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X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

8th CAR-TCR Summit 2023

Visit booth 38 to learn about VirusExpress lentivirus platform and de-risk lentivirus production for cell and gene therapies.

MilliporeSigma Expands in Gillingham, UK, Boosts Distribution Capabilities

MilliporeSigma today announced the completion of a £9 million expansion to its Gillingham distribution center.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

Accelerated mAb Development Program – From Transfection to GMP Drug Substance in 9 Months

The accelerated mAb development program delivers GMP drug substance nine months from transfection. We designed the mAbExpress™ program to be fast, cost effective, and appropriate for the early clinical phase of your program, with regulatory support throughout.

How to Boost AAV Titer with Feed Supplementation in both HEK293 and Sf-9 Platforms

In this webinar, you will learn wow to boost AAV titers in HEK293 and Sf-9 cell lines as well as getting some insider tips on volume and timing optimization.

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Analysis of 17 Cannabinoids in Hemp and Cannabis

HPLC separation of 17 important cannabinoids including CBD, delta 9 THC and THCA. Read the application note.

Column Packing and Preparation for Size Exclusion Chromatography

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Determination of Phosphorus Total in Meat and Meat Products

Colorimetric total phosphorus analysis method for meat and meat products as per German Food and Feed Code §64 LFGB 06.00-9.

RESOMER® - Biodegradable Polymers for Sutures, Medical Devices, Drug Delivery Systems and Tissue Engineering

Interest in utilizing biodegradable polymers for biomedical applications has grown since the 1960s.

Identification of fixed oils by Thin layer chromatography

This application note describes the HPTLC-based identification of fixed oils using octadecyl silyl silica gel as the coating substance, following USP 43 NF 38 monograph method containing USP general chapter .

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH

Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.

Analysis of Antioxidants in Bakery Products

HPLC-UV method detects 9 antioxidants like propyl gallate in food using a Discovery®</sup HS C18 column for excellent peak shapes.

GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol

GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA

Secondary Antibodies, Conjugates, and Kits

Technical Support for Secondary Antibodies, Conjugates, and Kits

Quantum Dots for Electronics and Energy Applications

In this article, the properties of some of the new non-cadmium based QDs along with different applications of QDs are summarized.

Flavors and Fragrances New Product Offerings

As a leading global supplier of new flavor and fragrance ingredients, we are dedicated to providing you with high-quality aroma chemicals comprising of certified food grade, IFRA, natural, kosher, and halal ingredients.

Surface-Enhanced Solar Energy Conversion Systems

Sustainable energy sources with high production efficiency are crucial for meeting increasing energy demand.

EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA)

EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection

Virus Cultivation

Cell lines for vaccine production and cultivation of SARS CoV-2 and other viruses. Primary, human diploid, and continuous culture cell lines for the propagation of viruses and virus-based vaccines.

Revolution in Plastic Recycling: Laser Marking & Digital Product Passports Enable True Circular Economy

An industry-wide project demonstrates how packaging can become smarter, more sustainable, and fully traceable. Plastics are a blessing—and a global problem. Lightweight, durable, and versatile. Yet these very properties make plastics a challenge worldwide. Over 400 million tons are produced

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Epigenetics Overview

Epigenetics is a term coined to describe changes that are not mutation based but can still be passed on from generation to generation. Genes that are activated or repressed without any change in DNA sequence are epigenetically controlled. Epigenetic modifications

Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads

This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.

2D MXenes Ink: Synthesis, Properties, and Their Precursors

Optimizing the synthesis of high-quality 2D MXene flakes for 3D ink printing is essential to such technological developments as printable and flexible electronics.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

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