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Meet our experts at World ADC London, March 14-15, 2023!

Meet our experts and learn more about how our services, products and technological solutions can speed up your drug development projects.

Papo com Ciência 4: o futuro sustentável dos negócios

Cannabinoids Analysis Workflow with Chromolith® Column

HPLC-DAD workflow analyzes 14 cannabinoids in hemp extracts in 10 minutes using Chromolith columns for robust performance.

Puriﬁcation or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens

This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.

UHPLC Analysis of Cannabinoids

Cannabis products need cannabinoid characterization. Ascentis Express C18 resolves 14 similar cannabinoids in under seven minutes with excellent peak shape.

UHPLC Analysis Cannabinoids Ascentis Express

Cannabis products need cannabinoid characterization. Ascentis Express C18 resolves 14 similar cannabinoids in under seven minutes with excellent peak shape.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Detergents REACH Information

Explore our comprehensive portfolio of detergents and surfactants as possible Triton X-100 alternatives for your research applications before the REACH Annex 14 ban takes effect.

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Nickel Carbonyl

Analysis of nickel carbonyl, nickel carbonyl in air, organometallics in air, exposure limits for nickel carbonyl, ni(co)4

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Software Simplifies Compliance with 21 CFR Part 11 and EudraLex Good Manufacturing Practice Volume 4 Annex 11

How software can simplify 21 CFR Part 11 and EudraLex Volume 4 Annex 11 compliance and provide additional benefits such as access control, audit trails and easier search and retrieval.

Chromolith® WidePore 300 HPLC Columns

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Heavy Metal Analysis of Cannabis sativa by ICP-MS and the Need for Proper Sample Homogenization

A 4-step comprehensive ICP-MS workflow including sample homogenization, with standard addition calibration method, to determine heavy metals in Cannabis sativa.

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Cell Bio Poster Tube Contest

Are you presenting a poster at Cell Bio December 14-18th, 2024 in San Diego? Carry your poster in style with a MilliporeSigma Poster Tube Carrier. Complete the form below by November 27, 2024 for your chance to win. Contest for

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Removal of Detergents from Biological Product Matrices

The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.

Titripac® Sustainable Packaging for Ready-to-Use Certipur® pH Buffers

Learn about our ready-to-Use Certipur® pH Buffer solutions (4 L & 10 L), available in an innovative Titripac® sustainable packaging for a wide pH range of 1 to 12.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification

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