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Determination of 7 Nitrosamines in Drinking Water

Nitrosamines analysis by solid phase extraction (SPE) and subsequent gas chromatography-tandem mass spectrometry (GC-MS/MS) according to GB 5750.10-2023.

Estimation of Proline in Fruit and Vegetable Juices

Spectrophotometric determination of proline by the Ninhydrin method, per EN 1141 and German Food and Feed Code §64 LFBG 31.00-7.

RNA Therapeutics & Delivery USA

Visit us at booth #7 to learn about our solutions to help you overcome your mRNA manufacturing and LNP formulation challenges.

Tumor Spheroid Formation Assay

3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.

cOmplete™, Mini, EDTA-free Protocol

Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.

Scepter™ 3.0 Cell Counter – How it Works

Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Assay of Betamethasone Sodium Phosphate by HPTLC

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

Ultrapure Water for Sensitive LC-MS Analysis of Pesticides

Pesticides analysis by LC-MS needs pesticide-free reagent water, which can be reliably delivered by Milli-Q® IQ 7 ultrapure water systems. See data.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

Obtaining Ultrapure Water for Sensitive PFAS Analysis by LC-MS

Sensitive PFAS testing can be performed with ultrapure water from a Milli-Q® IQ 7 system, which eliminates detectable PFAS for reliable LC-MS/MS results. See the science, see the proof.

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Paper-Bridge Loading

This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Tetrakis(dimethylamino)ethylene (TDAE)

In 2001, Professor William Dolbier, Jr., at the University of Florida reported1 an approach to nucleophilic trifluoromethylation based on the generation of a trifluoromethyl anion using CF3I in the presence of a powerful twoelectron reductant, tetrakis(dimethylamino)ethylene (TDAE)

Chemistry in (Bio)Materials Science

"Click" Chemistry in (Bio)Materials Science

Quantitative NMR for Content Assignment of Reference Standards for Quality Control of Herbal Medicinal Products

qNMR has proven to be appropriate as a primary analytical method for the certification of natural products as reference standards for QC of herbal medicinals.

What is Multi Column Chromatography?

Multi column chromatography has the benefit of using less resin, increasing productivity, and enabling continuous processing. Learn more in this technical article.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Sodium Triacetoxyborohydride

Sodium triacetoxyborohydride

Mesoporous Materials: Properties and Applications

Mesoporous Materials include a range of high surface area porous silicates with applications in gas adsorption, drug delivery, diagnostics and catalysis.

Chemical Synthesis of Monodisperse Magnetic Nanoparticles

Magnetic nanoparticles have attracted tremendous attention due to their novel properties and their potential applications in magnetic recording, magnetic energy storage and biomedicine.

Inverted Organic Photovoltaic Devices Using Zinc Oxide Nanocomposites as Electron Transporting Layer Materials

Organic photovoltaics (OPVs) represent a low-cost, lightweight, and scalable alternative to conventional solar cells. While significant progress has been made in the development of conventional bulk heterojunction cells, new approaches are required to achieve the performance and stability necessary to

Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility

Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique

Dendrons and Hyperbranched Polymers: Multifunctional Tools for Materials Chemistry

Dendrons in nanomaterials - Find features and benefits of polyester dendrons and hyperbranched polymer products.

Safer High-performance Electrodes, Solid Electrolytes, and Interface Reactions for Lithium-ion Batteries

Li-ion batteries are currently the focus of numerous research efforts with applications designed to reduce carbon-based emissions and improve energy storage capabilities.

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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