Showing 1-30 of 57 results for "70215"
Marcos Mandaji et al.
Electrophoresis, 30(9), 1510-1515 (2009-04-08)
In a previous work [M. Mandaji, et al., this issue] a sample stacking method was theoretically modeled and experimentally demonstrated for analytes with low dpK(a)/dT (analytes carrying carboxylic groups) and BGEs with high dpH/dT (high pH-temperature-coefficients). In that work, buffer...
Maria Notou et al.
Journal of chromatography. A, 1356, 272-276 (2014-07-06)
A novel zone-fluidics/high pressure liquid chromatographic (ZF-HPLC) method is described for the simultaneous determination of six biogenic monoamines, in the presence of hexylamine as internal standard. Automated on-line derivatization of the analytes with naphthalene-2,3-dicarboxaldehyde/cyanide ions was performed using the ZF...
Qiang Chu et al.
Journal of materials chemistry. B, 8(37), 8546-8557 (2020-08-26)
Amino acids are the fundamental building blocks of proteins in tumor cells. The consumption of amino acid can be an effective approach for destroying the tumor cytoskeleton and malfunctioning of the intracellular metabolic balance. Following this concept, herein, amino acid...
An improved mechanism-based cross-linker for multiplexed kinase detection and inhibition in a complex proteome.
Kai Liu et al.
Chembiochem : a European journal of chemical biology, 9(12), 1883-1888 (2008-07-16)
Xue Wang et al.
Analytical chemistry, 88(6), 3369-3375 (2016-02-19)
Islets of Langerhans are the regulators of in vivo blood glucose levels through the secretion of endocrine hormones. Amino acids, released from various cells within islets or from intrapancreatic neurons, are hypothesized to further adjust hormone secretions. In contrast to...
Takashi Kaneta et al.
Electrophoresis, 32(9), 1061-1067 (2011-03-31)
Previously, we have demonstrated postcolumn derivatization of proteins separated by capillary sieving electrophoresis (CSE), in which naphthalene-2,3-dicarbaldehyde was employed as a fluorogenic labeling reagent. Standard proteins separated by CSE were reacted with naphthalene-2,3-dicarbaldehyde in the presence of 2-mercaptoethanol (2-ME) which...
Ncediwe Ndube et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(23), 2239-2243 (2011-07-06)
Fumonisins are mycotoxins produced by various species of Fusarium and occur naturally in contaminated maize and maize-based foods. Ingestion of fumonisins has considerable health implications for humans and animals. Since fumonisins lack a useful chromophore or fluorophore, their determination in...
P. de Montigny et al.
Analytical Chemistry, 59, 1096-1096 (1987)
Ilaria Del Gaudio et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865(4), 158632-158632 (2020-01-19)
Perinatal and long-term offspring morbidities are strongly dependent on the preservation of placental vascular homeostasis during pregnancy. In adults, the HDL-apoM-S1P complex protects the endothelium and maintains vascular integrity. However, the metabolism and biology of cord blood-derived HDLs (referred to...
Yoshifumi Hagiya et al.
Toxicology and applied pharmacology, 282(2), 195-206 (2014-12-17)
The key mechanism for acetaminophen hepatotoxicity is cytochrome P450 (CYP)-dependent formation of N-acetyl-p-benzoquinone imine, a potent electrophile that forms protein adducts. Previous studies revealed the fundamental role of glutathione, which binds to and detoxifies N-acetyl-p-benzoquinone imine. Glutathione is synthesized from...
Anastasia Zotou et al.
Analytical and bioanalytical chemistry, 403(4), 1039-1048 (2011-11-22)
A new fluorescence-HPLC method was developed for the simultaneous determination of eight biogenic monoamines (histamine, methylamine, tyramine, ethylamine, propylamine, tryptamine, 2-phenylethylamine, isoamylamine) and two biogenic diamines (putrescine, cadaverine) in the presence of heptylamine as the internal standard. The amines were...
Takashi Kaneta et al.
Electrophoresis, 30(21), 3780-3785 (2009-10-29)
The separation methods for proteins with high resolution and sensitivity are absolutely important in the field of biological sciences. Capillary sieving electrophoresis (CSE) is an excellent separation technique for DNA and proteins with high resolution, while LIF permits the most...
Eric W Crick et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 57, 25-33 (2014-01-28)
The goal of this study was to use a status epilepticus steady-state chemical model in rats using the convulsant, 3-mercaptopropionic acid (3-MPA), and to compare the changes in striatal neurotransmission on a slow (5min) and fast (60s) timescale. In vivo...
En-Ping Lin et al.
Talanta, 114, 297-303 (2013-08-21)
This study proposes a sensitive method for the simultaneous separation and concentration of 9 pairs of amino acid enantiomers by combining poly(ethylene oxide) (PEO)-based stacking, β-cyclodextrin (β-CD)-mediated micellar electrokinetic chromatography (MEKC), and 9-fluoroenylmethyl chloroformate (FMOC) derivatization. The 9 pairs of...
