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H Brzeska et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(2), 394-399 (1999-01-20)
Acanthamoeba myosin I heavy chain kinase (MIHCK) phosphorylates the heavy chains of amoeba myosins I, increasing their actin-activated ATPase activities. The activity of MIHCK is increased by binding to acidic phospholipids or membranes and by autophosphorylation at multiple sites. Phosphorylation
M Gangal et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(22), 12394-12399 (1999-10-27)
Although the catalytic (C) subunit of cAMP-dependent protein kinase is N-myristylated, it is a soluble protein, and no physiological role has been identified for its myristyl moiety. To determine whether the interaction of the two regulatory (R) subunit isoforms (R(I)
Michael Lorenz et al.
The Journal of biological chemistry, 290(11), 6962-6974 (2015-01-22)
Herpesviruses assemble capsids in the nucleus and egress by unconventional vesicle-mediated trafficking through the nuclear envelope. Capsids bud at the inner nuclear membrane into the nuclear envelope lumen. The resulting intralumenal vesicles fuse with the outer nuclear membrane, delivering the
S P Soltoff
The Journal of biological chemistry, 276(41), 37986-37992 (2001-08-11)
Protein kinase Cdelta (PKCdelta) is activated by stimuli that increase its tyrosine phosphorylation, including neurotransmitters that initiate fluid secretion in salivary gland (parotid) epithelial cells. Rottlerin, a compound reported to be a PKCdelta-selective inhibitor, rapidly increased the rate of oxygen
John M Harrington et al.
The Journal of biological chemistry, 284(20), 13505-13512 (2009-03-28)
Trypanosome lytic factor (TLF) is a subclass of human high density lipoprotein (HDL) that mediates an innate immune killing of certain mammalian trypanosomes, most notably Trypanosoma brucei brucei, the causative agent of a wasting disease in cattle. Mechanistically, killing is
Ksenia Rostislavleva et al.
Science (New York, N.Y.), 350(6257), aac7365-aac7365 (2015-10-10)
Phosphatidylinositol 3-kinase Vps34 complexes regulate intracellular membrane trafficking in endocytic sorting, cytokinesis, and autophagy. We present the 4.4 angstrom crystal structure of the 385-kilodalton endosomal complex II (PIK3C3-CII), consisting of Vps34, Vps15 (p150), Vps30/Atg6 (Beclin 1), and Vps38 (UVRAG). The
Gillian L Dornan et al.
Structure (London, England : 1993), 28(2), 145-156 (2019-12-14)
The class I phosphoinositide 3-kinases (PI3Ks) are key signaling enzymes composed of a heterodimer of a p110 catalytic subunit and a p85 regulatory subunit, with PI3K mutations being causative of multiple human diseases including cancer, primary immunodeficiencies, and developmental disorders.
Sensing phosphatidylserine in cellular membranes
Kay JG and Grinstein S
Sensors, 11(2) (2011)
C M Field et al.
Methods in cell biology, 137, 395-435 (2017-01-10)
We report optimized methods for preparing actin-intact Xenopus egg extract. This extract is minimally perturbed, undiluted egg cytoplasm where the cell cycle can be experimentally controlled. It contains abundant organelles and glycogen and supports active metabolism and cytoskeletal dynamics that closely
Johanna M Jansen et al.
PloS one, 12(4), e0174706-e0174706 (2017-04-07)
RAS mutations lead to a constitutively active oncogenic protein that signals through multiple effector pathways. In this chemical biology study, we describe a novel coupled biochemical assay that measures activation of the effector BRAF by prenylated KRASG12V in a lipid-dependent
Carolina Varela Chavez et al.
Toxins, 8(4), 90-90 (2016-03-31)
Clostridium sordellii lethal toxin (TcsL) is a powerful virulence factor responsible for severe toxic shock in man and animals. TcsL belongs to the large clostridial glucosylating toxin (LCGT) family which inactivates small GTPases by glucosylation with uridine-diphosphate (UDP)-glucose as a
Tomoe Tsuboi et al.
Atherosclerosis, 297, 32-39 (2020-02-18)
Nobiletin (NOB), a functional ingredient found in citrus peel, is said to act against diabetes, obesity, and atherosclerosis. It has been reported to activate AMPK pathway, as well as increase SREBP1c, PPARα and PPARγ expression. However, no molecular mechanism has
Annemieke de Jong et al.
Nature immunology, 15(2), 177-185 (2013-12-24)
T cells autoreactive to the antigen-presenting molecule CD1a are common in human blood and skin, but the search for natural autoantigens has been confounded by background T cell responses to CD1 proteins and self lipids. After capturing CD1a-lipid complexes, we
Jennica Träger et al.
Cells, 9(3) (2020-02-29)
Myelin basic protein (MBP) is located in the insulating covers of nerve cells in the brain and spinal cord. By interacting with lipid membranes, it is responsible for compaction of the myelin sheath in the central nervous system, which is
A E Mc Kiernan et al.
