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620-12-2

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Data Sheet - 67351
Page 2 of 2 Directions for labeling of proteins with Atto 620 NHS ester Dissolve protein in of bicarbonate buffer (0.1 M, preferably of pH 8.3) at 2 mg/ml. Concentrations below 2 mg protein
Product Information Sheet - ADD641C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD621C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD620C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD670C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - HPR116080
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD640C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD671C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD650C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD651C
Maintenance/Metabolism Medium 620. For 12-24-48-96 well plate(s): - Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or 12 well plate, 9.6 mL/48 or 96
Data Sheet - L3790
1989). 5. Haas, M.J., et al., Methods Enzymol., 43, 620-628 (1975). 6. Schutte, H. et al., Biotechnology and Applied Biochemistry, 12, 599-620 (1990). 7. Rupley, J.A., Biochim. Biophys. Acta, 83
Product Information Sheet - L7651
14. Schutte, H., et al., Biotechnology and Applied Biochemistry, 12, 599-620 (1990). 15. Haas, M.J., et al., Methods in Enzymology, 43, 620-628 (1975). 16. Alderton, G., et al., Journal of Biological Chemistry
Product Information Sheet - PTP101
absorbance of 96-well plates at wavelength of 620-630 nm, or spectrophotometer (UV/Visible). 2. Incubator 30EC (If required). 3. Precision pipettes (2-20, 20-100 and 200-1000 :l) with disposable tips
Data Sheet - S5814
Procedure This product is used to stop TMB Microwell substrate and change the absorbance from blue (620 nm) to yellow (450 nm). Equal volumes of substrate and stop solution should be used. The resulting
Data Sheet - T1693
product forms that may be read at 370 nm or between 620 and 655 nm. For end-point assays, the reaction can be stopped by the addition of a volume of 1 or 2 N hydrochloric acid or 1 N...
Data Sheet - S5689
Liquid Substrate Systems (Catalog Numbers T5569, T4319, T8665, T0440, and T4444) in the blue range of 620 nm to 650 nm. Several publications cite use of this reagent in their protocols. 1,2
Corning Synthemax-R Surface for Research
Free Fax: 0800 022 9089 Tel: (+31) 78 620 5411 Fax: (+31) 78 620 5421 New Zealand Free Tel: 0800 936 666 Free Fax: 0800 937 777 Tel: (+61) 2 9841 0555 Fax: (+61) 2 9841 0500 Norway Tel: (+47) 23 17 60 00
Product Information Sheet - P7012
. Malamud, D., and Drysdale, J.W., Isoelectric Points of Proteins: A Table, Anal. Biochem., 86(2), 620-647 (1978). 4. Proc. Natl. Acad. Sci., 45, 915-922 (1959). 5. Enzymes of Molecular Biology
Product Information Sheet - P6887
3. Malamud, D., and Drysdale, J.W., Isoelectric Points of Proteins: A Table, Anal. Biochem., 86, 620-647 (1978). 4. Proc. Natl. Acad. Sci., 45, 915-922 (1959). 5. Sweeney, P.J., and Walker, J.M., in
Data Sheet - SAB4200657
cells. Immunofluorescence: a working concentration of 0.5-1 g/mL is recommended using SW-620 cells. Note: In order to obtain the best results using various techniques and preparations, we recommend
Product Information Sheet - A8200
Structure, 2(4), 283-291 (1994). 14. Greenblatt, H.M., et al., Streptomyces griseus aminopeptidase: X-ray crystallographic structure at 1.75 Å resolution. J. Mol. Biol., 265(5), 620-636 (1997)
Data Sheet - SAB4200465
Immunofluorescence: A working concentration of 2.5–5.0 g/mL is recommended using SW-620 cells. Note: In order to obtain the best results using various techniques and preparations
Product Information Sheet - N8130
indicators: determination of kinetic parameters by dynamic pH monitoring. Biotechnol. Bioeng., 72(6), 620-627 (2001). 6. Bazzicalupi, C., et al., CO2 fixation by novel copper(II) and zinc(II)
Acetylcholinesterase (C2888)
Acetylcholinesterase II. Crystallization, absorption spectra, isoionic point. Proc. Natl. Acad. Sci. USA, 59(2), 620-623 (1968). 13. Golicnik, M., and Stojan, J., Multi-step analysis as a tool for kinetic parameter
Data Sheet - SAB4200838
et al., Nat. Cell Biol., 17, 893-906 (2015). 6. Wan, Y., et al., Cell Mol. Immunol., 14, 607-620 (2017). VS,DR,OKF,MAM 07/19-1 2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
"MONOCLONAL ANTI-m-CALPAIN (DOMAIN III), CLONE 9A4H8D3 Mouse Ascites Fluid"
calmodulin, or calpastatin. Epitope mapping studies indicate the epitope is between amino acids 465-620 (domain III) of human µ-calpain. The calpain (Ca2+-dependent proteinase or Ca2+-activated neutral
Data Sheet - SRP5294
densitometry) Figure 2. Specific Activity of Typical Lot: 8–12 nmole/min/mg Procedure Preparation Instructions Kinase Assay Buffer – 25 mM MOPS, pH 7. 2, 12.5 mM glycerol 2-phosphate, 25 mM MgC12
Data Sheet - G2791
et al., Science, 266, 1241-1247 (1996). 5. Buday, L., and Downward, J., Cell, 73, 611-620 (1993). 6. Cheng, A.M., et al., Cell, 95, 793-803 (1998). 7. Schlesinger, J., Curr. Opin....
Product Information Sheet - MAK307
by adding 20 L of the Working Reagent to 80 L of sample solution. The blank OD values at 620 nm should be 0.2. If the OD readings are 0.2, check water...
Data Sheet - SAB4200416
discarded if not used within 12 hours. Product Profile Immunoblotting: a working concentration of 0.5-1.0 g/mL is recommended using extracts of SW-620 cells. Immunofluorescence