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Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

Enzymatic Assay of 5’-Nucleotidase

To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

Order shRNA Online in 5 Easy Steps

5 easy steps to order shRNA gene of your interest.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Puriﬁcation using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Download our Training Program

Our Training Program is availabale in 5 different languages.

Hydroxymethylfurfural Analysis of Beer by HPLC UV

HPLC-UV determination of 5-HMF (5-(hydroxymethyl)furfural) in beer samples using monolithic silica Chromolith® HighResolution RP-18e HPLC column.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

Protector RNase Inhibitor Protocol

Protector RNase Inhibitor stabilizes RNA at -80°C without permeating intact cells, unsuitable for cell pellet addition.

MilliporeSigma's Bioprocessing 4.0 Technologies

Blazar™ platform wins R&D 100 and CPhI Awards for reducing testing times by up to 80 percent.

5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature

Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.

Accelerating Protein Degrader Discovery with IAP

Plate of 80 ligands against E3 ligase IAP designed by ComInnex; allows creation of bifunctional targeted protein degraders or molecular glues.

Dissecting MOCRA Modernization Cosmetic Regulations Act

MoCRA brings the most significant changes to US cosmetic regulations in 80 years, requiring companies to monitor FDA updates and adopt new compliance policies.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Reflectometric Determination of Hydroxymethylfurfural in Honey

Rapid analysis of 5-(Hydroxymethyl)furfural (HMF) in honey using a reflectometer and Reflectoquant® HMF Test.

Size Exclusion Analysis of Tween-Containing IgG Formulations

In this study, we investigated the effect of adding Tween® 20 and Tween 80 on the elution of proprietary mAb 221 under size exclusion chromatography conditions.

Critical Inhibitor Characteristics to Consider

Discover critical characteristics to consider when working with enzyme inhibitors, such as cell permeability, the prozone effect, and Lipinski’s rule of 5.

Review of O-Linked Glycoproteins

O-Linked glycoproteins are usually large proteins with a molecular mass of >200 kDa. Glycosylation generally occurs in high-density clusters and may represent as much as 50-80% of the overall mass.

Biorepository & Storage

Store your precious material in GMP-compliant facilities amongst three continents. A range of materials including virus and cell banks can be stored in ambient, liquid nitrogen or –80 conditions.

Block Copolymers in Nanoscale Patterning

Block copolymers coat surfaces for nanoscale patterns, improving resolution to 5-50nm, enabling diverse applications like printable circuits.

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Integrity Testers

Efficient Filter Integrity Testing: Elevate quality control with Integritest® 5, offering bubble point, diffusion, and hydrophobic testing in-line or offline.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

QuEChERS Method for Pesticide Residue Analysis

QuEChERS method for pesticide residue analysis requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Determination of Water Content in Phenol Using Karl Fischer Titration

Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.

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