specifically
phosphorylated on Ser
67
and Ser
457
by Akt, both in vitro
and in vivo. This phosphorylation causes nuclear
translocation of PDCD4.
1
Reagent
Supplied as a solution
plates in growth
media. Cells were cultured for a total of 48 hours, with a final media concentration of 20 µM BrdU added for the final 1 hour of culture. Cell handling, fixation and
immunostaining
A3306 at 2-8 °C.
References
1. Divakaran, D. et al., Braz. J. Microbiol., 42(4),
1397-1404 (2011).
2. Medda, S., and Chandra, A., J. Appl. Bacteriol.,
48(1), 47-58 (1980).
3. Morgan
8850 1 pac $45
TE Buffer Solution 8890 100 ml $43
OmniPur® 100X TE Buffer Solution 8910 1 l $152
OmniPur® HEPES 5310
5320
5330
5340
5360
100 g
500 g
1 kg
5
coefficient:48 EmM = 100 (403 nm)
Optimal pH range:49 6.0-6.5 (activity at pH 7.5 is
84% of the maximum)
The enzyme is most stable in the pH range of 5.0-9.0.
Isoelectric point:1
8°C in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date
substance
Synonyms 3-(1-Methyl-2-pyrrolidinyl)pyridine
Stability If stored at 2-8°C in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening
tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date December 2008
Storage
in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date
8°C in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date
1.0 EU per 1 µg cytokine as
determined by the LAL (Limulus amebocyte lysate)
method.
References
1. Stempien, M., et al., DNA, 5, 357 (1986).
2. Karey, K.P., et al., Cancer Research, 48, 4083
in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date
proteins.
Anti-Munc-18-1 recognizes specifically Munc-18-1 (67
kDa). Applications include the detection of Munc-18-1
by immunoblotting and immunofluorescence. Staining of
the Munc-18-1 band in immunoblotting
2-8°C in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date September
pentyl-
6H-dibenzo[b ,d]pyran-1-ol; CBN
Stability If stored at 2-8°C in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration
8°C in an air tight container,
protected from light, decomposition of the
product is less than 1% in 48 months. After
opening, concentration may change due to
loss of solvent.
Expiration Date
thawing is not
recommended.
References
1. Vogt, W. et al., Immunology, 34(1), 29-40 (1978).
2. Gigli, I., and Nelson, R.A. Jr., Exp. Cell. Res.,
51(1), 45-67 (1968).
3. Mueller-Eberhard, H.J. et al