Showing 1-30 of 30 results for "63630"
Are melibiose-fermenting intestinal and alpechin strains of Saccharomyces cerevisiae a novel type of yeast probiotics?
G I Naumov
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 439, 262-263 (2011-09-29)
Víctor A Lórenz-Fonfría et al.
Methods in molecular biology (Clifton, N.J.), 914, 107-126 (2012-09-15)
The determination of protein conformational changes induced by the interaction of substrates with secondary transporters is an important step toward the elucidation of their transport mechanism. Since conformational changes in a protein alter its vibrational patterns, they can be detected...
[Molecular polymorphism of alpha-galactosidase MEL genes of Saccharomyces yeasts ].
E S Naumova et al.
Mikrobiologiia, 80(4), 496-507 (2011-11-12)
T Pourcher et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 326(1236), 411-423 (1990-01-30)
The role of the co-transported cation in the coupling mechanism of the melibiose permease of Escherichia coli has been investigated by analysing its sugar-binding activity, facilitated diffusion reactions and energy-dependent transport reactions catalysed by the carrier functioning either as an...
G A Eroshenko et al.
Genetika, 47(10), 1328-1334 (2012-01-12)
Comparative analysis of nucleotide sequences of genes participating in melibiose fermentation and isocitrate lyase production was conducted in 90 natural Yersinia pestis strains of main and non main subspecies. It was ascertained that the lack of the ability to utilize...
S Gerber et al.
Chemistry and physics of lipids, 158(2), 118-130 (2009-05-12)
A series of monoacylated glycolipids with even-numbered acyl chain lengths ranging from saturated C11 to C15 and an unsaturated C17:1 fatty acid connected by an amide in linkage to the disaccharide head groups maltose, melibiose and lactose were synthesized. The...
Yibin Lin et al.
Biochimica et biophysica acta, 1828(8), 1690-1699 (2013-03-19)
The melibiose transporter from Escherichia coli (MelB) can use the electrochemical energy of either H(+), Na(+) or Li(+) to transport the disaccharide melibiose to the cell interior. By using spectroscopic and biochemical methods, we have analyzed the role of Arg149...
Mohamed S Elrobh et al.
FEMS microbiology letters, 338(1), 62-67 (2012-10-17)
The Escherichia coli melR gene encodes the MelR transcription factor that controls melibiose utilization. Expression of melR is autoregulated by MelR, which represses the melR promoter by binding to a target that overlaps the transcript start. Here, we show that...
M Cervera-Tison et al.
Applied and environmental microbiology, 78(21), 7720-7732 (2012-08-28)
Ruminococcus gnavus belongs to the 57 most common species present in 90% of individuals. Previously, we identified an α-galactosidase (Aga1) belonging to glycoside hydrolase (GH) family 36 from R. gnavus E1 (M. Aguilera, H. Rakotoarivonina, A. Brutus, T. Giardina, G....
Joana Gasperazzo Ferreira et al.
Applied biochemistry and biotechnology, 164(7), 1111-1125 (2011-02-19)
α-Galactosidases has the potential to hydrolyze α-1-6 linkages in raffinose family oligosaccharides (RFO). Aspergillus terreus cells cultivated on wheat bran produced three extracellular forms of α-galactosidases (E1, E2, and E3). E1 and E2 α-galactosidases presented maximal activities at pH 5...
The melibiose permease of Escherichia coli: importance of the NH2-terminal domains for cation recognition by the Na+/sugar cotransporter.
G Leblanc et al.
Society of General Physiologists series, 48, 213-227 (1993-01-01)
T E Arsen'eva et al.
Zhurnal mikrobiologii, epidemiologii, i immunobiologii, (4)(4), 75-81 (2010-08-28)
To characterize species specificity of officially recommended tests for differentiation of Yersiniapestis and Yersinia pseudotuberculosis and propose additional tests allowing for more accurate identification. Natural, laboratory and typical strains oftwo Yersinia species were studied using microbiological, molecular and biochemical methods....
L Zhang et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(2), 243-248 (2012-05-17)
The gene mel1, encoding alpha-galactosidase in Schizosaccharomyces pombe, and the gene bgl2, encoding and beta-glucosidase in Trichoderma reesei, were isolated and co-expressed in the industrial ethanol-producing strain of Saccharomyces cerevisiae. The resulting strains were able to grow on cellobiose and...
Meritxell Granell et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(51), 22078-22083 (2010-12-08)
The melibiose carrier from Escherichia coli (MelB) couples the accumulation of the disaccharide melibiose to the downhill entry of H(+), Na(+), or Li(+). In this work, substrate-induced FTIR difference spectroscopy was used in combination with fluorescence spectroscopy to quantitatively compare...
Peng Lin et al.
