Showing 1-30 of 34 results for "46945"
Takayuki Morikawa et al.
Scientific reports, 10(1), 6422-6422 (2020-04-15)
Physiological regulation of blood flow in bone marrow is important to maintain oxygen and glucose supplies but also the physiological hypoxic state of the hematopoietic stem cell (HSC) niche. However, regulatory mechanisms underlying microcirculation in the bone marrow (BM) niche...
Fabiola A Sánchez et al.
The Journal of biological chemistry, 286(35), 30409-30414 (2011-07-16)
Endothelial NOS (eNOS)-derived NO is a key factor in regulating microvascular permeability. We demonstrated previously that eNOS translocation from the plasma membrane to the cytosol is required for hyperpermeability. Herein, we tested the hypothesis that eNOS activation in the cytosol...
Pradip Dey et al.
ACS nano, 12(7), 6429-6442 (2018-06-13)
The entry process of viruses into host cells is complex and involves stable but transient multivalent interactions with different cell surface receptors. The initial contact of several viruses begins with attachment to heparan sulfate (HS) proteoglycans on the cell surface...
Mabruka Alfaidi et al.
Journal of the American Heart Association, 9(11), e016099-e016099 (2020-05-30)
Background Alteration in hemodynamic shear stress at atheroprone sites promotes endothelial paracellular pore formation and permeability. The molecular mechanism remains unknown. Methods and Results We show that Nck (noncatalytic region of tyrosine kinase) deletion significantly ameliorates disturbed flow-induced permeability, and...
Luisa Bell et al.
Histochemistry and cell biology, 151(6), 489-499 (2019-01-23)
Recent studies with B-cell-depleting antibodies have demonstrated clinical success in the treatment of multiple sclerosis (MS) patients. While these antibodies efficiently target B cells in the blood, it is unclear how effective they are in the central nervous system (CNS)...
Tao Tan et al.
Nature communications, 10(1), 3322-3322 (2019-07-28)
The tumor stromal microenvironments (TSM) including stromal cells and extracellular matrix (ECM) form an abominable barrier hampering nanoparticles accessibility to cancer cells, significantly compromising their antitumor effects. Herein, we report a bioinspired lipoprotein (bLP) that can induce efficient photothermia to...
Silvia Huerta López et al.
Developmental biology, 444 Suppl 1, S337-S351 (2018-10-08)
Transcription factors that coordinate migration, differentiation or proliferation of enteric nervous system (ENS) precursors are not well defined. To identify novel transcriptional regulators of ENS development, we performed microarray analysis at embryonic day (E) 17.5 and identified many genes that...
Stefanie Wöhl-Bruhn et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 81(3), 573-581 (2012-05-15)
Hydrogels are promising delivery systems for the controlled release of biomacromolecules. Based on previous studies, hydrogels were prepared from crosslinkable hydroxyethyl starch with new linker groups to improve mechanical and release properties of the resulting hydrogels. Polyethylene glycol methacrylate with...
Timothy Fulton et al.
Current biology : CB, 30(15), 2984-2994 (2020-06-20)
A fundamental question in developmental biology is how the early embryo establishes the spatial coordinate system that is later important for the organization of the embryonic body plan. Although we know a lot about the signaling and gene-regulatory networks required...
Chuan He et al.
FEBS letters, 587(3), 285-290 (2012-12-25)
We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that...
Feng Li et al.
The Prostate, 79(11), 1226-1237 (2019-06-19)
We previously reported the presence of prostate-specific antigen (PSA) in the stromal compartment of benign prostatic hyperplasia (BPH). Since PSA is expressed exclusively by prostatic luminal epithelial cells, PSA in the BPH stroma suggests increased tissue permeability and the compromise...
Louise Coppin et al.
Cells, 8(8) (2019-08-10)
The efficacy of mesenchymal stem cell infusion is currently tested in numerous clinical trials. However, therapy-induced thrombotic consequences have been reported in several patients. The aim of this study was to optimize protocols for heterologous human adult liver-derived progenitor cell...
Misty R Riddle et al.
Developmental biology, 441(2), 285-296 (2018-06-09)
Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct...
Cătălin Voiniciuc et al.
Plant physiology, 169(1), 403-420 (2015-07-30)
Plants invest a lot of their resources into the production of an extracellular matrix built of polysaccharides. While the composition of the cell wall is relatively well characterized, the functions of the individual polymers and the enzymes that catalyze their...
Sandra Dittmar et al.
