Showing 1-30 of 1586 results for "

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Carina Lindemann et al.
Acta neuropathologica, 122(2), 241-251 (2011-05-19)
The suppressor of cytokine signaling 3 (SOCS3) gene is one of eight structurally related genes of the SOCS family and has been suggested to function as a tumor suppressor by inhibition of the JAK/STAT signaling pathway. We investigated 60 human...
Victoria A Pudney et al.
European journal of immunology, 40(3), 899-910 (2009-12-30)
Stimulation of high-avidity CTL responses is essential for effective anti-tumor and anti-viral vaccines. In this study we have demonstrated that a DNA vaccine incorporating CTL epitopes within an Ab molecule results in high-avidity T-cell responses to both foreign and self...
Qian Shu et al.
PLoS pathogens, 11(9), e1005150-e1005150 (2015-09-16)
Interferon stimulated genes (ISGs) target viruses at various stages of their infectious life cycles, including at the earliest stage of viral entry. Here we identify ArfGAP with dual pleckstrin homology (PH) domains 2 (ADAP2) as a gene upregulated by type...
A kindred with mutant IKAROS and autoimmunity.
Erika Van Nieuwenhove et al.
The Journal of allergy and clinical immunology, 142(2), 699-702 (2018-05-01)
Tidi Hassan et al.
Nucleic acids research, 41(6), e71-e71 (2013-01-18)
MicroRNAs (miRNAs) are small non-coding RNAs that regulate expression by translational repression or messenger RNA (mRNA) degradation. Although numerous bioinformatic prediction models exist to identify miRNA-mRNA interactions, experimental validation of bona fide interactions can be difficult and laborious. Few methods...
Meredith E Stewart et al.
FEBS open bio, 5, 138-146 (2015-04-04)
Bluetongue virus (BTV) encodes a single capping protein, VP4, which catalyzes all reactions required to generate cap1 structures on nascent viral transcripts. Further, structural analysis by X-ray crystallography indicated each catalytic reaction is arranged as a discrete domain, including a...
Samuel A Curran et al.
PloS one, 9(5), e98627-e98627 (2014-05-31)
The incidence of atherosclerosis is significantly increased in rheumatoid arthritis (RA). Infection is one factor that may be involved in the pathogenesis of both diseases. The cause of RA and atherosclerosis is unknown, and infection is one of the factors...
Laura Brohée et al.
Oncotarget, 6(13), 11264-11280 (2015-04-03)
Lipogenesis inhibition was reported to induce apoptosis and repress proliferation of cancer cells while barely affecting normal cells. Lipins exhibit dual function as enzymes catalyzing the dephosphorylation of phosphatidic acid to diacylglycerol and as co-transcriptional regulators. Thus, they are able...
Azadeh Arabi et al.
Nature communications, 3, 976-976 (2012-08-07)
Fbw7 is a ubiquitin-ligase that targets several oncoproteins for proteolysis, but the full range of Fbw7 substrates is not known. Here we show that by performing quantitative proteomics combined with degron motif searches, we effectively screened for a more complete...
Sergio E Alvarez et al.
Nature, 465(7301), 1084-1088 (2010-06-26)
Tumour-necrosis factor (TNF) receptor-associated factor 2 (TRAF2) is a key component in NF-kappaB signalling triggered by TNF-alpha. Genetic evidence indicates that TRAF2 is necessary for the polyubiquitination of receptor interacting protein 1 (RIP1) that then serves as a platform for...
Clíona Ní Cheallaigh et al.
Immunity, 44(2), 368-379 (2016-02-18)
Humans that are heterozygous for the common S180L polymorphism in the Toll-like receptor (TLR) adaptor Mal (encoded by TIRAP) are protected from a number of infectious diseases, including tuberculosis (TB), whereas those homozygous for the allele are at increased risk....
Julia I Ellyard et al.
European journal of immunology, 41(2), 485-490 (2011-01-27)
Activation-induced deaminase (AID) is a B lymphocyte-specific DNA deaminase that triggers Ig class-switch recombination (CSR) and somatic hypermutation. It shuttles between cytoplasm and nucleus, containing a nuclear export sequence (NES) at its carboxyterminus. Intriguingly, the precise nature of this NES...
Kelly K Hunt et al.
Breast cancer research : BCR, 15(1), R3-R3 (2013-01-17)
Elafin is an elastase-specific inhibitor with increased transcription in normal mammary epithelial cells compared to mammary carcinoma cells. In this report, we test the hypothesis that inhibition of elastase, through induction of elafin, leads to inhibition of human breast cancer...
James H Felce et al.
Cell reports, 20(11), 2654-2665 (2017-09-14)
The organization of Rhodopsin-family G protein-coupled receptors (GPCRs) at the cell surface is controversial. Support both for and against the existence of dimers has been obtained in studies of mostly individual receptors. Here, we use a large-scale comparative study to...
Hsiao-Ping Lee et al.
International orthopaedics, 38(9), 1923-1930 (2014-06-26)
Our aim was to evaluate whether nonviral vectors can genetically modify primary human juvenile and adult meniscal fibrochondrocytes at low toxicity in vitro and to test the hypothesis that transfected human meniscal fibrochondrocytes transplanted into longitudinal defects and onto human...
Joseph D Comber et al.
