620-12-2
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Showing 1-30 of 308 results for "620-12-2" within Technical Documents
Product Information Sheet - ADD641C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD621C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Data Sheet - 67351
Page 2 of 2
Directions for labeling of proteins with Atto 620 NHS ester
Dissolve protein in of bicarbonate buffer (0.1 M, preferably of pH 8.3) at 2 mg/ml. Concentrations
below 2 mg protein
Product Information Sheet - ADD650C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD640C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD671C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD651C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - HPR116080
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD670C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
Product Information Sheet - ADD620C
Maintenance/Metabolism Medium 620.
For 12-24-48-96 well plate(s):
- Pre-warm the HepaRG® Maintenance/Metabolism Medium 620 in a sterile container (12 mL/24 or
12 well plate, 9.6 mL/48 or 96
171202 - Thermoreactor TR 620 - Operating manual
What to do if...
03/2020 33
TR 620
8 Technical Data
Reactor type Dry temperature control
device with safety cover
Cell shafts 2 x 12 cell shafts for
Product Information Sheet - MAK113
density (OD) of all wells at
620 nm.
Results
1. Create a standard curve by plotting the
OD values of the Standards against their
respective concentrations.
2. Calculate ΔOD values by subtracting
Product Information Sheet - L7651
14. Schutte, H., et al., Biotechnology and Applied Biochemistry, 12, 599-620 (1990).
15. Haas, M.J., et al., Methods in Enzymology, 43, 620-628 (1975).
16. Alderton, G., et al., Journal of Biological Chemistry
Product Information Sheet - PTP101
absorbance of 96-well plates at wavelength of 620-630 nm, or
spectrophotometer (UV/Visible).
2. Incubator 30EC (If required).
3. Precision pipettes (2-20, 20-100 and 200-1000 :l) with disposable tips
Data Sheet - S5814
Procedure
This product is used to stop TMB Microwell substrate
and change the absorbance from blue (620 nm) to
yellow (450 nm). Equal volumes of substrate and stop
solution should be used. The resulting
Data Sheet - T1693
product forms
that may be read at 370 nm or between 620 and 655
nm. For end-point assays, the reaction can be stopped
by the addition of a volume of 1 or 2 N hydrochloric acid
or 1 N
Corning Synthemax-R Surface for Research
Free Fax: 0800 022 9089
Tel: (+31) 78 620 5411
Fax: (+31) 78 620 5421
New Zealand
Free Tel: 0800 936 666
Free Fax: 0800 937 777
Tel: (+61) 2 9841 0555
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Data Sheet - S5689
Liquid Substrate Systems (Catalog Numbers
T5569, T4319, T8665, T0440, and T4444) in the blue
range of 620 nm to 650 nm.
Several publications cite use of this reagent in their
protocols.
1,2
Quality and Supply Chain Transparency Guidelines
Free Fax: +01 800 712 9920
Email: mexico@sial.com
The Netherlands
Tel: +31 (0)78 620 5414
Fax: +31 (0)78 620 5424
Free Tel: +0800 022 9092
Free Fax: +0800 022 9093
Email: nldsaf@sial.com
New
Product Information Sheet - S5689
Liquid
Substrate Systems (Cat. Nos. T5569, T4319, T8665,
T0440, and T4444) in the blue range of 620 nm to
650 nm. Several publications cite use of S5689 in
their protocols.1,2
Precautions and Disclaimer
Human E-Selectin ELISA Kit
microwell strips are stored at 2° to 8°C in the dark.
o. Read absorbance of each microwell on a spectrophotometer using 450 nm
as the primary wavelength (optionally 620 nm as the reference wavelength
Human ICAM-1 ELISA Kit
microwell strips are stored at 2° to 8°C in the dark.
o. Read absorbance of each microwell on a spectrophotometer using 450 nm
as the primary wavelength (optionally 620 nm as the reference
wavelength
Product Information sheet - S5814
Procedure
This product is used to stop TMB Microwell substrate
and change the absorbance from blue (620 nm) to
yellow (450 nm).
• Equal volumes of substrate and stop solution
should be used.
Data Sheet - SAB4200657
cells.
Immunofluorescence: a working concentration of
0.5-1 g/mL is recommended using SW-620 cells.
Note: In order to obtain the best results using various
techniques and preparations, we recommend
Product Information Sheet - N8130
indicators: determination of kinetic
parameters by dynamic pH monitoring.
Biotechnol. Bioeng., 72(6), 620-627 (2001).
6. Bazzicalupi, C., et al., CO2 fixation by novel
copper(II) and zinc(II)
Human L-Selectin ELISA Kit
microwell strips are stored at 2 - 8°C
in the dark.
p. Read absorbance of each microwell on a spectrophotometer using 450 nm as the primary
wavelength (optionally 620 nm as the reference wavelength
Acetylcholinesterase (C2888)
Acetylcholinesterase II.
Crystallization, absorption spectra, isoionic point.
Proc. Natl. Acad. Sci. USA, 59(2), 620-623 (1968).
13. Golicnik, M., and Stojan, J., Multi-step analysis as a
tool for kinetic parameter
Data Sheet - SAB4200838
et al., Nat. Cell Biol., 17, 893-906
(2015).
6. Wan, Y., et al., Cell Mol. Immunol., 14, 607-620
(2017).
VS,DR,OKF,MAM 07/19-1
2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
MONOCLONAL ANTI-m-CALPAIN (DOMAIN III) CLONE 9A4H8D3 Mouse Ascites Fluid
calmodulin, or
calpastatin. Epitope mapping studies indicate the
epitope is between amino acids 465-620 (domain III) of
human µ-calpain.
The calpain (Ca2+-dependent proteinase or
Ca2+-activated neutral
Data Sheet - SRP5294
densitometry)
Figure 2.
Specific Activity of Typical Lot:
8–12 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7. 2, 12.5 mM
glycerol 2-phosphate, 25 mM MgC12
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