HCK (230-497) Active (H2915) - Datasheet
HCK (230-497) Active
human, recombinant
GST-tagged, expressed in Sf9 cells
Catalog Number H2915
Lot Number 118K0533
Storage Temperature –70 °C
Synonyms
retinoblastoma protein in
estradiol-treated MCF-7 human breast cancer
cells. Biochem. Biophys. Acta, 497:
186-196 (2000).
2. Kimura, N., et al., BMP2-induced apoptosis is
mediated by activation of the
1988). Dev. Neuroscience 10:222-230.
20. Ruoslahti, E. and Pierschbacher, M. (1987). Science 238:491-497.
21. Pierschbacher, M. and Ruoslahti, E. (1987). J. Biol. Chem. 262(36):17294-17298.
22. Cappello
al., Advances in Refolding of Proteins
Produced in E. Coli. Current Opinion in
Biotechnology, 9, 497-501 (1998).
8. Marston, F.A.O., and Hartley, D.L., Solubilization of
Protein Aggregates. Methods
10/14-1
OD 450 nm Concentration (µIU/mL)
Standard 1 .025 0
Standard 2 .096 3
Standard 3 .223 6
Standard 4 .497 15
Standard 5 1.194 35
Standard 6 2.095 70
2014 Sigma-Aldrich Co. LLC.
1981).
Goyert, S. M., et al., J. Immunol., 137, 3909 (1986).
Goyert, S. M., et al., Science, 239, 497 (1988).
JWM/KMR 08/02
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
cAMP-dependent protein
kinase inhibits signaling through the T cell receptor.
J. Exp. Med., 193, 497-507 (2001).
8. Benistant, C., et al., The COOH-terminal Src kinase
Csk is a tumor antigen in human
1988). Dev. Neuroscience 10:222-230.
20. Ruoslahti, E. and Pierschbacher, M. (1987). Science 238:491-497.
21. Pierschbacher, M. and Ruoslahti, E. (1987). J. Biol. Chem. 262(36):17294-17298.
22. Cappello
, Clin. Exp. Immunol., 43, 497
(1981).
Garson, J. A., et al., Nature, 298, 375 (1982).
Burns, G. F., et al., J. Immunol., 129, 1451 (1982).
Reinherz, E. L., et al., Cell, 30, 735 (1982).
Borst, J., et
vagal complex : an immunocytochemical study by light and election microscopy. Brain Res., (1989),
497, 260-270.
6) MONS N., TISON F. and GEFFARD M. Existence of L-DOPA immunoreactive neurons in
Johnson,
R. (ed.), Raven Press, New York, p 181 (1987).
2. Gregory, R., Bioorg. Chem., 8, 497 (1979).
3. Larsson, L., et al., Scand. J. Gastroenterol., 12, 869
(1977).
4. Wool, P., Br. J. Cancer
R. E. Callard, Eur. J. Immunol.
11, 329 (1981).
Callard, R. E., et al., Clin. Exp. Immunol., 43, 497
(1981).
Garson, J. A., et al., Nature, 298, 375 (1982).
Burns, G. F., et al., J. Immunol., 129, 1451
R., Advances
in Refolding of Proteins Produced in E. Coli.
Current Opinion in Biotechnology. 9, 497-501
(1998).
7. Marston, F.A.O. and Hartley, D.L., Solubilization of
Protein Aggregates. Methods
non-specific
mouse immunoglobulin at the same
concentration as test antibody followed by
steps 3 - 8.
3. After 30 minutes add 2 ml of diluent to all tubes.
4. Pellet cells by centrifugation at 500 x g, for
1981).
Goyert, S. M., et al., J. Immunol., 137, 3909 (1986).
Goyert, S. M., et al., Science, 239, 497 (1988).
Haziot, A., et al., J. Immunol., 141, 547 (1988).
Hogg, N., et al., Immunology, 53, 753 (
Invest., 68, 932 (1981).
3. Todd, R. F., et al., J. Immunol., 126, 1435 (1981).
4. Goyert, S. M., et al., J. Immunol., 137, 3909
(1986).
5. Goyert, S. M., et al., Science, 239, 497 (1988).
6. Haziot, A.
retinoblastoma protein in
estradiol-treated MCF-7 human breast cancer cells.
Biochem. Biophys. Acta, 497, 186-196 (2000).
9. Kimura, N. et al., BMP2-induced apoptosis is
mediated by activation of the