Phosphatase for 2 minutes at +75°C.
Use the dephosphorylation mixture directly for the ligation reaction or store the mixture at −15 to −25°C.
1
2
3
4
5
12
sigma-aldrich.com 7
3. Additional Information on this Product
3.1. Test Principle
Principle of the standard test
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04
21
.0
37
31
87
PASSAGING CELLS:
Passage of the C57/BL6 ES cells from a 25 cm2 flask to a 75 cm2 flask.
1. Prepare a 75 cm2 flask with 0.1% Gelatin Solution.
2. Examine
version: March 2017
Set of 75 short hydrolysis probes for gene expression quantification of multiple organism levels by
real-time PCR
Cat. No. 04 869 877 001 1 set
75 Universal ProbeLibrary probes
binding to the resin.
1 3 5 7
Reproducibility
Lane 1: Molecular
Weight Markers
Lanes 2–11: Replicates
Figure 2. Ten replicates of a human plasma sample (75 µL) were diluted with 125 µL
of
30 35 40 45 50 55 65 70 75 80 85 90 95
T
em
p
er
at
u
re
(
ºC
)
Time (elapsed, 5-minute intervals)
TC 01 prefilter inlet low TC 02 prefilter inlet high TC 04
p. 56.
7. The Merck Index, 12th ed., Entry# 6352.
8. Conn's Biological Stains, 9th ed., Lillie, R. D.,
Williams and Wilkins (Baltimore, MD: 1977), p. 481.
KAA,HLD,RXR,MAM 04/07-1
version: October 2017
Set of 75 short hydrolysis probes for gene expression quantification of multiple organism levels by
real-time PCR
Cat. No. 04 869 877 001 1 set
75 Universal ProbeLibrary probes
Version: April 2021
Deoxyribonuclease I
Enzyme solution with 10x Incubation Buffer.
Cat. No. 04 716 728 001 10,000 U
10 U/μl
Store the product at −15 to −25°C.
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