Showing 1-30 of 38 results for "799394"
Daniela Vullo et al.
Bioorganic & medicinal chemistry letters, 21(12), 3591-3595 (2011-05-17)
Two new β-carbonic anhydrases (CAs, EC 4.2.1.1) from the bacterial pathogen Salmonella enterica serovar Typhimurium, stCA 1 and stCA 2, were characterized kinetically. The two enzymes possess appreciable activity as catalysts for the hydration of CO(2) to bicarbonate, with k(cat)...
Katja Biewusch et al.
Methods in molecular biology (Clifton, N.J.), 863, 273-280 (2012-02-24)
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive tumor and still remains a challenge for its lack of effective therapeutic strategies, which is due to the late diagnosis of this disease. Methylation markers might improve early detection and surveillance of PDAC....
Wei Zhou et al.
Journal of Korean medical science, 24(1), 97-103 (2009-03-10)
Epithelial membrane protein 3 (EMP3) is a trans-membrane signaling molecule with important roles in the regulation of apoptosis, differentiation and invasion of cancer cells, but the detailed is largely still unknown. We analyzed the mRNA levels and methylation statuses of...
Miyako Yoshida et al.
Chemical & pharmaceutical bulletin, 61(1), 1-7 (2012-11-06)
The purpose of this study was to predict the stability of imipenem in a mixed infusion. The hydrolysis of imipenem in aqueous solution was found to be accelerated by pH, and by increasing concentrations of sodium bisulfite (SBS) and L-cysteine....
Anna Ohradanova et al.
Bioorganic & medicinal chemistry letters, 22(3), 1314-1316 (2012-01-10)
An α-carbonic anhydrase (CA, EC 4.2.1.1) isolated from the living fossil sponge Astrosclera willeyana, Astrosclerin, was investigated for its inhibition profile with simple inorganic anions, complex anions and other small molecules known to interact with these zinc enzymes. Astrosclerin is...
T Q Lan et al.
Bioresource technology, 127, 291-297 (2012-11-10)
Lodgepole wood chips were pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) at 25% solids loading and 180 °C for 20 min with sulfuric acid and sodium bisulfite charges of 2.2 and 8 wt/wt% on an oven-dry wood...
Ziqiang Meng et al.
Chemosphere, 89(5), 579-584 (2012-07-06)
To study the biological role of bisulfite on vascular contractility and its underlying cellular and molecular mechanisms, to explore whether bisulfite can be used as a sulfur dioxide (SO(2)) donor in the biological experiments, the vasorelaxant effects of sodium bisulfite...
J Krishna Leela et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(1), 258-263 (2012-12-20)
Two pathways of transcription termination, factor-independent and -dependent, exist in bacteria. The latter pathway operates on nascent transcripts that are not simultaneously translated and requires factors Rho, NusG, and NusA, each of which is essential for viability of WT Escherichia...
Shanchun Guo et al.
PloS one, 7(8), e43610-e43610 (2012-09-07)
TREX (transcription/export) is a multiprotein complex that plays a key role in the transcriptional elongation and transport of mRNA from the nucleus to the cytoplasm. We previously reported the purification of the human TREX protein and found that expression of...
Ja-Lok Ku et al.
Methods in molecular biology (Clifton, N.J.), 791, 23-32 (2011-09-14)
DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant...
Shiro Iwagami et al.
Annals of surgical oncology, 19(8), 2726-2732 (2011-12-22)
Genome-wide DNA hypomethylation plays a role in genomic instability and carcinogenesis. DNA methylation in the long interspersed nucleotide element 1 L1 (LINE-1) repetitive element is a good indicator of global DNA methylation level. LINE-1 methylation is a useful marker for...
Synthesis of two new arsenolipids and their identification in fish.
Arroyo-Abad U, et al.
European Journal of Lipid Science and Technology, 118(3), 445-452 (2015)
Alexander Unterberger et al.
Methods in molecular biology (Clifton, N.J.), 863, 303-317 (2012-02-24)
The disruption and alteration of genomic methylation patterns is a hallmark of cancer and other disease states. Understanding and characterizing genome-wide methylation will have a profound effect on our understanding of tumorigenesis and provide novel avenues for therapy. This chapter...
Priyanka Sharma et al.
Gene, 541(1), 31-40 (2014-03-04)
The alteration in the epigenome forms an interface between the genotype and the environment. Epigenetic alteration is expected to make a significant contribution to the development of cardiovascular disease where environmental interactions play a key role in disease progression. We...
Alessandra Cincinelli et al.
Bioorganic & medicinal chemistry, 19(6), 1847-1851 (2011-03-08)
A high activity α-carbonic anhydrase (CA, EC 4.2.1.1) has been purified from various tissues of the Antarctic seal Leptonychotes weddellii. The new enzyme, denominated lwCA, has a catalytic activity for the physiologic CO(2) hydration to bicarbonate reaction, similar to that...
Songjun Guo et al.
