Journal of the American Chemical Society, 134(28), 11525-11532 (2012-06-14)
A method was developed to detect fluorescence intensity signals from single molecules diffusing freely in a capillary cell. A unique optical system based on a spherical mirror was designed to enable quantitative detection of the fluorescence intensity. Furthermore, "flow-and-stop" control...
Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy.
Zhang, P.; Goodwin, P.M.; Werner, J.H.
Optics Express, 22(10), 12398-12409 (2014)
Technical Review. Types of Imaging-Direct STORM.
Jensen, E.; Crossman, D. J.
The Anatomical Record, 297(12), 2227-2231 (2014)
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig K.I.; et al.
Nature, 440(7086), 935-939 (2006)
Sub-diffraction imaging of nitrogen-vacancy centers in diamond by stimulated emission depletion and structured illumination
Xusan, Y., et al.
Royal Society of Chemistry Advances, 4(22), 11305-11305 (2014)
Chemistry (Weinheim an der Bergstrasse, Germany), 16(1), 158-166 (2009-12-02)
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been...
We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require...
Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay.
Lin, P.Y.; et al
Optics Express, 20(10), 11445-11450 (2012)
Block Copolymer Nanostructures Mapped by Far-Field Optics.
Ullal, C.K.; et al
Nano Letters, 9(6), 2497-2500 (2009)
Frequency dependent detection in a STED microscope using modulated excitation light.
Ronzitti, E.; Harke, B.; Diaspro, A.
Optics Express, 21(1), 210-210 (2013)
Maturation of active zone assembly by Drosophila Bruchpilot.
Fouquet, W.; et al.
The Journal of Cell Biology, 186(1), 129-145 (2009)
STED microscopy resolves nanoparticle assemblies.
Willig K.I.; et al.
New Journal of Physics, 8(6), 106-106 (2006)
Spatial organization of proteins in metastasizing cells.
Ronnlund, D.; et al.
Cytometry, 83(9), 855-865 (2013)
2PE-STED Microscopy with a Single Ti. Sapphire Laser for Reduced Illumination.
The endoplasmic reticulum is the site of folding, assembly and quality control for proteins of the secretory pathway. The ATP-regulated Hsp70 chaperone BiP (heavy chain-binding protein), together with cochaperones, has important roles in all of these processes. The functional cycle...
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B.; et al
Optical Nanoscopy, 1(1), 3-3 (2012)
STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers.
Laporte, G.; Psaltis, D.
Biomedical Optics Express, 7(1), 34-44 (2016)
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)
Experimental Proof of Concept of Nanoparticle-Assisted STED.
Sonnefraud, Y.; et al
Nano Letters, 14(8), 4449-4453 (2014)
Nanoscale organization of nicotinic aceylcholine receptors by stimulated emission depletion microscopy.
Kellner, R.R., et al.
Neuroscience, 144(1), 135-143 (2007)
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Hofmann, M.; et al.
Proceedings of the National Academy of Sciences of the USA, 102(49), 17565?17569-17565?17569 (2005)
Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.
Stimulated emission depletion (STED) microscopy usually employs a scanning excitation beam that is superimposed by a donut-shaped STED beam for keeping the fluorophores at the periphery of the excitation spot dark. Here, we introduce a simple birefringent device that produces...
The GroEL/GroES chaperonin system mediates protein folding in the bacterial cytosol. Newly synthesized proteins reach GroEL via transfer from upstream chaperones such as DnaK/DnaJ (Hsp70). Here we employed single molecule and ensemble FRET to monitor the conformational transitions of a...
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.
The self-assembly properties of DNA make it an ideal choice for the construction of nanomachines such as DNA tweezers. Because nanomachines function as individual units, they need to be studied on the single-molecule level. From single-pair FRET investigations, we show...
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 510-517 (2011-02-03)
H(+)-ATP synthases are molecular machines which couple transmembrane proton transport with ATP synthesis from ADP and inorganic phosphate by a rotational mechanism. Single-pair fluorescence resonance energy transfer (spFRET) in single molecules is a powerful tool to analyse conformational changes. It...
Proceedings of the National Academy of Sciences of the United States of America, 103(31), 11440-11445 (2006-07-26)
We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has been enabled by the elimination of molecular triplet state excitation as a...
Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.
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