Showing 1-30 of 39 results for "47140"
Yasuhiko Higashi et al.
Biological & pharmaceutical bulletin, 29(12), 2479-2482 (2006-12-05)
The purpose of this study was to determine the level of 4-(4-bromophenyl)-4-hydroxypiperidine (BPHP), a bromperidol (BRO) metabolite, in rat plasma by HPLC with fluorescence detection after pre-column derivatization using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). After basic extraction of the samples with benzene, derivatization...
Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm...
Jing Luo et al.
Biochimica et biophysica acta, 1794(2), 335-340 (2008-11-26)
The heterodimeric 2-oxoacid:ferredoxin oxidoreductase (StOFOR) from Sulfolobus tokodaii, a thermoacidophilic archaeon, was inactivated by low concentrations of 4-fluoro-7-nitrobenzofurazan (NBD-F), with concomitant increase in fluorescence in subunit-b. The inactivation was prevented by CoA, suggesting that NBD-F covalently bound to the Lys...
Giuseppe Carlucci et al.
Biomedical chromatography : BMC, 29(6), 911-917 (2014-10-31)
A simple and sensitive method based on the combination of derivatization and high-performance liquid chromatography with ultraviolet and fluorimetric detection was developed for the simultaneous determination of octreotide and gabexate mesylate metabolite in human pancreatic juice samples. Parameters of the...
Hongyi Zhang et al.
Analytical and bioanalytical chemistry, 386(5), 1387-1394 (2006-09-06)
An in-capillary derivatization of amino acids and peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed for their subsequent capillary electrophoretic analysis with laser-induced fluorescence detection (lambda (ex)=488 nm). The in-capillary derivatization was achieved in zone-passing mode by introducing successive plugs of sample...
Laura E Brudecki et al.
Archives of biochemistry and biophysics, 471(2), 168-175 (2008-02-05)
The role of alphaPhe-291 residue in phosphate binding by Escherichia coli F1F0-ATP synthase was examined. X-ray structures of bovine mitochondrial enzyme suggest that this residue resides in close proximity to the conserved betaR246 residue. Herein, we show that mutations alphaF291D...
Bryan R Fonslow et al.
Analytical chemistry, 80(9), 3182-3189 (2008-03-21)
The continuous nature of micro free-flow electrophoresis (mu-FFE) was used to monitor the effect of a gradient of buffer conditions on the separation. This unique application has great potential for fast optimization of separation conditions and estimation of equilibrium constants....
Yasuhiko Higashi et al.
Journal of cosmetic science, 64(5), 381-389 (2013-10-22)
Hinokitiol, a potent, broad-spectrum antibacterial agent, is a component of various personal care products. In this study, the concentration of hinokitiol in skin lotion was analyzed by means of high-performance liquid chromatography-ultraviolet detection (380 nm) after precolumn derivatization with 4-fluoro-7-nitro-2,1...
João Mello-Vieira et al.
Biochimica et biophysica acta, 1828(9), 2152-2163 (2013-06-12)
Submillimolar concentrations of cytotoxic bile acids (BAs) induce cell death via apoptosis. On the other hand, several cytoprotective BAs were shown to prevent apoptosis in the same concentration range. Still, the mechanisms by which BAs trigger these opposite signaling effects...
Takato Abe et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 34(12), 1928-1935 (2014-10-09)
D-Serine is known to be essential for the activation of the N-methyl-D-aspartate (NMDA) receptor in the excitation of glutamatergic neurons, which have critical roles in long-term potentiation and memory formation. D-Serine is also thought to be involved in NMDA receptor-mediated...
XiFeng Wang et al.
Biomedical and environmental sciences : BES, 24(5), 537-542 (2011-11-24)
A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples, including plasma, brain, and liver. The optimum derivatization reaction temperature was 70 °C, and at this temperature...
Zsolt Wagner et al.
Electrophoresis, 32(20), 2816-2822 (2011-10-20)
Three different fluorescent tags have been compared for the quantitative analysis of aspartate and glutamate in brain microdialysate samples. Separation conditions have been optimized to achieve short analysis time using reversed polarity separation in coated capillary. Method validation has revealed...
Yurika Miyoshi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 966, 187-192 (2014-03-04)
Enantioselective amino acid metabolome analysis of the Japanese traditional black vinegars (amber rice vinegar, Kurozu) was performed using two-dimensional high-performance liquid chromatography combining a microbore-monolithic ODS column and narrowbore-enantioselective columns. d-Amino acids, the enantiomers of widely observed l-amino acids, are...
Hua-Ming Tseng et al.
Analytical and bioanalytical chemistry, 388(2), 433-439 (2007-03-21)
A rapid and sensitive capillary electrophoresis (CE) method has been developed for profiling organic metabolites containing amine functional groups in mammalian biofluids. Metabolites containing an amine group were derivatized with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazol (NBD-F), separated by micellar electrokinetic chromatography (MEKC), and detected...
Chanda Ciriacks Klinker et al.
Analytical chemistry, 79(22), 8747-8754 (2007-10-13)
4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) has been characterized as a fluorogenic labeling reagent for high speed, online microdialysis-capillary electrophoresis (MD-CE) assays for amino acid neurotransmitters. NBD-F labeled amines are efficiently excited using 488 nm light allowing more common lasers to be used for...
Guanmin Su et al.