Biophysical journal, 73(4), 1987-1998 (1997-10-23)
It has been hypothesized that nonspecific reversible binding of cytoskeletal proteins to lipids in cells may guide their binding to integral membrane anchor proteins. In a model system, we measured desorption rates k(off) (off-rates) of the erythrocyte cytoskeletal proteins spectrin
Jung Ho Lee et al.
Biochemistry, 55(35), 4949-4959 (2016-07-28)
The interaction between α-synuclein (αS) protein and lipid membranes is key to its role in synaptic vesicle homeostasis and plays a role in initiating fibril formation, which is implicated in Parkinson's disease. The natural state of αS inside the cell
Maryam T Hussain et al.
International journal of pharmaceutics, 573, 118722-118722 (2019-11-11)
Technology such as the use of microfluidics to generate liposomes has been well researched, yet the stabilisation of liposomal formulations is a major challenge to their greater implementation. To the best of our knowledge, this is the first study investigating
Sascha Martens et al.
Science (New York, N.Y.), 316(5828), 1205-1208 (2007-05-05)
Synaptic vesicles loaded with neurotransmitters are exocytosed in a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-dependent manner after presynaptic depolarization induces calcium ion (Ca2+) influx. The Ca2+ sensor required for fast fusion is synaptotagmin-1. The activation energy of bilayer-bilayer fusion
Joury S van 't Klooster et al.
eLife, 9 (2020-04-18)
Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the
The effects of phosphatidylserine on endocrine response to moderate intensity exercise
Starks MA, et al.
Journal of the International Society of Sports Nutrition, 5(1), 11-11 (2008)
Arupratan Das et al.
Nature cell biology, 14(3), 304-310 (2012-02-22)
Lipid asymmetry at the plasma membrane is essential for such processes as cell polarity, cytokinesis and phagocytosis. Here we find that a lipid flippase complex, composed of Lem3, Dnf1 or Dnf2, has a role in the dynamic recycling of the
Katharina Widder et al.
Langmuir : the ACS journal of surfaces and colloids, 34(21), 6095-6108 (2018-05-04)
Interaction of myelin basic protein (MBP) and the cytoplasmic leaflets of the oligodendrocyte membrane is essential for the formation and compaction of the myelin sheath of the central nervous system and is altered aberrantly and implicated in the pathogenesis of
Nathan R Deleault et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8546-8551 (2012-05-16)
Infectious prions containing the pathogenic conformer of the mammalian prion protein (PrP(Sc)) can be produced de novo from a mixture of the normal conformer (PrP(C)) with RNA and lipid molecules. Recent reconstitution studies indicate that nucleic acids are not required
Takaharu Ichimura et al.
The Journal of clinical investigation, 118(5), 1657-1668 (2008-04-17)
Following injury, the clearance of apoptotic and necrotic cells is necessary for mitigation and resolution of inflammation and tissue repair. In addition to macrophages, which are traditionally assigned to this task, neighboring epithelial cells in the affected tissue are postulated
Secil Koseoglu et al.
Analytical chemistry, 87(1), 413-421 (2014-12-03)
The cellular phospholipid membrane plays an important role in cell function and cell-cell communication, but its biocomplexity and dynamic nature presents a challenge for examining cellular uptake of phospholipids and the resultant effects on cell function. Platelets, small anuclear circulating
Małgorzata A Przeradzka et al.
Journal of thrombosis and haemostasis : JTH, 18(2), 364-372 (2019-11-02)
The identity of the amino acid regions of factor VIII (FVIII) that contribute to factor IXa (FIXa) and von Willebrand factor (VWF) binding has not been fully resolved. Previously, we observed that replacing the FVIII C1 domain for the one
Giuseppe Paglia et al.
Nature protocols, 12(4), 797-813 (2017-03-17)
Metabolomics and lipidomics aim to profile the wide range of metabolites and lipids that are present in biological samples. Recently, ion mobility spectrometry (IMS) has been used to support metabolomics and lipidomics applications to facilitate the separation and the identification
Dieter R Klopfenstein et al.
Cell, 109(3), 347-358 (2002-05-23)
Unc104 (KIF1A) kinesin transports membrane vesicles along microtubules in lower and higher eukaryotes. Using an in vitro motility assay, we show that Unc104 uses a lipid binding pleckstrin homology (PH) domain to dock onto membrane cargo. Through its PH domain
Ricarda A Busse et al.
Autophagy, 9(5), 770-777 (2013-03-01)
We characterized phosphoinositide binding of the S. cerevisiae PROPPIN Hsv2 qualitatively with density flotation assays and quantitatively through isothermal titration calorimetry (ITC) measurements using liposomes. We discuss the design of these experiments and show with liposome flotation assays that Hsv2
Soyoun Kim et al.
PloS one, 10(3), e0121171-e0121171 (2015-03-25)
A number of peptide-based indicators have been identified and reported as potential apoptosis probes, offering great promise for early assessment of therapeutic efficacy in several types of cancer. Direct comparison of the newly developed probes with previously used ones would
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