Journal of virology, 85(13), 6453-6463 (2011-04-29)
Major histocompatibility complex (MHC) class II-presented peptides can be derived from both exogenous (extracellular) and endogenous (biosynthesized) sources of antigen. Although several endogenous antigen-processing pathways have been reported, little is known about their relative contributions to global CD4(+) T cell...
Kayo Yamada et al.
Molecular cell, 49(5), 922-933 (2013-02-05)
The ARF tumor suppressor is a central component of the cellular defense against oncogene activation in mammals. p14ARF activates p53 by binding and inhibiting HDM2, resulting, inter alia, in increased transcription and expression of the cyclin-dependent kinase inhibitor p21 and...
Hironori Kawahara et al.
The Journal of biological chemistry, 286(18), 16121-16130 (2011-03-08)
Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem/progenitor cells (NS/PCs) as well as in other tissue stem cells. Msi1 binds to the 3'-UTR of its target mRNAs in NS/PCs, prevents their translation, and interferes with...
J Turpin et al.
Oncogene, 35(47), 6053-6064 (2016-05-10)
Amplification and overexpression of erbB2/neu proto-oncogene is observed in 20-30% human breast cancer and is inversely correlated with the survival of the patient. Despite this, somatic activating mutations within erbB2 in human breast cancers are rare. However, we have previously...
Hong-Sheng Zhang et al.
FEBS letters, 589(24 Pt B), 4106-4111 (2015-12-04)
EZH2 plays a major role in HIV-1 latency, however, the molecular linkage between Tat-induced HIV-1 transactivation and EZH2 activity is not fully understood. It was shown Tat induced HIV-1 transactivation through inhibiting EZH2 activity. Tat decreased the levels of H3K27me3...
Alexandra Stolz et al.
The EMBO journal, 36(4), 549-564 (2016-12-29)
Autophagy is a cellular surveillance pathway that balances metabolic and energy resources and transports specific cargos, including damaged mitochondria, other broken organelles, or pathogens for degradation to the lysosome. Central components of autophagosomal biogenesis are six members of the LC3...
Florentina Rus et al.
Methods in molecular biology (Clifton, N.J.), 890, 273-288 (2012-06-13)
Innate immune recognition of pathogens is critical to the prompt control of infections, permitting the host to survive to develop long-term immunity via an adaptive immune response. Poxviruses encode a family of proteins that inhibit signaling by Toll-like receptors to...
Zakaria Grada et al.
Molecular therapy. Nucleic acids, 2, e105-e105 (2013-07-11)
Targeted T cells are emerging as effective non-toxic therapies for cancer. Multiple elements, however, contribute to the overall pathogenesis of cancer through both distinct and redundant mechanisms. Hence, targeting multiple cancer-specific markers simultaneously could result in better therapeutic efficacy. We...
Kendi Okuda et al.
PLoS pathogens, 12(6), e1005669-e1005669 (2016-06-10)
Leishmania amastigotes manipulate the activity of macrophages to favor their own success. However, very little is known about the role of innate recognition and signaling triggered by amastigotes in this host-parasite interaction. In this work we developed a new infection...
S Vimercati et al.
Toxicology in vitro : an international journal published in association with BIBRA, 60, 116-124 (2019-05-21)
Cytochrome P450 enzymes (CYPs) of the equine CYP3A subfamily are predominantly involved in drug metabolism. In this study, genetic variants of the equine CYP3A94, CYP3A95, and CYP3A97 were identified and characterized using in silico modeling and in vitro enzyme kinetics....
Atsushi Shibata et al.
Molecular cell, 53(1), 7-18 (2013-12-10)
MRE11 within the MRE11-RAD50-NBS1 (MRN) complex acts in DNA double-strand break repair (DSBR), detection, and signaling; yet, how its endo- and exonuclease activities regulate DSBR by nonhomologous end-joining (NHEJ) versus homologous recombination (HR) remains enigmatic. Here, we employed structure-based design...
Giovanna Nicolaou et al.
Journal of atherosclerosis and thrombosis, 19(2), 137-148 (2011-11-30)
Atherosclerotic lesions contain DNA signatures from a wide variety of bacteria, although little is known of how exposure to these organisms may modulate the accumulation of lipids in macrophages. To address this, a panel of nine bacteria representing those most...
Bodil Salvesen et al.
Molecular immunology, 47(6), 1226-1234 (2010-01-23)
Meconium, the first intestinal discharge of the newborn, contains material accumulated during fetal life. Meconium activates complement and CD14 and may induce a systemic inflammatory response. Toll-like receptors are classical pattern-recognition receptors recognizing both exogenous and host-derived ligands. The cyanobacterial...
Kasandra Bélanger et al.
Virology, 448, 168-175 (2013-12-10)
Enzymatic deamination of cytidines in DNA is an intrinsic component of antibody maturation and retroviral resistance, but can also be a source of HIV drug resistance and cancer-causing mutations. Here, we developed a high-throughput method based on high resolution melt...
Aleksandra A Watson et al.
The Journal of biological chemistry, 286(27), 24208-24218 (2011-05-14)
The human C-type lectin-like molecule CLEC5A is a critical macrophage receptor for dengue virus. The binding of dengue virus to CLEC5A triggers signaling through the associated adapter molecule DAP12, stimulating proinflammatory cytokine release. We have crystallized an informative ensemble of...