Acta biochimica et biophysica Sinica, 40(12), 1029-1038 (2008-12-18)
A dimeric 50 kDa melibiose-binding lectin was isolated from the seeds of the cultivar of soybean (Glycine max), called the small glossy black soybean. The isolation procedure comprised ion exchange chromatography on Q Sepharose, SP Sepharose and Mono Q followed...
[Versatility in cation coupling and mechanism of transport regulation in the melibiose transport system].
M Kuroda et al.
Seikagaku. The Journal of Japanese Biochemical Society, 66(4), 309-319 (1994-04-01)
Huu-Vang Nguyen et al.
PloS one, 6(10), e25821-e25821 (2011-10-15)
Saccharomyces bayanus is a yeast species described as one of the two parents of the hybrid brewing yeast S. pastorianus. Strains CBS380(T) and NBRC1948 have been retained successively as pure-line representatives of S. bayanus. In the present study, sequence analyses...
Nicole Hugouvieux-Cotte-Pattat et al.
Journal of bacteriology, 191(22), 6960-6967 (2009-09-08)
Erwinia chrysanthemi (Dickeya dadantii) is a plant pathogenic bacterium that has a large capacity to degrade the plant cell wall polysaccharides. The present study reports the metabolic pathways used by E. chrysanthemi to assimilate the oligosaccharides sucrose and raffinose, which...
Lan Guan et al.
The Journal of biological chemistry, 286(8), 6367-6374 (2010-12-15)
The MelB permease of Salmonella typhimurium (MelB-ST) catalyzes the coupled symport of melibiose and Na(+), Li(+), or H(+). In right-side-out membrane vesicles, melibiose efflux is inhibited by an inwardly directed gradient of Na(+) or Li(+) and stimulated by equimolar concentrations...
Ville P Heljo et al.
Journal of pharmaceutical sciences, 102(1), 195-203 (2012-11-15)
Melibiose monohydrate has shown promise when employed as a pharmaceutical excipient, but its physical properties have not been adequately characterized. Therefore, two different melibiose monohydrate batches were analyzed as received or after storage under different relative humidity (RH) atmospheres. The...
Takatsugu Miyazaki et al.
The FEBS journal, 280(18), 4560-4571 (2013-07-06)
Proteins belonging to glycoside hydrolase family 63 (GH63) are found in bacteria, archaea and eukaryotes. Although the eukaryotic GH63 proteins have been identified as processing α-glucosidase I, the substrate specificities of the bacterial and archaeal GH63 proteins are not clear....
[Taxonomy, ecology, and genetics of the yeast Saccharomyces bayanus – new entity in science and practice].
G I Naumov et al.
Mikrobiologiia, 80(6), 723-730 (2012-03-08)
Jennifer L Chaytor et al.
Glycobiology, 22(1), 123-133 (2011-08-20)
The ice recrystallization inhibition activity of various mono- and disaccharides has been correlated with their ability to cryopreserve human cell lines at various concentrations. Cell viabilities after cryopreservation were compared with control experiments where cells were cryopreserved with dimethylsulfoxide (DMSO)....
Lan Guan et al.
Biochemistry, 51(13), 2950-2957 (2012-03-15)
The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or...
S Vivek Jakkula et al.
Journal of bacteriology, 194(20), 5538-5544 (2012-08-07)
The melibiose permease of Salmonella enterica serovar Typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+). Bioinformatics and mutational analyses indicate that a conserved Gly117 (helix IV) is a component of the Na(+)-binding site. In this study, Gly117...
Saswati Chakladar et al.
Biochemistry, 50(20), 4298-4308 (2011-04-19)
The MelA gene from Citrobacter freundii, which encodes a glycosyl hydrolase family 4 (GH4) α-galactosidase, has been cloned and expressed in Escherichia coli. The recombinant enzyme catalyzes the hydrolysis of phenyl α-galactosides via a redox elimination-addition mechanism involving oxidation of...
The melibiose carrier of Escherichia coli.
M C Botfield et al.
Research in microbiology, 141(3), 328-331 (1990-03-01)
Xavier León et al.
Biophysical journal, 96(12), 4877-4886 (2009-06-17)
The melibiose permease (MelB) from Escherichia coli couples the uptake of melibiose to that of Na+, Li+, or H+. In this work, we applied attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy to obtain information about the structural changes...
Hikaru Hemmi et al.
The FEBS journal, 276(7), 2095-2105 (2009-03-19)
The R-type lectin EW29, isolated from the earthworm Lumbricus terrestris, consists of two homologous domains (14,500 Da) showing 27% identity with each other. The C-terminal domain (Ch; C-half) of EW29 (EW29Ch) has two sugar-binding sites in subdomains alpha and gamma...
Ulrike Lingner et al.
Plant physiology, 156(3), 1565-1576 (2011-05-20)
Pathogenic fungi drastically affect plant health and cause significant losses in crop yield and quality. In spite of their impact, little is known about the carbon sources used by these fungi in planta and about the fungal transporters importing sugars...

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