Isotopes in environmental and health studies, 48(4), 473-482 (2012-07-20)
δ(13)C values of gaseous acetaldehyde were measured by gas chromatograph-combustion-isotope ratio mass spectrometer (GC-C-IRMS) via sodium bisulfite (NaHSO(3)) adsorption and cysteamine derivatisation. Gaseous acetaldehyde was collected via NaHSO(3)-coated Sep-Pak(®) silica gel cartridge, then derivatised with cysteamine, and then the δ(13)C...
Hamid Mostafavi Abdolmaleky et al.
Methods in molecular biology (Clifton, N.J.), 448, 187-212 (2008-03-29)
Although there is evidence to link schizophrenia (SCZ) and bipolar disorder (BD) to genetic and environmental factors, specific individual or groups of genes/factors causative of the disease have been elusive to the research community. An understanding of the molecular aberrations...
Shuang-Qing Zhang et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 880(1), 163-167 (2011-12-02)
A liquid chromatography-tandem mass spectrometry method for the determination of andrographolide sodium bisulphite (ASB) in rat urine was established and validated. To our knowledge, the analytical method is the first developed assay for the determination of ASB in urine samples....
Sarah H Taylor et al.
BioTechniques, 52(4), 254-262 (2012-04-10)
RNA silencing has been exploited to produce transgenic plants with resistance to viral pathogens via posttranscriptional gene silencing (PTGS). In some cases, this technology is difficult to apply due to the instability of inverted repeat (IR) constructs during cloning and...
Kequan Deng et al.
Molecules (Basel, Switzerland), 17(3), 2968-2979 (2012-03-13)
Defatted soybean flour was treated with hexane and ethanol to reduce lipid content and heated to inactivate lipoxygenase (LOX, linoleate:oxygen reductase; EC 1.13.11.12) to obtain lipid-reduced soybean flour (LRSF). The effects of processing conditions such as pH, reducing agent and...
Yun Huang et al.
Nature protocols, 7(10), 1897-1908 (2012-09-29)
5-Hydroxymethylcytosine (5hmC) is a recently discovered base in the mammalian genome, produced upon oxidation of 5-methylcytosine (5mC) in a process catalyzed by TET proteins. The biological functions of 5hmC and further oxidation products of 5mC are under intense investigation, as...
Paulina Prorok et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), E3695-E3703 (2013-09-12)
Spontaneous hydrolytic deamination of cytosine to uracil (U) in DNA is a constant source of genome instability in cells. This mutagenic process is greatly enhanced at high temperatures and in single-stranded DNA. If not repaired, these uracil residues give rise...
Li-Hui Wang et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 19(5), 675-683 (2006-03-11)
The hedgehog (Hh) signaling pathway plays an important role in foregut development, and its activity is increased in various tumors, including those of the digestive tract. Our objective in the present study was to determine the pattern and extent of...
Eun-Joon Lee et al.
Nucleic acids research, 39(19), e127-e127 (2011-07-26)
We applied a solution hybrid selection approach to the enrichment of CpG islands (CGIs) and promoter sequences from the human genome for targeted high-throughput bisulfite sequencing. A single lane of Illumina sequences allowed accurate and quantitative analysis of ~1 million...
Noriyuki Omura et al.
Cancer biology & therapy, 7(7), 1146-1156 (2008-06-07)
Many genes undergo aberrant methylation in human cancers, and microarray platforms enable more comprehensive profiling of aberrant DNA methylation patterns. 1,010 of 87,922 probes on the 88 K promoter array (606 genes) had a higher signal (log(2) > 2) in...
Katharina Domschke et al.
The international journal of neuropsychopharmacology, 15(9), 1217-1228 (2012-03-23)
The monoamine oxidase A (MAOA) gene has been suggested as a prime candidate in the pathogenesis of panic disorder. In the present study, DNA methylation patterns in the MAOA regulatory and exon 1/intron 1 region were investigated for association with...
Hyung Soo Park et al.
Applied and environmental microbiology, 77(19), 6908-6917 (2011-08-23)
Pipelines transporting brackish subsurface water, used in the production of bitumen by steam-assisted gravity drainage, are subject to frequent corrosion failures despite the addition of the oxygen scavenger sodium bisulfite (SBS). Pyrosequencing of 16S rRNA genes was used to determine...
Christian Otto et al.
Bioinformatics (Oxford, England), 28(13), 1698-1704 (2012-05-15)
Cytosine DNA methylation is one of the major epigenetic modifications and influences gene expression, developmental processes, X-chromosome inactivation, and genomic imprinting. Aberrant methylation is furthermore known to be associated with several diseases including cancer. The gold standard to determine DNA...
Lihong Fan et al.
International journal of biological macromolecules, 50(1), 31-37 (2011-10-15)
Quaternary ammonium chitosan sulfates with diverse degrees of substitution (DS) ascribed to sulfate groups between 0.52 and 1.55 were synthesized by reacting quaternary ammonium chitosan with an uncommon sulfating agent (N(SO(3)Na)(3)) that was prepared from sodium bisulfite (NaHSO(3)) through reaction...
Reaction of sodium bisulfite with uracil, cytosine, and their derivatives.
H Hayatsu et al.
Biochemistry, 9(14), 2858-2865 (1970-07-07)

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