatty acid-binding protein (CsFABP) belongs to a multigene family of lipid-binding proteins and is considered to be a promising vaccine candidate for human clonorchiasis. In this study, binding characteristics of CsFABP have been examined for the first time....
Lindsay C McDermott et al.
Biochemistry, 45(7), 2035-2041 (2006-02-16)
Zn-alpha(2)-glycoprotein (ZAG) is a soluble lipid-mobilizing factor associated with cancer cachexia and is a novel adipokine. Its X-ray crystal structure reveals a poly(ethylene glycol) molecule, presumably substituting for a higher affinity natural ligand, occupying an apolar groove between its alpha(1)...
A Timanova et al.
European journal of biochemistry, 261(2), 569-576 (1999-04-24)
A fatty acid-binding protein from the nematode Ascaridia galli was characterized. The gene was isolated and recombinantly expressed in Escherichia coli. According to the deduced amino acid sequence A. galli fatty acid-binding protein (AgFABP) belongs to the family of nematode...
Silvia L Delker et al.
Journal of structural biology, 148(2), 205-213 (2004-10-13)
Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. The previously reported 2.8 A crystal structure of ZAG isolated from human serum demonstrated the structural similarity between ZAG and class I major histocompatibility complex...
R G Maatman et al.
Biochimica et biophysica acta, 1214(1), 1-10 (1994-08-25)
Human liver fatty acid-binding protein (L-FABP) has been efficiently expressed in Escherichia coli. The cDNA encoding human liver FABP was under the control of T7 RNA polymerase promoter in the expression vector pET-3b. Expression required overnight induction with isopropyl beta-D-thiogalactopyranoside...
M Lechner et al.
The Biochemical journal, 356(Pt 1), 129-135 (2001-05-05)
Human tear lipocalin [lipocalin 1 (lcn-1); von Ebner's gland protein] is a member of the lipocalin superfamily that is known to bind an unusual variety of lipophilic ligands. Because of its properties and its tissue-specific expression it has been suggested...
Yu Wang et al.
Protein science : a publication of the Protein Society, 20(12), 2095-2101 (2011-10-15)
11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of...
A E Thumser et al.
The Biochemical journal, 307 ( Pt 1), 305-311 (1995-04-01)
Rat liver fatty acid-binding protein (FABP) is able to bind a wide range of non-polar anionic ligands, including lysophospholipids. In order to understand the nature of lysophospholipid interactions with liver FABP, the binding of natural lysophospholipids and two fluorescent analogues...
May Poh Lai et al.
Protein science : a publication of the Protein Society, 29(7), 1606-1617 (2020-04-17)
Two different members of the fatty acid-binding protein (FABP) family are found in enterocyte cells of the gastrointestinal system, namely liver-type and intestinal fatty acid-binding proteins (LFABP and IFABP, also called FABP1 and FABP2, respectively). Striking phenotypic differences have been...
Daniel A Breustedt et al.
Biochimica et biophysica acta, 1764(2), 161-173 (2006-02-08)
At least ten different lipocalins occur in the human body: retinol-binding protein (RBP), alpha1-acid glycoprotein, alpha1-microglobulin, apolipoprotein D, beta-trace protein, complement component 8gamma, glycodelin, neutrophil gelatinase-associated lipocalin, odorant-binding protein, and tear lipocalin. Although many of these lipocalins seem to play...
M W Kennedy et al.
The Journal of biological chemistry, 272(47), 29442-29448 (1997-12-31)
Ov20 is a major antigen of the parasitic nematode Onchocerca volvulus, the causative agent of river blindness in humans, and the protein is secreted into the tissue occupied by the parasite. DNA encoding Ov20 was isolated, and the protein was...
D Janssen et al.
The Biochemical journal, 311 ( Pt 1), 49-57 (1995-10-01)
A lipid-binding protein (LBP) has been purified from the cytosol of the cestode Moniezia expansa. The native LBP was found to be an oligomer of approx. 250 kDa, consisting of 11 kDa monomers. The LBP bound saturated and unsaturated fatty...
Matthew W Elmes et al.
Scientific reports, 9(1), 7588-7588 (2019-05-22)
The increasing use of medical marijuana highlights the importance of developing a better understanding of cannabinoid metabolism. Phytocannabinoids, including ∆9-tetrahydrocannabinol (THC), are metabolized and inactivated by cytochrome P450 enzymes primarily within the liver. The lipophilic nature of cannabinoids necessitates mechanism(s)...

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