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G G Chang et al.
Archives of biochemistry and biophysics, 296(2), 468-473 (1992-08-01)
The kinetic mechanism of the cytosolic NADP(+)-dependent malic enzyme from cultured human breast cancer cell line was studied by steady-state kinetics. In the direction of oxidative decarboxylation, the initial-velocity and product-inhibition studies indicate that the enzyme reaction follows a sequential...
Wanderson Sirley Reis Teixeira et al.
Talanta, 178, 1040-1045 (2017-11-16)
Methods for determination of glycerol and its electrooxidation products (neutral diols and carboxylates) by capillary electrophoresis (CE) with dual capacitively coupled contactless conductivity detectors (C
A dynamic kinetic asymmetric transformation in the alpha-hydroxylation of racemic malonates and its application to biologically active molecules.
Dhande Sudhakar Reddy et al.
Angewandte Chemie (International ed. in English), 48(4), 803-806 (2008-12-23)
Formation of 2-keto-D-gluconic acid, 5-keto-D-gluconic acid, and tartronic acid by Acetobacter species.
D KULKA et al.
Nature, 167(4257), 905-906 (1951-06-02)
Z Yang et al.
Nature structural biology, 7(3), 251-257 (2000-03-04)
Malic enzymes are widely distributed in nature and have many biological functions. The crystal structure of human mitochondrial NAD(P)+-dependent malic enzyme in a quaternary complex with NAD+, Mn++ and oxalate has been determined at 2.2 A resolution. The structures of...
J Thomas et al.
Annales d'urologie, 23(5), 444-452 (1989-01-01)
The authors study the effects of three acids, one with four carbon atoms, dihydroxymaleic acid, the two others with three carbon considered atoms, ketomalonic acid and tartronic acid. A first study considered the effects of these products on experimental lithiasis...
J Müller
Biochimica et biophysica acta, 830(1), 95-100 (1985-07-18)
Hydroxypyrenetrisulfonate binds to pig mitochondrial malate dehydrogenase (L-malate: NAD+ oxidoreductase, EC in the presence and absence of coenzymes with a stoichiometry of one dye molecule/enzyme subunit. Binding is competitive with substrates and known substrate analogs as well as with...
Christopher D Evans et al.
The Journal of chemical physics, 152(13), 134705-134705 (2020-04-10)
The oxidation of glycerol under alkaline conditions in the presence of a heterogeneous catalyst can be tailored to the formation of lactic acid, an important commodity chemical. Despite recent advances in this area, the mechanism for its formation is still...
Luigi Fiume et al.
Pharmacology, 86(3), 157-162 (2010-08-12)
by reducing the number of ATP molecules produced via aerobic glycolysis, the inhibition of lactic dehydrogenase (LDH) should hinder the growth of neoplastic cells without damaging the normal cells which do not rely on this metabolic pathway for their energetic...
G DuVal et al.
Biochemistry, 24(8), 2067-2072 (1985-04-09)
Some kinetic characteristics of immobilized native mitochondrial malate dehydrogenase dimers and immobilized protomers, prepared by direct immobilization under conditions yielding complete dissociation without substantial unfolding, were compared to those of native soluble enzyme. Enzyme was covalently immobilized to derivatized porous...
J K Hiltunen et al.
Biochimica et biophysica acta, 678(1), 115-121 (1981-11-18)
The mechanism of depletion of tricarboxylic acid cycle intermediates by isolated rat heart mitochondria was studied using hydroxymalonate (an inhibitor of malic enzymes) and mercaptopicolinate (an inhibitor of phosphoenolpyruvate carboxykinase) as tools. Hydroxymalonate inhibited the respiration rate of isolated mitochondria...
Tartronic acid promotes de novo lipogenesis and inhibits CPT-1? by upregulating acetyl-CoA and malonyl-CoA.
Shi, et al.
Life Sciences, 258, 118240-118240 (2020)
K F Petersen et al.
Metabolism: clinical and experimental, 44(11), 1380-1383 (1995-11-01)
The relative roles of pyruvate kinase and malic enzyme in substrate cycling between pyruvate and oxaloacetate were examined in perfused livers of 24-hour-fasted normal and triiodothyronine (T3)-treated rats using an inhibitor of malic enzyme (hydroxymalonate). Livers were perfused for 60...
H Al-Mesfer et al.
Biomaterials, 8(5), 353-359 (1987-09-01)
The syntheses of anhydrosulphite and anhydrocarboxylate derivatives of tartronic acid are described. These compounds, more correctly named 5-carboxy-1,3,2-dioxathiolan-4-one-2-oxide and 5-carboxy-1,3-dioxolan-2,4-dione have been shown to undergo polymerization and copolymerization to poly-alpha-esters containing the tartonic acid residue, characterized by the presence of...
