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Analysis of Pesticide Residues in Pistachios Using QuEChERS Extraction and Cleanup with Supel™ QuE Z-Sep+
Pistachios contain approximately 45% fat, which can result in a significant amount of coextracted matrix in the acetonitrile extract generated using the QuEChERS procedure. A zirconia based adsorbent significantly reduces coextracted matrix prior to LC-MS or GC-MS.
Improved Recoveries and Lower Background for GC/MS/MS and LC/MS/MS Analysis of Pesticides in Green Tea
A procedure for cleanup of green tea extracts provides low background and allowed for the analysis of more pesticides at lower levels than QuEChERS. Uses less solvents and no toluene.
Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in Oat-Based Cereals
A simplified LC-MS/MS method to determine glyphosate and related compounds using a Supel™ Carbon LC U/HPLC column for stability under higher pH conditions and sufficient retention for the polar analytes in the presence of methanol as an extraction solvent.
Mesoporous Silica and their Applications
Mesoporous Silica and their Applications
Solvent Systems for Thin-layer Chromatography of Novabiochem Products
A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.
Organosilanes for Cross-coupling
Over the past several years, Pd-catalyzed cross-coupling of silicon compounds has rapidly gained acceptance as a suitable alternative to more commonly known methods such as: Stille (Sn), Kumada (Mg), Suzuki (B), and Negishi (Zn) cross-couplings.
Extractables Study with LC-UV/MS
Reliable identification and quantification of unknown extractables of a single-use system (SUS) with a 21 component CRM mix for liquid chromatography (LC-UV/MS) methods using Ascentis® C18 column.
Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production
Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.
Analysis of Pesticide Residues in Ginger Powder Using QuEChERS Extraction and Clean-up with a Novel Dual Layer SPE Cartridge
The odor and pungency of ginger is due to the presence of terpenes, gingerols and shogaols. These compounds contribute to the highly complex matrix of ginger, which subsequently presents a challenge in low level analyses of contaminants. A novel 2-layer
ALiCE®Cell-Free Protein Synthesis System Protocol
Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
Recent Developments on Hydrogen Release from Ammonia Borane
Record crude oil prices combined with public interest in energy security have resulted in increased attention to a potential transportation economy based on hydrogen fuel.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Working with BRAND® Liquid Handling Instruments
This page covers all essential information regarding working with liquid handling instruments, including dispensers, burettes, and pipettes.
Microbiology Introduction
Introduction to Microbiology Theory. An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man.
Speed, Simplicity and Accuracy of Protein Binding Determination using BioSPME
Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
Dopamine, Norepinephrine and Ephinephrine Synthesis
Dopamine-β-hydroxylase is located inside amine storage vesicles of norepinephrine neurons. Dopamine is actively transported from the cytoplasm into the vesicles. As the enzyme is a copper containing protein, its activity can be inhibited by copper chelating agents, such as diethyldithiocarbamate
Glycosyltransferases: Tools for Synthesis and Modification of Glycans
The presence of multiple functional groups and stereocenters in complex carbohydrates makes them challenging targets for the organic chemist.
Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with Dual-Layer SPE Cartridge
Pesticide residue testing of turmeric and other (dried) spices is challenging due to their complex compositions and high matrix. An efficient SPE clean-up can be achieved by a new Dual-layer cartridge.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Analysis of Pesticide Residues in Cannabis Using QuEChERS Extraction and Cleanup Followed by GC/MS/MS Analysis
Compared to conventional PSA/C18/GCB cleanup, cannabis extracts cleaned with the Supel QuE Verde mixture showed lower GC/MS background, and better recoveries for many pesticides.
Tutorial in Microelectronics and Nanoelectronics
As the electronic industry continues to move towards smaller and smaller devices, researchers must continually strive for technologies to deposit electronic materials with precision at ever-smaller scales. This trend has lead to a variety of techniques used in the microelectronics
Organosilanols
Over the past several years, the Pd-catalyzed cross-coupling of silicon compounds (Hiyama coupling) has rapidly gained acceptance as a suitable alternative to more commonly used methods such as Stille (Sn), Kumada (Mg), Suzuki (B), and Negishi cross-couplings (Zn).
What is the Extracellular Matrix?
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Sample Preparation for Chromatographic Purification
This page discusses various aspects of sample preparation for chromatographic purification.
Development of a Method for Analysis of Phthalate Esters in Vegetable Oils
Rapid, easy, sensitive method for the anaysis of PAEs, using LLE and direct immersion SPME, demonstrated good accuracy, repeatability, linearity and LOQ.
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
Quantification of Methylglyoxal in Manuka Honey - A Simple HPTLC Based Approach
High-Performance Thin-Layer chromatography (HPTLC) quantification of methylglyoxal (MGO) in complex and matrix rich manuka honey offering quick sample preparation, high-matrix tolerance, and high-throughput.
Functionalized PEI and Its Role in Gene Therapy
Gene therapy has become one of the most discussed techniques in biomedical research in recent years.
Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
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