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David Pantoja-Uceda et al.
Journal of magnetic resonance (San Diego, Calif. : 1997), 234, 190-196 (2013-07-31)
A suite of (13)C-detected NMR pulse sequences to edit the correlation peaks of the CACO and CON spectra according to the amino acid residue type is presented. The pulse sequences exploit the topology of the C(β) carbon and led to
Demidmaa Tuvdendorj et al.
Metabolism: clinical and experimental, 63(12), 1562-1567 (2014-10-14)
The use of stable isotope tracer techniques to measure muscle protein fractional synthesis rate (FSR) has been well established and widely used. The most common method that has been utilized so far is a primed constant infusion (CI) method, which
O V Mosin et al.
Prikladnaia biokhimiia i mikrobiologiia, 49(3), 255-266 (2013-07-26)
A 2H-labeled purine ribonucleoside inosine was microbiologically synthesized (yield, 3.9 g/L of culture liquid) using a deuterium-adapted strain ofthe gram-positive chemoheterotrophic bacterium Bacillus subtilis, cultivated in a heavy water medium with a high degree of deuteration (99.8 at % 2H)
Martin Berggren et al.
Ecology, 95(7), 1947-1959 (2014-08-29)
The importance of terrestrial-derived organic matter for lake zooplankton communities remains debated, partly because little is known about the basic pathways by which allochthonous carbon is transferred to zooplankton, and whether these vary among the major taxonomic and functional groups.
Alberto Sánchez-Guijo et al.
Journal of lipid research, 56(9), 1843-1851 (2015-08-05)
Steroids are primarily present in human fluids in their sulfated forms. Profiling of these compounds is important from both diagnostic and physiological points of view. Here, we present a novel method for the quantification of 11 intact steroid sulfates in
Norbert Gleicher et al.
Reproductive biology and endocrinology : RB&E, 13, 38-38 (2015-05-07)
Low functional ovarian reserve (LFOR) has been associated with hypoandrogenemia and increased embryo aneuploidy, while androgen supplementation has been reported to improve aneuploidy rates. We, therefore, assessed whether in infertile women undergoing in vitro fertilization (IVF) androgen concentrations are associated
Cécile Meunier et al.
Clinica chimica acta; international journal of clinical chemistry, 447, 8-15 (2015-05-20)
Accurate quantitation of aldosterone is essential for screening, diagnosis and subtype classification in primary aldosteronism. A simple, sensitive method for aldosterone in human plasma using supported liquid extraction (SLE) in combination with liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed
Jose Luis Muñoz-Muñoz et al.
IUBMB life, 65(9), 793-799 (2013-07-31)
A solvent deuterium isotope effect on the inactivation suicide of tyrosinase in its action on o-diphenols (catechol, 4-methylcatechol, and 4-tert-butylcatechol) was observed. This isotope effect, observed during kinetic studies in the transition phase, was higher than that described previously in
Russell J Chedgy et al.
Phytochemistry, 113, 149-159 (2015-01-07)
Salicinoids are phenolic glycosides (PGs) characteristic of the Salicaceae and are known defenses against insect herbivory. Common examples are salicin, salicortin, tremuloidin, and tremulacin, which accumulate to high concentrations in the leaves and bark of willows and poplars. Although their
Ana Y Mercedes-Camacho et al.
Biochemistry, 52(44), 7707-7713 (2013-10-15)
The Pin1 peptidyl-prolyl isomerase catalyzes isomerization of pSer/pThr-Pro motifs in regulating the cell cycle. Peptide substrates, Ac-Phe-Phe-phosphoSer-Pro-Arg-p-nitroaniline, were synthesized in unlabeled form, and with deuterium-labeled Ser-d3 and Pro-d7 amino acids. Kinetic data were collected as a function of Pin1 concentration
Michael G Mazzacavallo et al.
PloS one, 10(12), e0144300-e0144300 (2015-12-04)
Root biomass distributions have long been used to infer patterns of resource uptake. These patterns are used to understand plant growth, plant coexistence and water budgets. Root biomass, however, may be a poor indicator of resource uptake because large roots
Emad Ehtesham et al.
Journal of agricultural and food chemistry, 61(37), 8914-8923 (2013-08-21)
The aim of this study was to investigate the correlation of δ²H and δ¹³C of bulk milk powder and milk powder fatty acids to their production region. A total of 46 milk powder samples from across New Zealand were collected
Demidmaa Tuvdendorj et al.
American journal of physiology. Endocrinology and metabolism, 305(8), E999-E1006 (2013-08-29)
The purpose of this study was to evaluate the variability of subcutaneous abdominal adipose tissue (AT) dynamics in obese subjects with a wide range of insulin sensitivity (IS) and the correlation between these two metabolic measures. Ten obese (BMI 30-40
Seung Bin Baek et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(46), 14156-14161 (2015-11-19)
Crystallographic observation of adsorbed gas molecules is a highly difficult task due to their rapid motion. Here, we report the in situ single-crystal and synchrotron powder X-ray observations of reversible CO2 sorption processes in an apparently nonporous organic crystal under
Shaogang Chu et al.
