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The Effective Use of Protein Kinase Inhibitors
96 72 71 79 93 106
56 103 13 5 2 62 39 19 92 88 40 92 98 55 57 93 94 51 74 88 59 14
96 85 102 101 76 50 58 77 87 76
The Effective Use of Protein Kinase Inhibitors
96 72 71 79 93 106
56 103 13 5 2 62 39 19 92 88 40 92 98 55 57 93 94 51 74 88 59 14
96 85 102 101 76 50 58 77 87 76
Data Sheet - P9542
ml Histone H1 substrate, and trace
[32P]-γ-ATP.
Purity: >95% (SDS-PAGE)
Calculated molecular mass: 78.4 kDa
(apparent molecular mass: 77–84 kDa)1
Precautions and Disclaimer
This product is for
Data Sheet - SRP5132
glutathione, 0.1 mM EDTA,
0.25 mM DTT, 0.1 mM PMSF,and 25% glycerol.
Molecular mass: ∼77 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Precautions and Disclaimer
This product is for R&D use only
Spare parts for Mobius®Bioreactors (50 L, 200 L, 1000 L & 2000 L)
86
1
5
76
50
97
96 87
94
95
76
2
84
78
79
51
3
80
82
4
Data Sheet - SRP5189
sequence of a stress-inducible 40-kDa
protein in HeLa cells. Cell Struct. Funct., 17(1), 77-
86 (1992).
2. Kobayashi, Y. et al., Chaperones Hsp70 and Hsp40
suppress aggregate formation and apoptosis
Data Sheet - SRP5206
70–95% (densitometry)
References
1. Engel, T. et al., Elevated p53 and lower MDM2
expression in hippocampus from patients with
intractable temporal lobe epilepsy. Epilepsy Res.,
77(2-3),151-
Data Sheet - G7534
Biochem., 48, 193
(1979).
2. Gregory, H., Nature, 257, 325 (1975).
3. George-Nascimento, C., Biochemistry, 27, 797
(1988).
4. Todaro, G., et al., Proc. Natl. Acad. Sci. USA, 77,
5258 (1980).
5. Blomquist
MOUSE ANTI-INFLUENZA A MONOCLONAL ANTIBODY
No reactivity shown with the following H1N1 strains: A/Taiwan/1/86;
A/Texas/36/91; A/USSR/90/77; All CA strains 1986 to present.
No reactivity shown to Influenza B strains.
IMMUNOGEN: Influenza
Testing Lab & Industrial Flyer
microscopy (Bot., Hist.) 6409-77-4 Analytical
16019 Phenolphthalein puriss., meets analytical specification of Ph Eur., BP,
98-101% (calc. to the dried substance)
77-09-8 Analytical
81460 Ponceau
Data Sheet - SRP5041
70–95% (densitometry)
Figure 2.
Specific Activity of Typical Lot
77–104 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5 mM
glycerol 2-phosphate
LABORATORY NOTEBOOK ISSUANCE PAGE
07
10
2.
91
13
8.
91
17
8.
49
18
0.
95
18
3.
84
18
6.
21
19
0.
23
19
2.
22
(2
27
)
(2
65
)
(2
68
)
(2
71
)
(2
70
Data Sheet - P1782
28498 (1995).
6. Epand, R.M., Anal. Biochem., 218(2), 241-247
(1994).
7. Dekker, L.V., and Parker, P.J., Trends Biochem.
Sci., 19(2), 73-77 (1994).
8. Nishizuka, Y., Science, 258(5082), 607
LABORATORY NOTEBOOK ISSUANCE PAGE
07
10
2.
91
13
8.
91
17
8.
49
18
0.
95
18
3.
84
18
6.
21
19
0.
23
19
2.
22
(2
27
)
(2
65
)
(2
68
)
(2
71
)
(2
70
LABORATORY NOTEBOOK ISSUANCE PAGE
07
10
2.
91
13
8.
91
17
8.
49
18
0.
95
18
3.
84
18
6.
21
19
0.
23
19
2.
