Hybridization of nucleic acids with secondary structure is involved in many biological processes and technological applications. To gain more insight into its mechanism, we have investigated the kinetics of DNA hybridization/denaturation via fluorescence resonance energy transfer (FRET) on perfectly matched...
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit...
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope.
Meyer, S. A.; Ozbay, B.
Proceedings of SPIE, 8950, 89500-89500 (2014)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
Single-particle and ensemble diffusivities--test of ergodicity.
Florian Feil et al.
Angewandte Chemie (International ed. in English), 51(5), 1152-1155 (2011-10-19)
Chemistry (Weinheim an der Bergstrasse, Germany), 18(41), 12986-12998 (2012-09-13)
Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the...
SERRS-based detection of dye-labeled DNA using positively-charged Ag nanoparticles.
Gill, R. and Lucassen, G. W.
Analytical Methods : Advancing Methods and Applications, 2, 445-447 (2010)
We report the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving the fluorophore in a metastable dark state. Using a relatively modest light intensity of several kW/cm(2) in a focal distribution featuring a local zero...
Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier.
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100...
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.
Fitzpatrick, JA.; et al.
Bioconjugate Chemistry, 20(10), 1843-1847 (2009)
Quantification of Internalized Silica Nanoparticles via STED Microscopy.
Peuschel, H.; et al.
BioMed Research International, 2015(1), 1-16 (2015)
An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
Vicidomini, G.; et al.
Nano Letters, 15(9), 5912-5918 (2015)
Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye.
Luo, D., et al.
Optics & Laser technology, 45, 723-725 (2013)
Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells.
Stagge, F.; et al.
PLoS ONE, 8(10), e78745-e78745 (2013)
Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95.
Nair D.; et al.
The Journal of Neuroscience, 33(32), 13204-13224 (2013)
We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in...
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