Analytica chimica acta, 885, 183-190 (2015-08-02)
A fast, robust and highly sensitive analysis method for determination of trace levels of organophosphate ester (OPE) flame retardants and plasticizers in lipid-rich samples was presently developed, and based on ultra-high performance liquid chromatography-tandem mass spectrometry coupled to a positive
Xiaoguang Sunny Li et al.
Analytical and bioanalytical chemistry, 406(28), 7287-7297 (2014-09-18)
Catecholamines are a class of biogenic amines that play an important role as neurotransmitters and hormones. We developed and validated a rapid, specific and sensitive LC-MS/MS method for quantitative determination of catecholamines in human urine. Linearity, specificity, sensitivity, precision, accuracy
Kim H Hee et al.
Journal of pharmaceutical and biomedical analysis, 102, 253-260 (2014-12-03)
Rifampicin (RIF) and isoniazid (INH), first line drugs for the treatment of tuberculosis, are known to cause hepatotoxicity as a serious adverse side effect. To further understand the pharmacokinetic parameters of these two drugs, we have developed and validated a
Brandon K Tate et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 21(28), 10160-10169 (2015-06-11)
Alkoxide-bridged disilver cations react with dihydrogen to form hydride-bridged cations, releasing free alcohol. Hydrogenolysis of neutral silver fluorides affords hydride-bridged disilver cations as their bifluoride salts. These reactions proceed most efficiently when the supporting ligands are expanded N-heterocyclic carbenes (NHCs)
Use of deuterium labeling in NMR: overcoming a sizeable problem.
M Sattler et al.
Structure (London, England : 1993), 4(11), 1245-1249 (1996-11-15)
Justin Jankunas et al.
The Journal of chemical physics, 138(9), 094310-094310 (2013-03-15)
The Photoloc technique has been employed to measure the state-resolved differential cross sections of the HD(v', j(')) product in the reaction H + D2 over a wide range of collision energies and internal states. The experimental results were compared with
Edward J Cone et al.
Journal of analytical toxicology, 39(3), 192-202 (2015-01-16)
Oxycodone (OC) is recommended to be included as an analyte tested in the proposed Substance Abuse and Mental Health Services Administration (SAMHSA's) Mandatory Guidelines for Federal Workplace Drug Testing Programs using Oral Fluid (OF) Specimens. This study demonstrates the time
Julia Homann et al.
Journal of chromatography. A, 1360, 150-163 (2014-08-16)
Pro-resolving lipid mediators are a class of endogenously synthesized molecules derived from different fatty acids, such as arachidonic, docosahexaenoic or eicosapentaenoic acid, which are derived into four different product families: lipoxins, resolvins, maresins and protectins. For quantitation of these compounds
Juan Zhao
The Journal of chemical physics, 138(13), 134309-134309 (2013-04-12)
We investigated spin-orbit-induced intersystem crossing effects in the title reaction by the time-dependent wave-packet method combined with an extended split operator scheme. We performed non-adiabatic calculations of the fine-structure-resolved cross section and adiabatic calculations of integral cross section. The calculations
Kimie Nakagawa et al.
PloS one, 9(8), e104078-e104078 (2014-08-16)
UbiA prenyltransferase domain containing 1 (UBIAD1) is a novel vitamin K2 biosynthetic enzyme screened and identified from the human genome database. UBIAD1 has recently been shown to catalyse the biosynthesis of Coenzyme Q10 (CoQ10) in zebrafish and human cells. To
Yashvin Neehaul et al.
Biochemistry, 52(18), 3085-3093 (2013-04-10)
The Na(+)-pumping NADH:quinone oxidoreductase (Na(+)-NQR) is a unique respiratory enzyme that conserves energy by translocating Na(+) through the plasma membrane. Found only in prokaryotes, the enzyme serves as the point of entry of electrons into the respiratory chain in many
Stefan Salentinig et al.
Chemistry and physics of lipids, 190, 43-50 (2015-07-08)
Medium chain triglycerides (MCTs) are a unique form of dietary fat that have a wide range of health benefits. They are molecules with a glycerol backbone esterified with medium chain (6-12 carbon atoms) fatty acids on the two outer (sn-1
Doubly labeled water is a validated and verified reference standard in nutrition research.
Maciej S Buchowski
The Journal of nutrition, 144(5), 573-574 (2014-02-28)
D A Dempsey et al.
Clinical pharmacology and therapeutics, 94(3), 400-406 (2013-05-30)
The formation of cotinine, the main proximate metabolite and a biomarker of nicotine exposure, is mediated primarily by cytochrome P450 (CYP)2A6. Our aim was to determine whether higher cotinine levels in young children exposed to secondhand smoke (SHS) are a
Lian-Jun Bao et al.
Environmental toxicology and chemistry, 32(9), 1946-1953 (2013-05-11)
Solid-phase microextraction (SPME) has often been used to estimate the freely dissolved concentration (Cfree ) of organic contaminants in sediments. A significant limitation in the application of SPME for Cfree measurement is the requirement for attaining equilibrium partition, which is
Ruibing Chen et al.
Journal of proteome research, 13(12), 5767-5776 (2014-09-13)
Temperature changes influence the reaction rates of all biological processes, which can pose dramatic challenges to cold-blooded organisms, and the capability to adapt to temperature fluctuations is crucial for the survival of these animals. In order to understand the roles
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