22
(2
27
)
(2
65
)
(2
68
)
(2
71
)
(2
70
Product Information Sheet - N8002
ready to coverslip. If using a permanent
mounting medium, dehydrate in 2 changes of 95%
alcohol, 2 changes of absolute ethanol, and 2
changes of xylene. Slides can now be mounted
using a synthetic, permanent
Product Information Sheet-11888412001
according to the
recommendations of the manufacturer.
2
3
4
5
1
2
3
https://www.sigmaaldrich.com
11
21
.1
19
77
43
Data Sheet - P8538
Conventional Yes Yes 76.9 80 alpha pseudosubstrate peptide, Histone
gamma Conventional Yes Yes 78.4 77–84 alpha pseudosubstrate peptide, Histone
delta Novel No Yes 77.5 74–79 alpha and epsilon pseudosubstrate
Product Information Sheet-dutp-ro
inactivation/cleavage
at abasic sites
95 2 min 1
UNG denaturation 95 2 min 1
Denaturation
Annealing(1)
Elongation(2)
95
60
72
45 sec
60 sec
2 min
25 – 35
MOUSE ANTI-INFLUENZA A
Influenza A H1 antigen.
• Reacts strongly with the following H1N1 strains:
A/Taiwan/1/86, A/USSR/90/77, and all CA strains 1986 to present.
• Does not react well with A/Texas/36/91; or the New Caledonia
HIV - 1 GAG ANTIGEN
DESCRIPTION: HIV-1 gag antigen (recombinant) (a.a. 77-436) of the HIV-1 gag region 39kDa. Reacts
strongly with human I HIV positive serum.
PURITY: >95%; Purity of proteins is evaluated by SDS-PAGE by
Data Sheet - H0160
Type I expressed in and
isolated from Escherichia coli. It contains 358 amino
acid residues (from 77−436) as well as a
β−galactosidase tag (114 kDa) on the N−terminus.
HIV−1 gag (p24) antigen reacts
FcFusionProteinSeparation
.7
86
14
.9
43
15
.2
46
16
.1
14
16
.2
95
16
.4
77
17
.3
66
17
.9
18
Time
(min)
B%
0 0
15 30
55 50
Salt Gradient
Data Sheet - N7529
sequence used for immunization is
100%, 83% and 77% identical in mouse, rabbit and
bovine, respectively.
Reagents
Anti-Cyclic Nucleotide-Gated Cation Channel 2 is
supplied lyophilized at 0.3 mg/ml from
Data Sheet - A0972
solution in 10 mM
NH4HCO3, pH 7.4.
Purity: Minimum 95% (SDS-PAGE).
References
1. Scanu, A.M., et al., FEBS Lett., 18, 305-307 (1971).
2. Scanu, A.M., et al., J. Biol. Chem., 247, 5850-5855
Data Sheet - A5156
water at 20 mg/ml.
References
1. Merck Index, 12th ed., No. 2867 (1996).
2. Meienhofer, J., J. Am. Chem. Soc., 92, 3771-77
(1970).
3. Kleeff, J., et al.; Actinomycin D induces apoptosis
and
Data Sheet - A1410
water at 20 mg/ml.
References
1. Merck Index, 12th ed., No. 2867 (1996).
2. Meienhofer, J., J. Am. Chem. Soc., 92, 3771-77
(1970).
3. Kleeff, J., et al.; Actinomycin D induces apoptosis
and
Data Sheet - A4262
water at 20 mg/ml.
References
1. Merck Index, 12th ed., No. 2867 (1996).
2. Meienhofer, J., J. Am. Chem. Soc., 92, 3771-77
(1970).
3. Kleeff, J., et al.; Actinomycin D induces apoptosis
and
Actinomycin D (A9415)
water at 20 mg/ml.
References
1. Merck Index, 12th ed., No. 2867 (1996).
2. Meienhofer, J., J. Am. Chem. Soc., 92, 3771-77
(1970).
3. Kleeff, J., et al.; Actinomycin D induces apoptosis
and
Specification Sheet
Withstand 0.50 bar (7.25 psi) at 25°C
(77°F)
Operating
Temperature
Tube transfer system: Polyethylene: -20
to 50°C (-4 to 122°F); Silicone: -50 to
95°C (-58 to 203°F), C-Flex®: -73 to
132°C
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