Journal of virology, 82(22), 11273-11282 (2008-09-05)
In order to analyze whether measles virus (MV) is transported via transmigrating leukocytes across endothelial barriers or whether virus spreads via infection of endothelial cells and basolateral release, we investigated the migratory behavior of infected human primary T lymphocytes across...
Mariana L Ferrari et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(27), 13582-13591 (2019-06-19)
Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type...
Veena Ammanathan et al.
Autophagy, 16(9), 1584-1597 (2019-11-21)
Macroautophagy/autophagy functions as a part of the innate immune system in clearing intracellular pathogens. Although this process is well known, the mechanisms that control antibacterial autophagy are not clear. In this study we show that during intracellular Salmonella typhimurium infection...
Cristina Bertulli et al.
Scientific reports, 8(1), 12480-12480 (2018-08-22)
With the push to reduce in vivo approaches, the demand for microphysiological models that recapitulate the in vivo settings in vitro is dramatically increasing. Here, we present an extracellular matrix-integrated microfluidic chip with a rounded microvessel of ~100 µm in diameter....
Andrew W Brown et al.
ChemMedChem, 13(24), 2618-2626 (2018-10-04)
The combretastatins have attracted significant interest as small-molecule therapies for cancer due to their ability to function as vascular disrupting agents. We have successfully prepared a range of combretastatin analogues that are based on a novel sydnone heterocycle core, and...
Christina T Echagarruga et al.
eLife, 9 (2020-10-06)
Cortical neural activity is coupled to local arterial diameter and blood flow. However, which neurons control the dynamics of cerebral arteries is not well understood. We dissected the cellular mechanisms controlling the basal diameter and evoked dilation in cortical arteries...
Neil Y C Lin et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(12), 5399-5404 (2019-03-06)
Three-dimensional renal tissues that emulate the cellular composition, geometry, and function of native kidney tissue would enable fundamental studies of filtration and reabsorption. Here, we have created 3D vascularized proximal tubule models composed of adjacent conduits that are lined with...
Joel W Blanchard et al.
Nature medicine, 26(6), 952-963 (2020-06-10)
In Alzheimer's disease, amyloid deposits along the brain vasculature lead to a condition known as cerebral amyloid angiopathy (CAA), which impairs blood-brain barrier (BBB) function and accelerates cognitive degeneration. Apolipoprotein (APOE4) is the strongest risk factor for CAA, yet the...
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss...
Siegfried Weisenburger et al.
Cell, 177(4), 1050-1066 (2019-04-16)
Calcium imaging using two-photon scanning microscopy has become an essential tool in neuroscience. However, in its typical implementation, the tradeoffs between fields of view, acquisition speeds, and depth restrictions in scattering brain tissue pose severe limitations. Here, using an integrated...
L. Cole et al.
Journal of Cell Science, 96, 721-721 (1990)
Use of horseradish peroxidase and fluorescent dextrans to study fluid pinocytosis in leukocytes.
J M Oliver et al.
Methods in enzymology, 108, 336-347 (1984-01-01)
Miguel Gaspar et al.
Cancer immunology research, 8(6), 781-793 (2020-04-11)
Following the success of immune checkpoint blockade therapy against cancer, agonistic antibodies targeting T-cell costimulatory pathways are in clinical trials. The TNF superfamily of receptors (TNFRSF) members CD137 and OX40 are costimulatory receptors that stimulate T-cell proliferation and activation upon...
S N Suresh et al.
Autophagy, 13(7), 1221-1234 (2017-03-30)
Parkinson disease (PD) is a life-threatening neurodegenerative movement disorder with unmet therapeutic intervention. We have identified a small molecule autophagy modulator, 6-Bio that shows clearance of toxic SNCA/α-synuclein (a protein implicated in synucleopathies) aggregates in yeast and mammalian cell lines....
Jungwook Paek et al.
ACS nano, 13(7), 7627-7643 (2019-06-14)
The vasculature is an essential component of the circulatory system that plays a vital role in the development, homeostasis, and disease of various organs in the human body. The ability to emulate the architecture and transport function of blood vessels...
Guangxiang Zang et al.
BMC cancer, 15, 234-234 (2015-04-18)
Shb is a signaling protein downstream of vascular endothelial growth factor receptor-2 and Shb deficiency has been found to restrict tumor angiogenesis. The present study was performed in order to assess metastasis in Shb deficiency using B16F10 melanoma cells. B16F10...

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