Journal of food science, 76(9), N74-N78 (2012-03-16)
This article presents an improved method to detect D-glucosamine hydrochloride in health foods. A simple precolumn derivatization procedure with 7-flouro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) reagent was employed. The separation of the derivatized D-glucosamine hydrochloride (NBD-D-glucosamine hydrochloride) was performed using a mobile phase consisting...
Hai Han et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(29), 3196-3202 (2011-03-05)
For a metabolomics study focusing on the analysis of aspartic and glutamic acid enantiomers, a fully automated two-dimensional HPLC system employing a microbore-ODS column and a narrowbore-enantioselective column was developed. By using this system, a detailed distribution of D-Asp and...
T Yagi et al.
Journal of clinical pharmacy and therapeutics, 37(1), 89-94 (2011-02-01)
A rapid derivatization and validated HPLC method for gabapentin in human plasma and urine is needed for clinical use. The objective of this study was to establish a rapid and validated analytical method for the determination of gabapentin in human...
Yang-Ki Park et al.
Journal of chromatography. A, 1217(20), 3357-3361 (2010-04-13)
Single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis by attaching a drop to the tip of a capillary. With a 2-layer drop comprised of an aqueous basic acceptor phase covered with a thin organic layer, acidic analytes...
Yanting Song et al.
Amino acids, 42(5), 1897-1902 (2011-04-21)
A high-performance liquid chromatography method in which fluorescence detection is used for the simultaneous determination of 21 amino acids is proposed. Amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and then separated on a monolithic silica column (MonoClad C18-HS, 150 mm×3...
Chiaki Aoyama et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(1), 63-65 (2009-01-14)
A simple and sensitive determination method for selenomethionine (Se-Met) using an HPLC-fluorescence detection system coupled with an on-line electrochemical reactor has been developed. NBD-F (4-fluoro-7-nitro-2,1,3-benzoxadiazole) was used as the fluorescent derivatization reagent for Se-Met. NBD-Se-Met was separated from NBD-derivatives of...
Danielle Miller et al.
Biochemistry, 53(28), 4635-4647 (2014-07-01)
We have established the biosynthetic pathway and the associated genes for the biosynthesis of the 5-(aminomethyl)-3-furanmethanol (F1) moiety of methanofuran in the methanogenic archaeon Methanocaldococcus jannaschii. The recombinant enzyme, derived from the MJ1099 gene, was shown to readily condense glyceraldehyde...
Gabriel E Romero et al.
ACS chemical neuroscience, 5(9), 848-854 (2014-08-02)
D-Serine, an N-methyl D-aspartate receptor coagonist, and its regulatory enzymes, D-amino acid oxidase (DAO; degradation) and serine racemase (SR; synthesis), have been implicated in crucial roles of the developing central nervous system, yet the functional position that they play in...
Yaru Song et al.
Analytical chemistry, 78(23), 8121-8128 (2006-12-01)
A sensitive chiral capillary HPLC-MS/MS method well suited for the determination of amino acid enantiomers in biological samples was developed. The method involved precolumn derivatization of the sample with 7-fluoro-4-nitrobenzoxadiazole (NBD-F). After derivatization, NBD-amino acids were stacked on a C18...
Yasuhiko Higashi et al.
Journal of cosmetic science, 63(3), 205-212 (2012-12-01)
Various methods for the determination of kojic acid (KA), a skin-whitening agent, have been reported by high-performance liquid chromatography (HPLC). In this study, the concentration of KA in a skin-whitening cosmetic was analyzed by HPLC with ultraviolet detection (380 nm)...
Mostafa A Shehata et al.
Journal of AOAC International, 89(3), 646-650 (2006-06-24)
A simple, accurate, precise, and sensitive kinetic spectrophotometric method for determination of befunolol hydrochloride is described. The method is based on the formation of a colored product with 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) in methanol at 70 degrees C for 45 min. The...
Xiangtang Li et al.
Journal of chromatography. A, 1285, 159-164 (2013-03-12)
Integration of a pressure-driven make-up flow (MUF) into a microchip electrophoresis (MCE) platform in order to facilitate its coupling with electrospray ionization-mass spectrometric detection (ESI-MS) is described. In the glass/PDMS hybrid microchip, a MUF channel was made to intersect with...
Carmen L Delgado et al.
Biochimica et biophysica acta, 1848(4), 895-906 (2015-01-03)
In a previous study, it was shown that purified preS domains of hepatitis B virus (HBV) could interact with acidic phospholipid vesicles and induce aggregation, lipid mixing and leakage of internal contents which could be indicative of their involvement in...
Xifeng Wang et al.
Journal of chromatographic science, 50(2), 119-122 (2012-02-03)
A rapid and highly sensitive high-performance liquid chromatograpy method with fluorescence detection has been developed for determination of glutathione (GSH) in human plasma. A simple pre-column derivatization procedure with 7-flouro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) reagent was employed. The separation of the derivatized glutathione...
Ryuki Miyauchi et al.
Phytochemistry, 116, 87-93 (2015-04-14)
Nectrisine, an iminosugar with a heterocyclic nitrogen-containing 5-membered ring, acts as a glycosidase inhibitor. Thelonectria discophora SANK 18292, a fungus, was identified as a nectrisine producer from its microbial library in our screening for nectrisine producing microorganisms. Biosynthesis of nectrisine...

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