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Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Transmittance to Absorbance Table

Transmittance to absorbance table for fast conversion. Use this chart as a handy reference in the lab, classroom or field. The online transmittance chart is available to download and share.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Flavors and Fragrances New Product Offerings

As a leading global supplier of new flavor and fragrance ingredients, we are dedicated to providing you with high-quality aroma chemicals comprising of certified food grade, IFRA, natural, kosher, and halal ingredients.

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Single-Walled Carbon Nanotubes synthesized by the Super-Growth Method: Properties & Applications

Single-Walled Carbon Nanotubes synthesized by the Super-Growth Method & their properties & applications, including dispersing SGCNTs, SGCNT-polymer composites & SGCNT-metal composites are discussed.

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

Corning® Spheroid Microplates

Understand how Corning® Spheroid Microplates can be used for generating and culturing tumor spheroids in a 96-well spheroid microplate format.

Endothelial Cell Tube Formation Angiogenesis Assay

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Column Packing and Preparation for Size Exclusion Chromatography

MISSION® esiRNA Specifications

MISSION® esiRNA provides RNAi researchers with a proven, cost-effective, and simple way to perform RNAi screens

Starbons: Cooking up Nanostructured Mesoporous Materials

Our strategy is to synthesize mesoporous carbonaceous materials (“Starbons”) using mesoporous expanded starch as the precursor without the need for a templating agent.

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

Pressure-Temperature Calculator for Solvents

Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.

Understanding Sweet Taste Perception

The powerful effect of the pleasurable sensation arising from sweet tasting foods has led to consumer demand and large-scale production of the caloric sweetening agents sucrose and fructose.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

Thermoelectric Performance of Perovskite-type Oxide Materials

The prevailing strategies for heat and electric-power production that rely on fossil and fission fuels are having a negative impact on the environment and on our living conditions.

Self-Assembly in Organic Thin Film Transistors for Flexible Electronic Devices

Flexible electronic circuits and displays based on organic active materials are future generations of products that may eventually enter mainstream electronics market.

MISSION™ CRISPRa Synergistic Activation Mediator (SAM)

2D MXenes Ink: Synthesis, Properties, and Their Precursors

Optimizing the synthesis of high-quality 2D MXene flakes for 3D ink printing is essential to such technological developments as printable and flexible electronics.

Absorbance to Transmittance Converter

Convert absorbance to transmittance values and vice versa.

Graphene-Based Composites and their Unique Renewable Energy Applications

Graphene is a unique two-dimensional (2D) structure of monolayer carbon atoms packed into a dense honeycomb crystal that has attracted great interest due to its diverse and fascinating properties.

TRC Library Citations

TRC 1.5 human and mouse shRNA libraries

HPLC Method Transfer Calculator

Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.

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