Dongguang Yin et al.
Luminescence : the journal of biological and chemical luminescence, 23(6), 434-438 (2008-09-19)
In the present study, a novel peroxyoxalate CE-CL system was developed to achieve high signal stability and sensitivity based on a design of a new interface including a new mixing mode and a new grounding electrode mode. Amino acids fluorescently...
S.M. Lunte et al.
LC-GC, 7, 908-908 (1989)
Ming-Mu Hsieh et al.
Methods in molecular biology (Clifton, N.J.), 984, 121-129 (2013-02-07)
In this chapter, we describe a method to identify amino acids (AA) by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF). First, amino acids labeled with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide are converted to highly fluorescent cyanobenz[f]isoindole...
Saionara Sartor et al.
Journal of food science, 84(12), 3546-3554 (2019-11-12)
Sparkling wines were elaborated with the nontraditional varieties Villenave, Niagara, Manzoni, and Goethe, and monitored in relation to the changes in phenolic composition, browning index, and glutathione content during 18 months of biological aging (sur lies). Important changes in the...
Yoann Ladner et al.
Electrophoresis, 33(19-20), 3087-3094 (2012-09-25)
This article shows that there is great interest in using an electrochromatographic microchip made of hexyl acrylate (HA) based porous monolith cast within the channel of a cyclic olefin copolymer (COC) device. The monolith is simultaneously in situ synthesized and...
Yu Hui et al.
Electrophoresis, 33(12), 1911-1920 (2012-06-29)
Asymmetric dimethylarginine (ADMA) is an inhibitor of nitric oxide synthase and a risk factor for cardiovascular events. We have developed a new derivatization method to enable baseline separation of the regio-isomers, ADMA, and symmetric dimethylarginine (SDMA), within 15 min on...
Wei Chen et al.
Chemico-biological interactions, 219, 83-89 (2014-06-01)
Acrylamide (AA), a well-known toxicant, has attracted numerous attentions for its presumably carcinogenesis, neurotoxicity and cytotoxicity. Oxidative stress was considered to be associated with acrylamide cytotoxicity, but the link between oxidative stress and acrylamide cytotoxicity is still unclear. In the...
Gail M Johnson et al.
Free radical biology & medicine, 63, 476-484 (2013-05-21)
Donors of nitroxyl (HNO) have shown promise for treatment of stroke, heart failure, alcoholism and cancer. However, comparing the pharmacological capacities of various donors is difficult without first quantifying the amount of HNO released from each donor. Detection and quantitation...
Adeniyi A Adenuga et al.
Chemosphere, 147, 396-403 (2016-01-18)
Diesel exhaust particles (DEP) are a major constituent of ambient air pollution and are associated with various adverse health effects, posing a major safety and public health concern in ambient and occupational environments. The effects of DEP from various biodiesel...
Hyesung Lee et al.
Nutrition research and practice, 4(1), 23-29 (2010-03-04)
Poly-gamma-glutamic acid (gamma-PGA) is a mucilaginous and biodegradable compound produced by Bacillus subtilis from fermented soybeans, and is found in the traditional Korean soy product, cheongkukjang. This study was carried out to evaluate the effects of gamma-PGA from a food...
Sandrine Parrot et al.
ACS chemical neuroscience, 8(9), 1839-1846 (2017-06-16)
Histamine has been shown to modulate visual system and photic behavior in arthropods. However, few methods are available for the direct quantification of histamine and its precursor and metabolites in arthropod brain. In this work, a method for the separation...
Dokyong Kim et al.
Biochemistry, 57(35), 5210-5217 (2018-03-29)
In the Suf Fe-S cluster assembly pathway, the activity of the cysteine desulfurase, SufS, is regulated by interactions with the accessory sulfotransferase protein, SufE. SufE has been shown to stimulate SufS activity, likely by inducing conformational changes in the SufS...
Audrey Rodat-Boutonnet et al.
Electrophoresis, 33(12), 1709-1714 (2012-06-29)
LEDs present an alternative to lasers in LIF detection with CE, resulting in LED-induced fluorescence (LEDIF). LEDs are much less expensive, consume less energy and are more stable. In addition, LED light sources allow a greater range of wavelengths to...
Yuting Li et al.
Food chemistry, 243, 65-73 (2017-11-18)
Increasing evidence indicates that crude extracts derived from mulberry confer protection against oxidative stress. However, the antioxidant capacity of mulberry extract among different cultivars remains elusive. The main objective of the present study was therefore to investigate the effect of...
Li-Yao Zhang et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877(31), 4051-4054 (2009-10-28)
A rapid analytical method was developed for the quantitative determination of glutathione (GSH) using capillary electrophoresis and fluorescence detection. A fluorescence derivatization reagent, naphthalene-2,3-dicarboxaldehyde (NDA) was successfully applied to label GSH. The optimal derivatization reaction was performed with 5.0 mM...

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