R R Russell et al.
The American journal of physiology, 261(6 Pt 2), H1756-H1762 (1991-12-01)
Acetoacetate, when present as the only fuel for respiration in rat hearts, causes an impairment in contractile function that is reversible with the addition of substrates that can contribute to anaplerosis. To determine the importance of pyruvate carboxylation via NADP(+)-dependent...
I Haro et al.
Journal of enzyme inhibition, 3(3), 189-193 (1990-01-01)
Initial rate kinetic studies of lactate dehydrogenase with ketomalonate and NADH as substrates suggest that this enzymatic system is adapted to a rapid equilibrium ordered bi-bi ternary complex mechanism. The application of the reaction product inhibition method reveals the existence...
S H Park et al.
Biochemistry, 23(23), 5446-5453 (1984-11-06)
Measurement of the initial rate of the malic enzyme reaction varying the concentration of NAD at several different fixed levels of Mg2+ (0.25-1.0 mM) and a single malate concentration gave a pattern which intersects to the left of the ordinate....
J McDevitt et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 29(2), 107-113 (1991-02-01)
In order to estimate the influence of gut bacteria on animal metabolism, the excretion of organic acids, as monitored by gas-liquid chromatography, was compared in the urines of conventional and germ-free rats. Rats were maintained on a chemically simplified diet...
G G Chang et al.
The Biochemical journal, 254(1), 123-130 (1988-08-15)
The pH-induced reversible dissociation of pigeon liver malic enzyme (EC was studied by combined use of chemical cross-linking and SDS/polyacrylamide-gel electrophoresis. The tetrameric enzyme showed a pH-dependent dissociation in an acidic environment. At pH values above 8.0 most molecules...
G Gutierrez et al.
Journal of applied physiology (Bethesda, Md. : 1985), 76(6), 2735-2741 (1994-06-01)
Lactate uptake by skeletal muscle occurs under diverse conditions, including hypoxia and electrical stimulation. A possible metabolic fate of lactate in resting muscle is its conversion to pyruvate followed by carboxylation to malate in the cytosolic malic reaction. To test...
A Shah et al.
Bioorganic & medicinal chemistry, 5(2), 323-334 (1997-02-01)
A new, aromatic analogue of the EPSP synthase enzyme reaction intermediate 1 has been identified, which contains a 3-hydroxymalonate moiety in place of the usual 3-phosphate group. This simplified inhibitor was readily prepared in five steps from ethyl 3,4-dihydroxybenzoate. The...
A Cortés et al.
The Biochemical journal, 357(Pt 1), 263-268 (2001-06-21)
The concentration of an inhibitor that decreases the rate of an enzyme-catalysed reaction by 50%, symbolized i(0.5), is often used in pharmacological studies to characterize inhibitors. It can be estimated from the common inhibition plots used in biochemistry by means...
P A Tipton
Biochemistry, 35(9), 3108-3114 (1996-03-05)
The oxidative decarboxylation of D-malate catalyzed by tartrate dehydrogenase has been analyzed by transient-state kinetic methods and kinetic isotope effect measurements. The reaction time courses show a burst of NADH formation prior to the attainment of the steady-state velocity. The...
Yolande A Chan et al.
Biochemistry, 49(17), 3667-3677 (2010-04-01)
Polyketide synthases elongate a polyketide backbone by condensing carboxylic acid precursors that are thioesterified to either coenzyme A or an acyl carrier protein (ACP). Two of the three known ACP-linked extender units, (2S)-aminomalonyl-ACP and (2R)-hydroxymalonyl-ACP, are found in the biosynthesis...
J Klimek et al.
Journal of steroid biochemistry, 26(1), 161-163 (1987-01-01)
It has been shown that the conversion of cholesterol to progesterone by human term placental mitochondria incubated in the presence of malate or fumarate was inhibited by hydroxymalonate--an inhibitor of malic enzyme. No inhibition was observed when mitochondria were incubated...
The effect of tartronic acid on abnormal fat formation from carbohydrate.
Endocrinology, 47(4), 302-304 (1950-10-01)
[Not Available].
W K RIEBEN et al.
Helvetica physiologica et pharmacologica acta, 4(3), 52-52 (1946-01-01)
P P Tung et al.
Biochimica et biophysica acta, 1545(1-2), 132-145 (2001-05-09)
Cytoplasmic malate dehydrogenase (cMDH) is a key enzyme in several metabolic pathways. Though its activity has been examined extensively, there are lingering mechanistic uncertainties involving substrate and cofactor binding. To more completely understand this enzyme's interactions with cofactor and substrate...