Showing 1-30 of 56 results for "41051"
Jeanne C Stachowiak et al.
Nature cell biology, 14(9), 944-949 (2012-08-21)
Curved membranes are an essential feature of dynamic cellular structures, including endocytic pits, filopodia protrusions and most organelles. It has been proposed that specialized proteins induce curvature by binding to membranes through two primary mechanisms: membrane scaffolding by curved proteins...
Peter P T Surawski et al.
Molecular bioSystems, 4(7), 774-778 (2008-06-20)
The concept of optically encoding particles for solid phase organic synthesis has existed in the literature for several years. However, there remains a significant challenge to producing particles that are capable of withstanding harsh solvents and reagents whilst maintaining the...
Hans Blom et al.
International journal of molecular sciences, 11(2), 386-406 (2010-04-14)
Electrostatic interactions between dielectric surfaces and different fluorophores used in ultrasensitive fluorescence microscopy are investigated using objective-based Total Internal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacial dynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein, zwitterionic rhodamine 110 and...
E Pourkarimi et al.
Cell death and differentiation, 19(3), 406-415 (2011-09-03)
In C. elegans, the BH3-only domain protein EGL-1, the Apaf-1 homolog CED-4 and the CED-3 caspase are required for apoptosis induction, whereas the Bcl-2 homolog CED-9 prevents apoptosis. Mammalian B-cell lymphoma 2 (Bcl-2) inhibits apoptosis by preventing the release of...
Thomas Heitkamp et al.
Biochemistry, 47(11), 3564-3575 (2008-02-27)
The membrane-embedded K (+)-translocating KdpFABC complex from Escherichia coli belongs to the superfamily of P-type ATPases, which share common structural features as well as a well-studied catalytic mechanism. However, little is known about the oligomeric state of this class of...
Markus Hirsch et al.
Biological chemistry, 393(1-2), 23-35 (2012-05-26)
Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted...
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy.
Vogelsang, J.; et al.
Proceedings of the National Academy of Sciences of the USA, 106(20), 8107-8112 (2009)
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde, S.; et al.
Photochemistry and Photobiology, 10(4), 499-506 (2011)
Claudio G Rolli et al.
PloS one, 5(1), e8726-e8726 (2010-01-22)
Cell migration is a fundamental feature of the interaction of cells with their surrounding. The cell's stiffness and ability to deform itself are two major characteristics that rule migration behavior especially in three-dimensional tissue. We simulate this situation making use...
Jean A Smith et al.
The Journal of cell biology, 219(4) (2020-04-25)
Fragile-X mental retardation autosomal homologue-1 (FXR1) is a muscle-enriched RNA-binding protein. FXR1 depletion is perinatally lethal in mice, Xenopus, and zebrafish; however, the mechanisms driving these phenotypes remain unclear. The FXR1 gene undergoes alternative splicing, producing multiple protein isoforms and...
Verbesserte hochauflosende Mikroskopie mit Oxazinfarbstoffen in schwerem Wasser.
Lee, S. F.; Verolet, Q.; Furstenberg, A.
Angewandte Chemie (International Edition in English), 124(34), 9117-9120 (2013)
Anna Kułakowska et al.
Journal of fluorescence, 20(2), 563-569 (2009-12-30)
In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching...
Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes.
Hennig, S.; et al.
Nano Letters, 15(2), 1374-1381 (2015)
The changing point-spread function. Single-molecule-based super-resolution imaging.
Horrocks, M. H.; Palayret, M.; Klenerman, D.; Lee, S. F.
Histochemistry and Cell Biology, 141(6), 577-585 (2014)
Darby Kozak et al.
Langmuir : the ACS journal of surfaces and colloids, 24(4), 1204-1211 (2007-12-11)
This study presents the use of flow cytometry as a high-throughput quantifiable technique to study multicomponent adsorption interactions between proteins and surfaces. Flow cytometry offers the advantage of high-throughput analysis of multiple parameters on a very small sampling scale. This...
Intrinsically Resolution Enhancing Probes for Confocal Microscopy.
Vogelsang, J.; et al.
Nano Letters, 10(2), 672-679 (2010)
Hossein Tezval et al.
The Prostate, 69(4), 443-448 (2008-12-06)
Expression of urocortin (Ucn) in the human benign prostate and prostate cancer has been reported recently. Ucn binds and activates corticotropin releasing factor (CRF) receptor 1 (CRFR1) and 2 (CRFR2). Activation of CRFR2 has been shown to inhibit tumor growth...
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo, N. H.; et al.
The Journal of Cell Biology, 205(4), 591-606 (2014)
Dynamic Submicroscopic Signaling Zones Revealed by Pair Correlation Tracking and Localization Microscopy.
You, Ch.; et al.
Analytical Chemistry, 86(17), 8593-8602 (2014)
Beatrice Dietz et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(31), 10699-10712 (2012-08-03)
In the developing nervous system, spontaneous neuronal activity arises independently of experience or any environmental input. This activity may play a major role in axonal pathfinding, refinement of topographic maps, dendritic morphogenesis, and the segregation of axonal terminal arbors. In...
Ying Shen et al.
Nature communications, 10(1), 433-433 (2019-01-27)
Aberrant sperm flagella impair sperm motility and cause male infertility, yet the genes which have been identified in multiple morphological abnormalities of the flagella (MMAF) can only explain the pathogenic mechanisms of MMAF in a small number of cases. Here...
Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap.
Rendler, T., et al.
arXiv, 1102-1102 (2011)
Christian M Roth et al.
Analytical chemistry, 79(19), 7340-7345 (2007-09-07)
Current efforts to monitor the diffusion of proteins in living cells are based on either fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching, or image correlation spectroscopy. However, these methods cannot generate a map of diffusion times. Here, we introduce...
Fredrik Persson et al.
Nano letters, 12(5), 2260-2265 (2012-03-22)
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective...
Jonas K Hannestad et al.
ACS nano, 7(1), 308-315 (2012-12-12)
We use single-molecule fluorescence microscopy to monitor individual hybridization reactions between membrane-anchored DNA strands, occurring in nanofluidic lipid monolayer films deposited on Teflon AF substrates. The DNA molecules are labeled with different fluorescent dyes, which make it possible to simultaneously...
Silviya Zustiak et al.
Journal of biomedical optics, 17(12), 125004-125004 (2012-12-05)
Fluorescence correlation spectroscopy (FCS) is increasingly being used to assess the movement of particles diffusing in complex, optically dense surroundings, in which case measurement conditions may complicate data interpretation. It is considered how a single-photon FCS measurement can be affected...
Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Muller, P., et al. et al.
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
Ragunathan Priya et al.
Journal of bioenergetics and biomembranes, 40(4), 245-255 (2008-08-01)
The first low resolution solution structure of the soluble domain of subunit b (b (22-156)) of the Escherichia coli F(1)F(O) ATPsynthase was determined from small-angle X-ray scattering data. The dimeric protein has a boomerang-like shape with a total length of...
Dynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-High Density.
Giannone, G.; et al.
Biophysical Journal, 99(4), 1303-1310 (2010)
Anne Månsson et al.
Journal of leukocyte biology, 85(4), 719-727 (2009-01-09)
Viral respiratory infections are increasingly implicated in allergic exacerbations. The mechanisms behind this are not known, but a virus-induced activation of eosinophils through TLRs could be involved. Herein, we investigated the expression and function of TLR7 and TLR9 in purified...

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