Showing 1-30 of 62 results for "41698"
Jeanne C Stachowiak et al.
Nature cell biology, 14(9), 944-949 (2012-08-21)
Curved membranes are an essential feature of dynamic cellular structures, including endocytic pits, filopodia protrusions and most organelles. It has been proposed that specialized proteins induce curvature by binding to membranes through two primary mechanisms: membrane scaffolding by curved proteins...
Peter P T Surawski et al.
Molecular bioSystems, 4(7), 774-778 (2008-06-20)
The concept of optically encoding particles for solid phase organic synthesis has existed in the literature for several years. However, there remains a significant challenge to producing particles that are capable of withstanding harsh solvents and reagents whilst maintaining the...
The changing point-spread function. Single-molecule-based super-resolution imaging.
Horrocks, M. H.; Palayret, M.; Klenerman, D.; Lee, S. F.
Histochemistry and Cell Biology, 141(6), 577-585 (2014)
Darby Kozak et al.
Langmuir : the ACS journal of surfaces and colloids, 24(4), 1204-1211 (2007-12-11)
This study presents the use of flow cytometry as a high-throughput quantifiable technique to study multicomponent adsorption interactions between proteins and surfaces. Flow cytometry offers the advantage of high-throughput analysis of multiple parameters on a very small sampling scale. This...
Qian Peter Su et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15036-15046 (2020-06-17)
Mammalian DNA replication is initiated at numerous replication origins, which are clustered into thousands of replication domains (RDs) across the genome. However, it remains unclear whether the replication origins within each RD are activated stochastically or preferentially near certain chromatin...
Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Muller, P., et al. et al.
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
Thomas Heitkamp et al.
Biochemistry, 47(11), 3564-3575 (2008-02-27)
The membrane-embedded K (+)-translocating KdpFABC complex from Escherichia coli belongs to the superfamily of P-type ATPases, which share common structural features as well as a well-studied catalytic mechanism. However, little is known about the oligomeric state of this class of...
E Pourkarimi et al.
Cell death and differentiation, 19(3), 406-415 (2011-09-03)
In C. elegans, the BH3-only domain protein EGL-1, the Apaf-1 homolog CED-4 and the CED-3 caspase are required for apoptosis induction, whereas the Bcl-2 homolog CED-9 prevents apoptosis. Mammalian B-cell lymphoma 2 (Bcl-2) inhibits apoptosis by preventing the release of...
Hans Blom et al.
International journal of molecular sciences, 11(2), 386-406 (2010-04-14)
Electrostatic interactions between dielectric surfaces and different fluorophores used in ultrasensitive fluorescence microscopy are investigated using objective-based Total Internal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacial dynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein, zwitterionic rhodamine 110 and...
Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes.
Hennig, S.; et al.
Nano Letters, 15(2), 1374-1381 (2015)
Verbesserte hochauflosende Mikroskopie mit Oxazinfarbstoffen in schwerem Wasser.
Lee, S. F.; Verolet, Q.; Furstenberg, A.
Angewandte Chemie (International Edition in English), 124(34), 9117-9120 (2013)
Gražvydas Lukinavičius et al.
Current opinion in chemical biology, 15(6), 768-774 (2011-11-15)
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins...
Anna Kułakowska et al.
Journal of fluorescence, 20(2), 563-569 (2009-12-30)
In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching...
Claudio G Rolli et al.
PloS one, 5(1), e8726-e8726 (2010-01-22)
Cell migration is a fundamental feature of the interaction of cells with their surrounding. The cell's stiffness and ability to deform itself are two major characteristics that rule migration behavior especially in three-dimensional tissue. We simulate this situation making use...
Markus Hirsch et al.
Biological chemistry, 393(1-2), 23-35 (2012-05-26)
Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted...
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde, S.; et al.
Photochemistry and Photobiology, 10(4), 499-506 (2011)
Intrinsically Resolution Enhancing Probes for Confocal Microscopy.
Vogelsang, J.; et al.
Nano Letters, 10(2), 672-679 (2010)
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy.
Vogelsang, J.; et al.
Proceedings of the National Academy of Sciences of the USA, 106(20), 8107-8112 (2009)
Dynamic Submicroscopic Signaling Zones Revealed by Pair Correlation Tracking and Localization Microscopy.
You, Ch.; et al.
Analytical Chemistry, 86(17), 8593-8602 (2014)
Beatrice Dietz et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(31), 10699-10712 (2012-08-03)
In the developing nervous system, spontaneous neuronal activity arises independently of experience or any environmental input. This activity may play a major role in axonal pathfinding, refinement of topographic maps, dendritic morphogenesis, and the segregation of axonal terminal arbors. In...
Katarzyna Sołtys et al.
The Journal of steroid biochemistry and molecular biology, 198, 105571-105571 (2019-12-28)
The retinoid X receptor (RXR) is a member of the nuclear receptor (NR) superfamily that occupies the central position among other NRs by forming both homodimers and heterodimers with other representatives of the family. RXR shares similar structural domains with...
Hossein Tezval et al.
The Prostate, 69(4), 443-448 (2008-12-06)
Expression of urocortin (Ucn) in the human benign prostate and prostate cancer has been reported recently. Ucn binds and activates corticotropin releasing factor (CRF) receptor 1 (CRFR1) and 2 (CRFR2). Activation of CRFR2 has been shown to inhibit tumor growth...
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo, N. H.; et al.
The Journal of Cell Biology, 205(4), 591-606 (2014)
Fredrik Persson et al.
Nano letters, 12(5), 2260-2265 (2012-03-22)
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective...
Christian M Roth et al.
Analytical chemistry, 79(19), 7340-7345 (2007-09-07)
Current efforts to monitor the diffusion of proteins in living cells are based on either fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching, or image correlation spectroscopy. However, these methods cannot generate a map of diffusion times. Here, we introduce...
Jonas K Hannestad et al.
ACS nano, 7(1), 308-315 (2012-12-12)
We use single-molecule fluorescence microscopy to monitor individual hybridization reactions between membrane-anchored DNA strands, occurring in nanofluidic lipid monolayer films deposited on Teflon AF substrates. The DNA molecules are labeled with different fluorescent dyes, which make it possible to simultaneously...
Daniela C Dieterich
Current opinion in neurobiology, 20(5), 623-630 (2010-07-24)
Fluorescent proteins have revolutionized cell biology and, therefore, our understanding of the complex molecular and cellular mechanisms that wire the brain together and enable its plasticity throughout life. The ability to visualize cell biological processes has inspired the development of...
Anne Månsson et al.
Journal of leukocyte biology, 85(4), 719-727 (2009-01-09)
Viral respiratory infections are increasingly implicated in allergic exacerbations. The mechanisms behind this are not known, but a virus-induced activation of eosinophils through TLRs could be involved. Herein, we investigated the expression and function of TLR7 and TLR9 in purified...
Switching modulation for protein labeling with activatable fluorescent probes.
Kalyan K Sadhu et al.
Chembiochem : a European journal of chemical biology, 12(9), 1299-1308 (2011-06-02)
Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid.
Iserman, et al.
Molecular Cell, 80, 1078-1091 (2021)

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