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6B100

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P Upcroft et al.
Journal of chromatography, 618(1-2), 79-93 (1993-08-25)
Agarose as a medium for separation of DNA was first introduced in 1962 and since the early 1970s agarose submarine gel electrophoresis has been synonymous with separations of DNA molecules larger than 1 kilobase pair (kb). The large pore size...
Erlinda Fernández et al.
PloS one, 8(2), e56417-e56417 (2013-03-06)
Recombinant virus-like particles (VLP) antigenically similar to rabbit hemorrhagic disease virus (RHDV) were recently expressed at high levels inside Pichia pastoris cells. Based on the potential of RHDV VLP as platform for diverse vaccination purposes we undertook the design, development...
N C Stellwagen et al.
Electrophoresis, 14(4), 355-368 (1993-04-01)
The orientation of the agarose gel matrix in pulsed electric fields has been studied by transient electric birefringence. Two types of agarose with different degrees of charge were studied, in addition to agarose solutions and gels containing beta-carrageenan, a stereoisomer...
Pierre Martine et al.
Cell death & disease, 10(4), 256-256 (2019-03-16)
The NOD-leucine rich repeat and pyrin containing protein 3 (NLRP3) inflammasome is a multi-protein complex, aimed at producing IL-1β in response to danger signals which must be tightly regulated. Here we investigated the importance of the stress sensor, Heat Shock...
Avital Mendelson et al.
Lab on a chip, 13(6), 1156-1164 (2013-02-01)
A multitude of cytotactic cues direct cell migration in development, cancer metastasis and wound healing. However, our understanding of cell motility remains fragmented partially because current migration devices only allow the study of independent factors. We developed a cell motility...
J C Wallwork et al.
The Journal of biological chemistry, 252(17), 5977-5980 (1977-09-10)
Histone-lysine methyltransferase has been solubilized from rat brain chromatin by repeated extraction with distilled water. The enzyme was further purified by chromatography on DEAE-cellulose and gel filtration. With chromosomal-bound histones as substrates, the enzyme methylated only the lysyl residues in...
Pablo Navarro et al.
Human genetics, 130(2), 255-264 (2011-05-06)
X-inactivation, the molecular mechanism enabling dosage compensation in mammals, is tightly controlled during mouse early embryogenesis. In the morula, X-inactivation is imprinted with exclusive silencing of the paternally inherited X-chromosome. In contrast, in the post-implantation epiblast, X-inactivation affects randomly either...
H J Lubberding et al.
European journal of biochemistry, 137(1-2), 95-99 (1983-12-01)
The ATPase complex is isolated and purified from membrane vesicles of the thermophilic cyanobacterium Synechococcus 6716 by octyl glucoside and cholic acid by a modification of the procedure for its extraction from spinach chloroplasts. The complex is purified by differential...
Jia Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(17), 6694-6699 (2013-04-10)
Seamless and minimally invasive integration of 3D electronic circuitry within host materials could enable the development of materials systems that are self-monitoring and allow for communication with external environments. Here, we report a general strategy for preparing ordered 3D interconnected...
Haruka Koga et al.
Biofabrication, 5(1), 015010-015010 (2013-01-11)
Micropatterning is useful for regulating culture environments. We developed a highly efficient near-infrared-(NIR)-responsive gel and established a new technique that enables cell patterning by NIR irradiation. As a new culture substratum, we designed a tissue culture plate that was coated...
F H Kirkpatrick et al.
Electrophoresis, 14(4), 349-354 (1993-04-01)
Properties of agarose potentially relevant to PFGE (pulsed-field gel electrophoresis) are reviewed, and some new information is presented. Agarose polymers appear to have molecular weights in the range of 100,000 to 200,000 Da, but this is not tightly related to...
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 74(6), 16-23 (2013-01-09)
A scheme of isolation and purification of the enzyme with alpha-L-rhamnosidase activity from culture liquid Cryptococcus albidus 1001 has been developed. It included fractionation by ammonium sulfate and chromatography on TSK-gels Toyopearl HW-60, Fractogel TSK DEAE-650-s and Sepharose 6B. The...
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized...
Minglin Sun et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 27(1), 89-94 (2013-02-23)
To explore the effect of rolling compression loading bioreactor on chondrogenesis of rabbit bone marrow mesenchymal stem cells (BMSCs) with different loading parameters. BMSCs were isolated from New Zealand rabbits, aged 2.5 months. BMSCs at passage 3 were used to...
H N Dao et al.
Analytical biochemistry, 196(1), 46-53 (1991-07-01)
A method for producing lipid vesicles containing fluorescent phospholipid analogues localized to the inner leaflet of their membrane was developed. Incubation of a 450-fold molar excess of serum albumin with lipid vesicles symmetrically labeled with 1 mol % 1-palmitoyl-2-(N-4-nitrobenzo-2-oxa-1,3-diazolyl)amino-caproyl phosphatidylcholine...
