Chemistry (Weinheim an der Bergstrasse, Germany), 13(25), 7085-7090 (2007-06-20)
A fluorescence-based system to sense oxygen in solution is described. The method exploits the sensitivity of the endogenous fluorescence of type-3 copper proteins towards the presence of oxygen by translating the near-UV emission of the protein to label fluorescence in...
Langmuir : the ACS journal of surfaces and colloids, 27(7), 3815-3821 (2011-03-16)
The structure and function of the influenza A M2 proton channel have been the subject of intensive investigations in recent years because of their critical role in the life cycle of the influenza virus. Using a truncated version of the...
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
This article presents a new, highly sensitive method for the identification of single nucleotide polymorphisms (SNPs) in homogeneous solutions using fluorescently labeled hairpin-structured oligonucleotides (smart probes) and fluorescence single-molecule spectroscopy. While the hairpin probe is closed, fluorescence intensity is quenched...
Chembiochem : a European journal of chemical biology, 13(14), 1990-2001 (2012-09-01)
Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for "on/off" experiments. To use FRET to obtain...
Journal of bioenergetics and biomembranes, 40(4), 245-255 (2008-08-01)
The first low resolution solution structure of the soluble domain of subunit b (b (22-156)) of the Escherichia coli F(1)F(O) ATPsynthase was determined from small-angle X-ray scattering data. The dimeric protein has a boomerang-like shape with a total length of...
Dynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-High Density.
Giannone, G.; et al.
Biophysical Journal, 99(4), 1303-1310 (2010)
Flexible Microdomain Specific Staining of Block Copolymers for 3D Optical Nanoscopy.
Ullal, Chaitanya K.; et al.
Macromolecules, 44(19), 7508-7510 (2011)
Dual color localization microscopy of cellular nanostructures.
The journal of physical chemistry. B, 115(17), 5001-5007 (2011-04-12)
Atto655 has been widely used as an excellent probing dye through photoinduced electron transfer (PET) for biochemical processes in oligonucleotides or polypeptides. However, its photophysical properties in the presence of the quenchers guanosine and tryptophan have not been carefully studied....
The Journal of biological chemistry, 286(43), 37768-37777 (2011-09-03)
Pore-forming toxins have evolved to induce membrane injury by formation of pores in the target cell that alter ion homeostasis and lead to cell death. Many pore-forming toxins use cholesterol, sphingolipids, or other raft components as receptors. However, the role...
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 696-703 (2011-01-29)
Intramolecular dynamics in the denatured state of a protein are of importance for protein folding. Native-like contact formation within the ensemble of denatured conformations of a protein may guide its transformation towards the native conformation. The efficiency of folding is...
Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3250-3255 (2008-02-28)
A generic method is described for the fluorescence "readout" of the activity of single redox enzyme molecules based on Förster resonance energy transfer from a fluorescent label to the enzyme cofactor. The method is applied to the study of copper-containing...
Super-Resolution Fluorescence Microscopy.
Huang, B.; Bates, M.; Zhuang, X.
Annual Review of Biochemistry, 78, 993-1016 (2009)
Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 465-469 (2009-04-02)
We introduce a general approach for multicolor subdiffraction-resolution fluorescence imaging based on photoswitching of standard organic fluorophores. Photoswitching of ordinary fluorophores such as ATTO520, ATTO565, ATTO655, ATTO680, or ATTO700, i.e. the reversible transition from a fluorescent to a nonfluorescent state...
2,2?-Thiodiethanol. A new water soluble mounting medium for high resolution optical microscopy.
Staudt, T.; et al.
Microscopy Research and Technique, 70(1), 1-9 (2007)
Investigating Cellular Structures at the Nanoscale with Organic Fluorophores.
van de Linde, S.; et al.
Chemistry and Biology, 20(1), 8-18 (2013)
Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids.
Sahl, S. J.; et al.
Proceedings of the National Academy of Sciences of the USA, 107(15), 6829-6834 (2010)
Single Molecule Characterization of DNA Binding and Strand Displacement Reactions on Lithographic DNA Origami Microarrays.
Scheible, M. B.; Pardatscher, G.; Kuzyk, A.; Simmel, F. C.
Nano Letters, 14(3), 1627-1633 (2014)
Effects of Tuning Fluorophore Density, Identity, and Spacing on Reconstructed Images in Super-Resolution Imaging of Fluorophore-Labeled Gold Nanorods.
Blythe, Karole L.; Titus, Eric J.; Willets, Katherine A
The Journal of Physical Chemistry, 119(50), 28099?28110-28099?28110 (2015)
Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters.
Yamanaka, M.; et al.
Journal of Biomedical Optics, 18(12), 126002-126002 (2013)
Super-Resolution Imaging of Fluorophore-Labeled DNA Bound to Gold Nanoparticles. A Single-Molecule, Single-Particle Approach.
Blythe, Karole L.; Willets, Katherine A.
The Journal of Physical Chemistry C, 120(2), 803?815-803?815 (2016)
The state of oligomerization of the tumor suppressor p53 is an important factor in its various biological functions. It has a well-defined tetramerization domain, and the protein exists as monomers, dimers and tetramers in equilibrium. The dissociation constants between oligomeric...
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2147-2160 (2020-01-08)
Profilin is a major regulator of actin dynamics in multiple specific processes localized in different cellular compartments. This specificity is not only meditated by its binding to actin but also its interaction with phospholipids such as phosphatidylinositol (4,5)-bisphosphate (PIP2 )...
Journal of the American Chemical Society, 133(39), 15568-15577 (2011-08-31)
Photoinduced charge-transfer fluorescence quenching of a fluorescent dye produces the nonemissive charge-separated state, and subsequent charge recombination makes the reaction reversible. While the information available from the photoinduced charge-transfer process provides the basis for monitoring the microenvironment around the fluorescent...
Langmuir : the ACS journal of surfaces and colloids, 28(37), 13395-13404 (2012-08-16)
Fluorescence correlation spectroscopy (FCS) measurements are widely used for determination of diffusion coefficients of lipids and proteins in biological membranes. In recent years, several variants of FCS have been introduced. However, a comprehensive comparison of these methods on identical systems...
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy.
Vogelsang, J.; et al.
Proceedings of the National Academy of Sciences of the USA, 106(20), 8107-8112 (2009)
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde, S.; et al.
Photochemistry and Photobiology, 10(4), 499-506 (2011)
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