Immunochemical studies on chlamydial group antigen (presence of a 2-keto-3-deoxycarbohydrate as immunodominant group).
S P Dhir et al.
Journal of immunology (Baltimore, Md. : 1950), 109(1), 116-122 (1972-07-01)
Wen-Jun Han et al.
The Journal of biological chemistry, 288(13), 9519-9531 (2013-02-05)
Agarase hydrolyzes agarose into a series of oligosaccharides with repeating disaccharide units. The glycoside hydrolase (GH) module of agarase is known to be responsible for its catalytic activity. However, variations in the composition of the GH module and its effects...
Ming Sin Cheung et al.
Journal of immunology (Baltimore, Md. : 1950), 198(5), 2063-2069 (2017-02-01)
Periodic fever with aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) is a relatively common autoinflammatory condition that primarily affects children. Although tendencies were reported for this syndrome, genetic variations influencing risk and disease progression are poorly understood. In this study...
Tobias K H Müller et al.
Journal of chromatography. A, 1285, 97-109 (2013-03-14)
An integrated approach to temperature-controlled chromatography, involving copolymer modified agarose adsorbents and a novel travelling cooling zone reactor (TCZR) arrangement, is described. Sepharose CL6B was transformed into a thermoresponsive cation exchange adsorbent (thermoCEX) in four synthetic steps: (i) epichlorohydrin activation;...
Sivakumar Vallabhapurapu et al.
Nature immunology, 9(12), 1364-1370 (2008-11-11)
The adaptor and signaling proteins TRAF2, TRAF3, cIAP1 and cIAP2 may inhibit alternative nuclear factor-kappaB (NF-kappaB) signaling in resting cells by targeting NF-kappaB-inducing kinase (NIK) for ubiquitin-dependent degradation, thus preventing processing of the NF-kappaB2 precursor protein p100 to release p52....
Tatsuya Kawai et al.
Acta radiologica (Stockholm, Sweden : 1987), 54(8), 954-960 (2013-04-25)
The effects of a tin filter on virtual non-enhanced (VNE) images created by dual-energy CT have not been well evaluated. To compare the accuracy of VNE images between those with and without a tin filter. Two different types of columnar...
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and...
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies....
Joshua Schindel et al.
International journal of radiation oncology, biology, physics, 86(2), 387-393 (2013-02-26)
To present a novel marker-flange, addressing source-reconstruction uncertainties due to the artifacts of a titanium intracavitary applicator used for magnetic resonance imaging (MRI)-guided high-dose-rate (HDR) brachytherapy (BT); and to evaluate 7 different MRI marker agents used for interstitial prostate BT...
Hu Liu et al.
Journal of chromatography. A, 1344, 59-65 (2014-04-29)
This work proposed an integrative method of protein refolding and purification by like-charged resin facilitated refolding and metal-chelate affinity adsorption. Hexahistidine-tagged enhanced green fluorescence protein (EGFP) was overexpressed in Escherichia coli as inclusion bodies (IBs), and then the protein was...
Lan Zhao et al.
Biomedical chromatography : BMC, 27(9), 1089-1091 (2013-05-18)
To study the leakage at different solution pH values, IgG Sepharose 6FF®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which...
J A Duerre et al.
Biochimica et biophysica acta, 843(1-2), 58-67 (1985-11-22)
The histone lysine methyltransferases catalyze the transfer of methyl groups from S-adenosylmethionine to specific epsilon-N-lysine residues in the N-terminal regions of histones H3 and H4. These enzymes are located exclusively within the nucleus and are firmly bound to chromatin. The...
Gianluca Vadalà et al.
Spine, 38(6), E319-E324 (2013-01-18)
Descriptive anatomical study on ovine and human cadaveric lumbar spinal segments. To describe the alternative transpedicular approach to deliver therapeutic agents into intervertebral disc (IVD). The present delivery approach of therapeutic agents (growth factors/cells/hydrogels) within the IVD is through injection...
Purification of recombinant protein derived from the baculovirus expression system using glutathione affinity agarose.
E J Sorscher et al.
Methods in molecular biology (Clifton, N.J.), 39, 337-348 (1995-01-01)
Takayuki Takei et al.
Journal of bioscience and bioengineering, 117(4), 497-500 (2013-11-05)
Agarose gels were superior to calcium-alginate gels for immobilizing Rhodococcus erythropolis CS98 strain to remove cesium from water. Suitable incubation time of the immobilized cells in cesium solutions, cell number in the gels and volume ratio of the cesium solution...
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